Nasogastric feeding tubes from a neonatal department yield high concentrations of potentially pathogenic bacteria— even 1 d after insertion

Petersen, Sandra Meinich; Greisen, Gorm; Krogfelt, Karen A.

doi:10.1038/pr.2016.86

Cited by 33 publications

(54 citation statements)

References 27 publications

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“…Premature babies will be fed breast milk, or formula through the tube since the normal suckling reaction is not apparent in preterm babies. Previous studies have shown that the tubes quickly become colonised by bacteria and fungi 11 , 12 . Such tubes are often only semi-transparent and thin, such that bacterial biofilm formation within the tube cannot be visually evaluated.…”

Section: Introductionmentioning

confidence: 98%

Rapid in situ imaging and whole genome sequencing of biofilm in neonatal feeding tubes: A clinical proof of concept

Ogrodzki

Cheung

Saad

et al. 2017

Sci Rep

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The bacterial flora of nasogastric feeding tubes and faecal samples were analysed for a low-birth weight (725 g) neonate EGA 25 weeks in intensive care. Samples were collected at age 6 and 8 weeks of life. Optical coherence tomography (OCT) was used to visualise bacterial biofilms inside the nasogastric feeding tubes. The biofilm was heterogeneously distributed along the tube lumen wall, and had a depth of up to 500 µm. The bacterial biofilm and faecal samples included Enterococcus faecalis and Enterobacter hormaechei. Representative strains, recovered from both feeding tubes and faecal samples, were whole genome sequenced using Illumina, Mi-Seq, which revealed indistinguishable strains, each with less than 28 SNP differences, of E. faecalis and E. hormaechei. The E. faecalis strains were from two sequence types (ST191 and ST211) and encoded for a number of traits related to biofilm formation (BopD), adherence (Epb pili), virulence (cps loci, gelatinase, SprE) and antibiotic resistances (IsaA, tetM). The E. hormaechei were all ST106, and encoded for blaACT-15 β–lactamase and fosfomycin resistance (fosA). This proof of concept study demonstrates that bacterial flora within the neonatal feeding tubes may influence the bacterial colonisation of the intestinal tract and can be visualised non-destructively using OCT.

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Section: Introductionmentioning

confidence: 98%

Rapid in situ imaging and whole genome sequencing of biofilm in neonatal feeding tubes: A clinical proof of concept

Ogrodzki

Cheung

Saad

et al. 2017

Sci Rep

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“…Given the variability in causative organisms for neonatal infection 45 , investigations into potential sources of infection should utilise techniques that can accurately portray all microorganisms. Previous studies investigating contamination have relied on limited culture-based analysis 46 . As previously mentioned, culture-based analysis alone can lead to severe underrepresentation of microbial diversity, which in a clinical scenario could lead to inaccurate targeted therapies.…”

Section: Discussionmentioning

confidence: 99%

Uncovering microbial populations in the lumen of neonatal enteral feeding tubes utilising 16s rRNA sequencing

Winnard

Green

Baylay

et al. 2020

Preprint

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“…The difference in the bacteriological profile of this study from the present study can be explained by the fact that the first culture was sent at 7 th day for all cases. 15 Das et al showed that colonization of Gram negative bacilli (GNB) in neonatal gut is driven mainly by external factors as a stay in the NICU (environmental) and prolonged feeding by a tube. Gastric aspirates and stool sample were examined for gut colonization.…”

Section: Discussionmentioning

confidence: 99%

Bacterial contamination of nasogastric feeding tube and development of neonatal sepsis in premature newborns: a prospective observational research at a tertiary care center in Gujarat, India

Thaddanee¹,

Morbiwala²,

Chauhan³

et al. 2018

Int J Contemp Pediatr

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Background: The objective of the present study is to observe the effect of frequency of changing nasogastric feeding tube (NG-FT) on microbial growth in relation to development of neonatal sepsis in premature newborns. The study is prospective observational study in its nature. Neonatal intensive care unit (NICU) of a tertiary care teaching hospital of western Gujarat, India, from December 2016 to November 2017.Methods: Eighty-five preterm newborns admitted to NICU for feeding support were randomly divided into three groups depending on frequency of changing nasogastric feeding tubes (Group I, II and III with NG-FT changed every 12, 24 and 48 hourly respectively). In Groups I, II and III, the first NG-FT cultures were sent at the end of 12, 24, 48 hours of NG-FT insertion respectively. The second and third NG-FT cultures were sent after 7 and 14 days respectively. Microbial growth pattern was observed and correlated with development of necrotizing enterocolitis (NEC), neonatal sepsis and mortality.Results: Microbial growth on first NG-FT culture significantly increased when frequency of changing nasogastric feeding tube was reduced from every 12 to 24 or 48 hours {p = 0.0432 (Group I vs II) and 0.0001 (Group I vs III)}. Microbial growth increased on second (24.1%, 87% and 85% in groups I, II and III respectively) and third NG-FT culture (44% in group I and 100% in groups II and III); this was significantly high in group II and III as compared to group I (p = 0.0001). Common organisms isolated were Klebsiella pneumonia (63.28%), Pseudomonas aeruginosa (32%) and Escherichia coli (21%). No significant difference was noted in incidences of feeding intolerance, necrotizing enterocolitis (NEC), neonatal sepsis and mortality among the three groups.Conclusions: Organism growth in nasogastric feeding tube culture increases significantly when frequency of changing NG-FT is beyond 12 hours. However, there is no increase in episodes of feeding intolerance, necrotizing enterocolitis (NEC), neonatal sepsis and mortality.

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Nasogastric feeding tubes from a neonatal department yield high concentrations of potentially pathogenic bacteria— even 1 d after insertion

Cited by 33 publications

References 27 publications

Rapid in situ imaging and whole genome sequencing of biofilm in neonatal feeding tubes: A clinical proof of concept

Rapid in situ imaging and whole genome sequencing of biofilm in neonatal feeding tubes: A clinical proof of concept

Uncovering microbial populations in the lumen of neonatal enteral feeding tubes utilising 16s rRNA sequencing

Bacterial contamination of nasogastric feeding tube and development of neonatal sepsis in premature newborns: a prospective observational research at a tertiary care center in Gujarat, India

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