Natriuretic peptides stimulate oocyte meiotic resumption in bovine

Cesaro, Matheus Pedrotti de; Macedo, Mariana Priotto de; Santos, Joabel Tonellotto dos; Rosa, Paulo Roberto Antunes da; Ludke, Charles A.; Rissi, Vitor Braga; Gasperin, Bernardo Garziera; Gonçalves, Paulo Bayard Dias

doi:10.1016/j.anireprosci.2015.05.012

Cited by 26 publications

(27 citation statements)

References 42 publications

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“…In Icelandic sheep, the gene NCOA1 can alter the expression of multiple key genes PBP, AIB3 , and FGFR2 , which are important for aberrant labyrinth morphogenesis of the placenta and embryonic lethality ( Chen et al, 2010 ; Huang et al, 2011 ). In Finnsheep, the five candidate genes INHBB, NF1, FLT1, PTGS2 , and PLCB3 played important roles in the development of folliculogenesis and LH signaling ( Ding et al, 2006 ; Tal et al, 2014 ; De Cesaro et al, 2015 ; Ben Sassi et al, 2016 ; Cadoret et al, 2017 ). For example, the INHBB gene encodes an inhibitor of apoptosis, which regulates porcine ovarian follicular atresia ( Terenina et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Analyses Highlight the Potential for Different Genetic Mechanisms for Litter Size Among Sheep Breeds

Gao

Xie

et al. 2018

Front. Genet.

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Reproduction is an important trait in sheep breeding as well as in other livestock. However, despite its importance the genetic mechanisms of litter size in domestic sheep (Ovis aries) are still poorly understood. To explore genetic mechanisms underlying the variation in litter size, we conducted multiple independent genome-wide association studies in five sheep breeds of high prolificacy (Wadi, Hu, Icelandic, Finnsheep, and Romanov) and one low prolificacy (Texel) using the Ovine Infinium HD BeadChip, respectively. We identified different sets of candidate genes associated with litter size in different breeds: BMPR1B, FBN1, and MMP2 in Wadi; GRIA2, SMAD1, and CTNNB1 in Hu; NCOA1 in Icelandic; INHBB, NF1, FLT1, PTGS2, and PLCB3 in Finnsheep; ESR2 in Romanov and ESR1, GHR, ETS1, MMP15, FLI1, and SPP1 in Texel. Further annotation of genes and bioinformatics analyses revealed that different biological pathways could be involved in the variation in litter size of females: hormone secretion (FSH and LH) in Wadi and Hu, placenta and embryonic lethality in Icelandic, folliculogenesis and LH signaling in Finnsheep, ovulation and preovulatory follicle maturation in Romanov, and estrogen and follicular growth in Texel. Taken together, our results provide new insights into the genetic mechanisms underlying the prolificacy trait in sheep and other mammals, suggesting targets for selection where the aim is to increase prolificacy in breeding projects.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Analyses Highlight the Potential for Different Genetic Mechanisms for Litter Size Among Sheep Breeds

Gao

Xie

et al. 2018

Front. Genet.

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“…Disassociated cumulus cells were washed with DPBS and collected after centrifugation at 300 × g for 10 min. Denuded oocytes that were not lysed and completely free of cumulus cells were collected (50 oocytes/pool as per [ 32 ]). Cumulus cells and cumulus-free oocytes were lysed as separate pools in 40 µl 0.1 N HCl before being stored at −80°C.…”

Section: Methodsmentioning

confidence: 99%

Heat stress impairs gap junction communication and cumulus function of bovine oocytes

