Nasal swab is a good alternative sample for detecting SARS-CoV-2 with rapid antigen test: A meta-analysis

Xie, Jia-Wen; Zheng, Ya-Wen; Wang, Mao; Lin, Yong; He, Yun; Lin, Li‐Rong

doi:10.1016/j.tmaid.2023.102548

Cited by 3 publications

(2 citation statements)

References 57 publications

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“…Given its rapid and easy-to-use technique, RAT is applied largely in COVID-19 POCT as well as in the self-testing setting. However, the low sensitivity at disease onset hinders RAT from becoming an ideal disease-control tool 12,13 .…”

Section: Discussionmentioning

confidence: 99%

“…Further, equipment costs for isothermal ampli cation are much lower than that of PCR. Isothermal nucleic acid ampli cation technology provides a rapid and simple experimental approach compared to the rapid antigen test (RAT); however, its sensitivity and speci city are far superior, making it a promising technology to develop low-cost diagnostic tools 12,13 .…”

Section: Introductionmentioning

confidence: 99%

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Rapid and accurate detection of SARS-CoV-2 using the RHAM technology

Xiao¹,

Liu²,

Kang³

et al. 2023

Preprint

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Rapid and sensitive detection of pathogens is of utmost importance in interrupting the transmission chain of infectious diseases. In recent years, this has proven to be vital during the coronavirus disease (COVID-19) global pandemic that put countless lives at risk. Numerous molecular diagnostic methods were used, including RT-PCR, NASBA, E-SDA, E-RCA, LAMP, and RPA. However, these technologies potentially require primer optimization and complex instruments. Here, we propose the RHAM (RNase Hybridization-Assisted amplification) system as a rapid, specific, and sensitive molecular diagnosis platform. Combining the LAMP and RNase HII-mediated fluorescent reporter, the RHAM system can amplify and visualize the target in one isothermal system with high sensitivity (5×102 copies/mL). There was no cross-reactivity with other common respiratory viruses. Analysis of clinical samples revealed the RHAM system to generate positive signals within 15 minutes without false positive or negative results. The present study shows that RHAM is not only an ideal platform for detecting pathogens, such as SARS-CoV-2 but can be potentially applied in POCT settings.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid and accurate detection of SARS-CoV-2 using the RHAM technology

Xiao¹,

Liu²,

Kang³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Use of dried blood spots in the detection of coronavirus disease 2019 (COVID-19): A systematic review

Alquero,

Estanislao,

Hermino

et al. 2024

Indian Journal of Medical Microbiology

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Rapid and accurate detection of SARS-CoV-2 using the RHAM technology

Xiao,

Liu,

Kang

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Rapid and sensitive detection of pathogens is of utmost importance in interrupting the transmission chain of infectious diseases. In recent years, this has proven to be vital during the coronavirus disease (COVID-19) global pandemic that put countless lives at risk. Numerous molecular diagnostic methods were used, including RT-PCR, NASBA, E-SDA, E-RCA, LAMP, and RPA. However, these technologies potentially require primer optimization and complex instruments. Here, we propose the RHAM (RNase Hybridization-Assisted amplification) system as a rapid, specific, and sensitive molecular diagnosis platform. Combining the LAMP and RNase HII-mediated fluorescent reporter, the RHAM system can amplify and visualize the target in one isothermal system with high sensitivity (5 × 102 copies/mL). There was no cross-reactivity with other common respiratory viruses. Analysis of clinical samples revealed the RHAM system to generate positive signals within 15 min without false positive or negative results. The present study shows that RHAM is not only an ideal platform for detecting pathogens, such as SARS-CoV-2 but can be potentially applied in POCT settings.

show abstract

Nasal swab is a good alternative sample for detecting SARS-CoV-2 with rapid antigen test: A meta-analysis

Cited by 3 publications

References 57 publications

Rapid and accurate detection of SARS-CoV-2 using the RHAM technology

Rapid and accurate detection of SARS-CoV-2 using the RHAM technology

Use of dried blood spots in the detection of coronavirus disease 2019 (COVID-19): A systematic review

Rapid and accurate detection of SARS-CoV-2 using the RHAM technology

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