Nasal polyp inflammation. Effect of topical nasal steroid.

Kanai, Naoki; Denburg, Judah A.; Jordana, Manel; Dolovich, J.

doi:10.1164/ajrccm.150.4.7921442

Cited by 66 publications

(39 citation statements)

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“…The nasal polyp is a paradigm to mimic airway inflammatory diseases, in as much as it consists essentially of an oedematous stroma with numerous inflammatory cells, including neutrophils and most commonly eosinophils, plasma cells, mast cells, lymphocytes, and macrophages (29,30) covered by a respiratory type epithelium with large areas of basal and mucous cell hyperplasia or even squamous cells metaplasia (12). In fact, neither RT/PCR analysis of CFTR mRNA nor immunodetection of CFTR protein allowed us to detect any striking differences in the non-CF and CF nasal polyps to identify the AF 508 homozygosity.…”

Section: Discussionmentioning

confidence: 99%

CFTR and differentiation markers expression in non-CF and delta F 508 homozygous CF nasal epithelium.

Dupuit¹,

Kälin²,

Brézillon³

et al. 1995

J. Clin. Invest.

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Human nasal polyps from non-CF and AF 508 homozygous CF patients were used to compare the expression of CFTR and markers of epithelial differentiation, such as cytokeratins (CK) and desmoplakins (DP), at the transcriptional and translational levels. mRNA expression was assessed by semiquantitative RT/PCR kinetic assays while the expression and distribution of proteins were evaluated by immunofluorescence analysis. In parallel, for each nasal tissue specimen, the importance of surface epithelium remodeling and inflammation was estimated after histological observations. Our results show that the steady-state levels of CFTR, CK13, CK18, CK14, or DP 1 mRNA transcripts in AF 508 CF nasal polyps were not significantly different from those of non-CF tissues. A variability in the CFTR mRNA transcript level and in the pattern of CFTR immunolabeling has been observed between the different tissue samples. However, no relationship was found between the level of CFTR mRNA transcripts and the CFTR protein expression and distribution, either in the non-CF or in the CF group. The histological observations of non-CF and CF nasal polyp tissue indicated that the huge variations in the expression and distribution of the CFTR protein were associated with the variations in the degree of surface epithelium remodeling and inflammation in the lamina propria. A surface epithelium, showing a slight basal cell hyperplasia phenotype associated with diffuse inflammation, was mainly characterized by a CFTR protein distribution at the apex of ciliated cells in both non-CF and CF specimens. In contrast, in a remodeled surface epithelium associated with severe inflammation, CFTR protein presented either a diffuse distribution in the cytoplasm of ciliated cells, or was absent. These results suggest that abnormal expression and distribution of the CFTR protein in CF airways is not only caused by CFTR mutations. Airway surface epithelium remodeling and inflammation could play a critical role in the posttranscriptional and/ or the posttranslational regulation of the CFIR protein expression in non-CF and CF airways. (J. Clin. Invest. 1995Invest. . 95:1601Invest. -1611

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Section: Discussionmentioning

confidence: 99%

CFTR and differentiation markers expression in non-CF and delta F 508 homozygous CF nasal epithelium.

Dupuit¹,

Kälin²,

Brézillon³

et al. 1995

J. Clin. Invest.

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“…These inflammatory changes are similar to those observed in the inflamed bronchial mucosa in asthma [2, 3, 4]and are characteristic of polyp tissue whether or not allergy is present. It has been observed that the density of eosinophils in nasal polyp tissues is much greater than in control inferior turbinate tissues and that three quarters of eosinophils in the tissue are activated, as indicated by immunohistochemistry positivity with the monoclonal antibody EG2 [5]. …”

Section: Introductionmentioning

confidence: 99%

Measurement of Inflammatory Mediators of Mast Cells and Eosinophils in Native Nasal Lavage Fluid in Nasal Polyposis