Campen

Abbott

Rispoli

et al. 2018

Journal of Reproduction and Development

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The intimate association of cumulus cells with one another and with the oocyte is important for regulating oocyte meiotic arrest and resumption. The objective of this study was to determine the effects of heat stress on cumulus cell communication and functions that may be related to accelerated oocyte meiosis during early maturation. Bovine cumulus-oocyte complexes underwent in vitro maturation for up to 6 h at thermoneutral control (38.5°C) or elevated (40.0, 41.0 or 42.0°C) temperatures. Gap junction communication between the cumulus cells and the oocyte was assessed using the fluorescent dye calcein after 4 h of in vitro maturation. Dye transfer was reduced in cumulus-oocyte complexes matured at 41.0°C or 42.0°C; transfer at 40.0°C was similar to control (P < 0.0001). Subsequent staining of oocytes with Hoechst revealed that oocytes matured at 41.0 or 42.0°C contained chromatin at more advanced stages of condensation. Maturation of cumulus-oocyte complexes at elevated temperatures reduced levels of active 5’ adenosine monophosphate activated kinase (P = 0.03). Heat stress exposure had no effect on active extracellular-regulated kinase 1/2 in oocytes (P = 0.67), associated cumulus cells (P = 0.60) or intact cumulus-oocyte complexes (P = 0.44). Heat-induced increases in progesterone production by cumulus-oocyte complexes were detected during the first 6 h of maturation (P = 0.001). Heat-induced alterations in gap junction communication and other cumulus-cell functions likely cooperate to accelerate bovine oocyte meiotic progression.

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“…This study revealed that cumulus cells from low AF cows have higher mRNA expression of NPR‐2 and NPR‐3 than cells from high AF cows. The difference in the mRNA expression of these genes could probably interfere in oocyte maturation, given the role of the NPs in the control of this step (De Cesaro et al., ; Santiquet, Papillon‐Dion, Djender, Guillemette, & Richard, ; Zhang, Su, Sugiura, Xia, & Eppig, ). Considering the delay in the premature resumption of meiosis during IVM in cattle (Franciosi et al., ), it is possible that the time of meiosis progression from the germinal vesicle to the metaphase II stage during IVM differs between oocytes of low and high AF cows, as a result of the variation in the time of the oocyte maturation, cited above.…”

Section: Discussionmentioning

confidence: 99%

Molecular characteristics of granulosa and cumulus cells and oocyte competence in Nelore cows with low and high numbers of antral follicles

Rosa

Marinho

Rosa

et al. 2018

Reprod Domestic Animals

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The aims of this study were to investigate whether the number of antral follicles (AF) in the ovaries of Nelore cows is influenced with the developmental competence of oocytes to reach the blastocyst stage and to quantify the mRNA abundance of genes associated with folliculogenesis and oogenesis in granulosa and cumulus cells. A total of 168 cows were distributed into two experimental groups according to the number of AF, low (≤31) and high AF (≥92), which were determined based on the mean number of AF (61.14) ± SD (30.43). Granulosa and cumulus cells were used to assess the mRNA expression of 16 genes. Cumulus cells from cows with low AF had higher mRNA expression of genes involved in meiosis resumption (NPR-2, NPR-3) and cumulus cell expansion (FGF10), as well as a transcription factor involved in the regulation of oocyte maturation and cell proliferation (STAT3). Conversely, granulosa cells from females with high AF had higher expression of PGR and AMHR2a, which are involved in meiosis resumption and cumulus cell expansion. Cumulus-oocyte complexes (COCs) were collected from 356 cows with low and high AF populations to evaluate embryo development. Cleavage and blastocyst rates did not differ between the groups. In conclusion, our findings revealed that genes involved in folliculogenesis and oogenesis are differently expressed in cumulus and granulosa cells of cows having low and high numbers of AF. These molecular differences suggest that the regulation of oocyte maturation, meiotic resumption and cumulus expansion may be influenced by the number of AFs. However, the variations in gene expression were not associated with in vitro oocyte developmental competence to reach the blastocyst stage, which confirms that oocytes from Nelore cows with low and high numbers of AF are similarly able to mature, regulate the fertilization process and support pre-implantation embryo development.

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Natriuretic peptides stimulate oocyte meiotic resumption in bovine

Cited by 26 publications

References 42 publications

Genome-Wide Association Analyses Highlight the Potential for Different Genetic Mechanisms for Litter Size Among Sheep Breeds

Genome-Wide Association Analyses Highlight the Potential for Different Genetic Mechanisms for Litter Size Among Sheep Breeds

Heat stress impairs gap junction communication and cumulus function of bovine oocytes

Molecular characteristics of granulosa and cumulus cells and oocyte competence in Nelore cows with low and high numbers of antral follicles

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