Natriuretic peptide system regulation in granulosa cells during follicle deviation and ovulation in cattle

Abstract

Natriuretic peptides (NPs) are known to regulate reproductive events in polyovulatory species, but their function and regulation in monovulatory species remain to be fully characterized. Using a well-established in vivo model, we found that bovine granulosa cells from follicles near the deviation stage express mRNA for the three NP receptors (NPR1, NPR2 and NPR3), but not for NP precursors (NPPA, NPPB and NPPC). The abundance of NPR3 mRNA was higher in dominant compared to subordinate follicles at the expected time of follicular deviation. After deviation, mRNA for all NP receptors was significantly more abundant in the dominant follicle. Intrafollicular inhibition of oestrogen receptors downregulated NPR1 mRNA in dominant follicles. In granulosa cells from preovulatory follicles, NPPC mRNA increased at 3 and 6 h after systemic GnRH treatment, but decreased at 12 and 24 h to similar levels observed in samples collected at 0 h. After GnRH treatment, NPR1 mRNA was upregulated at 24 h, NPR3 mRNA gradually decreased after 3 h, while NPR2 mRNA was not regulated. The mRNA expression of the enzyme FURIN increased at 24 h after GnRH treatment. These findings revealed that the expression of mRNA encoding important components of the NP system is regulated in bovine granulosa cells during follicular deviation and in response to GnRH treatment, which suggests a role of NP system in the modulation of these processes in monovulatory species.

Rumen-Protected Glucose Stimulates the Insulin-Like Growth Factor System and mTOR/AKT Pathway in the Endometrium of Early Postpartum Dairy Cows

“…Primers were chosen based on the results from previously published papers [15,16] and listed in Table 2. RT-PCR was conducted by a LightCycler480 system (Roche, Castle Hill, NSW, Australia) using the TB Green ® Premix EX Taq TM kit (TaKaRa, Dalian, China).…”

“…The efficiency of PCR amplification for each gene was checked with the dilutions of the samples. The GAPDH was used to normalize the relative expression levels of target genes [16]. The 2 −∆∆Ct method was used for data analysis, as previously described [17,18].…”

Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep

“…Cyclic GMP enters oocyte via GJC and regulates levels of cyclic adenosine monophosphate (cAMP) by suppressing the hydrolyzing activity of oocyte-specific phosphodiesterases 3A (PDE3A). Increased cAMP level maintains meiotic arrest of immature oocytes within follicles [14,[21][22][23]. Besides, it has been stated that estradiol can mediate the expression of NPR2 on CCs [22].…”

Interventions in the B‐type natriuretic peptide signalling pathway as a means of controlling chronic itch

“…They also exhibit greatly attenuated responses to a range of pruritic agents (Mishra & Hoon, 2013;Mishra & Hoon, 2015) NprA Kidney, lung, adipose, adrenal, brain, eye, liver, heart, testis, and vascular smooth muscle tissue, thymus (Fernandez-Durango et al, 1995;Goy et al, 2001;Lowe et al, 1989;Nagase, Katafuchi, Hirose, & Fujita, 1997;Ohsaki, Gross, Le, Oie, & Johnson, 1999;Sarzani et al, 1999;Sekiguchi et al, 2001;Vollmar et al, 1997;Wilcox, Augustine, Goeddel, & Lowe, 1991); granulosa cells (De Cesaro et al, 2018); very low expressed in sensory ganglia (Goswami et al, 2014;Marchenkova et al, 2015;Vilotti et al, 2013;Zhang et al, 2010); gonadal adipose (Neinast et al, 2015); skeletal muscle (Coue et al, 2015); lens epithelial cells (Cammarata et al, 2010); NG108-15 (Muller, Hildebrand, Lubberstedt, Kuhn, & Middendorff, 2010); retina (Rollin et al, 2004); proximal tubular cells (Mistry et al, 2000;Noubani, Farookhi, & Gutkowska, 2000); decidua vera, chorion laeve, myometrium, and placenta (Itoh et al, 1994) NprA −/− mice show high blood pressure, salt sensitivity, volume overload, and cardiac hypertrophy, fibrosis, and inflammation. (Kuhn et al, 2002;Lopez et al, 1995;Oliver et al, 1997;Pandey, 2019) NprB Bone, brain, fibroblasts, heart, kidney, liver, lung, uterine, and vascular smooth muscle tissue; eye (Bryan et al, 2006;Chrisman, Schulz, Potter, & Garbers, 1993;Dickey et al, 2007;Fernandez-Durango et al, 1995;…”

“…NprC Endothelial cells (Leitman et al, 1986); granulosa cells (De Cesaro et al, 2018); adrenal, brain, heart, kidney, liver, mesentery, eye, and vascular smooth muscle tissue (Fernandez-Durango et al, 1995;Nagase et al, 1997;Potter et al, 2006;Vollmar et al, 1997;Wilcox et al, 1991); thymus (Vollmar et al, 1995); DRG and TG (Goswami et al, 2014); white adipose tissue (Smith, Fahrenkrug, Jorgensen, Christoffersen, & Goetze, 2015); skeletal muscle (Coue et al, 2015); lens epithelial cells (Cammarata et al, 2010); α cells (Burgess et al, 2009); retina (Rollin et al, 2004); proximal tubular cells (Mistry et al, 2000); neonatal brain and in isolated primary cortical neurons (Ma & Zhang, 2018) NprC −/− mice have a progressively reduced ability to concentrate urine, exhibit mild diuresis, and tend to be blood volume depleted, skeletal deformities associated with a considerable increase in bone turnover (Matsukawa et al, 1999;Pandey, 2019) T A B L E 2 Differential expression of BNP and its receptors in sensory ganglia and spinal cord…”