Lorenzo¹,

Drago²,

Pellitteri³

et al. 2001

Int Arch Allergy Immunol

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Background: Nasal polyposis (NP) often coexists with asthma, rhinitis and sinusitis. Polyp histology typically shows chronic, eosinophilic inflammation. The inflammatory cell infiltrate generally includes eosinophils, lymphocytes, plasma cells and mast cells. Objective: To gain insight into the natural history of NP, we analysed mediator levels and leukocyte values in nasal fluids and eosinophil cationic protein (ECP), total IgE levels and eosinophils in the blood in several groups of both allergic and non-allergic patients with nasal polyps and in patients with allergic rhinitis (AR). Methods: Thirty-two patients with nasal polyps entered the study. As a control group, we studied 55 patients with AR, i.e. 20 patients with seasonal AR to grass pollen, 24 with AR sensitive to Parietaria and 11 with AR sensitive to house dust mite (HDM). Eighteen patients with nasal polyps were also allergic patients (8 were sensitive to Parietaria and 10 were sensitive to HDM), whereas 14 were non-allergic patients. Tryptase and histamine values were assayed in nasal fluids, whereas total IgE was determined in serum. ECP values were assayed both in nasal fluids and serum. Eosinophils were quantified both in the blood and nasal fluids. Results: Tryptase levels were significantly higher in the nasal lavages from patients with NP than in those from patients without NP (4.7 vs. 3.5 U/l, p < 0.001) and correlated with symptom scores (r_s = 0.42, p < 0.0001). The median levels of histamine in nasal fluids from patients with NP were also significantly higher than those observed in patients without NP (50.0 vs. 21.3 ng/ml, p < 0.001), but did not correlate with symptom scores. Finally, the median levels of ECP in nasal fluids from patients with NP were significantly higher than those observed in patients without NP (38.1 vs. 16.1 ng/ml, p < 0.001) and correlated with symptom scores (r_s = 0.38, p < 0.001). Analysis of variance showed that, among the variables studied, the presence of nasal polyps was the factor responsible for the higher levels of tryptase, histamine and ECP in nasal fluids. With regard to leukocyte counts in nasal fluids, no significant differences were observed between rhinitis patients with NP and those without NP. With regard to serum ECP and serum total IgE, no significant differences were detected between the two groups under study. Blood eosinophil levels in patients with NP were significantly higher than those observed in patients without NP (5.8 vs. 5.6, p = 0.002). Analysis of variance showed that both the presence of nasal polyps and the type of sensitisation were important. Considering the total symptom scores, no significant differences were observed between rhinitis patients with NP and those without NP. Conclusions: The present findings are consistent with the view that chronic eosinophil mucosal inflammatory disease in NP involves a self-sustaining mechanism, i.e. local release of inflammatory mediators, independent of allergen stimulation of nasal mucosa. Increased release of inflammat...

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“…CD34 is expressed most strongly on primitive hemopoietic cells, and is progressively lost as cells differentiate (Strauss et al, 1986). Airway inflammation in patients with asthma and nasal polyps is characterized by the increased presence of several cell types, including mast cells, lymphocytes, and, in particular, eosinophils (Kanai et al, 1994). The aim of this study was to determine the immunohistochemical and histopathological changes on the nasal mucosa after propineb application.…”

Section: Introductionmentioning

confidence: 99%

Effects of Polymeric Zinc Propylen-Bis-Dithiocarbamate (Propineb) on Nasal Mucosa in Rats

Samanci¹,

Arslan²,

Samanci

et al. 2016

Int. J. Morphol.

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SAMANCI, B.; ARSLAN, E.; SAMANCI, S. B.; CAYPINAR, B.; DEVECI, E.; SÖKER, S. & SEKER, U.Effects of polymeric zinc propylen-bis-dithiocarbamate (propineb) on nasal mucosa in rats. Int. J. Morphol., 34(1):85-89, 2016. SUMMARY:The objective of this study was to evaluate the histopathologic and immunohistochemical effects of propineb on rat nasal mucosa. Twenty adult Sprague-Dawley rats weighing 180-220 g, were used as experimental animals. The rats were divided into propineb and control groups. The control group received distilled water with spray at the same time period. The experiment was terminated after three weeks. In each case, sections of the nosewere taken. In experimental group, microscopic examination of nasal respiratory mucosa revealed that degenerative changes in epithelium were observed in sections of propineb-treated group. There were also leukocyte infiltration and vascular dilatation detected in the connective tissue.We detected CD34-immunoreactive mononuclear cells and endothel cells in the lamina propria of propineb group. In propineb group compared to the control group, the respiratory epithelium, goblet and basal cell nuclei were stained positive for PCNA. Propineb inhalation may be irritating to the nasal mucosa.

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Nasal polyp inflammation. Effect of topical nasal steroid.

Cited by 66 publications

References 0 publications

CFTR and differentiation markers expression in non-CF and delta F 508 homozygous CF nasal epithelium.

CFTR and differentiation markers expression in non-CF and delta F 508 homozygous CF nasal epithelium.

Measurement of Inflammatory Mediators of Mast Cells and Eosinophils in Native Nasal Lavage Fluid in Nasal Polyposis

Effects of Polymeric Zinc Propylen-Bis-Dithiocarbamate (Propineb) on Nasal Mucosa in Rats

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