Natural and artificial substrates for retinal pigment epithelial monolayer transplantation

Singh, Sukhjit; Woerly, S.; McLaughlin, Barbara J.

doi:10.1016/s0142-9612(01)00171-5

Cited by 52 publications

(59 citation statements)

References 21 publications

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“…Natural biomaterial scaffolds include: human amniotic membranes [110][111][112][113][114], human lens capsule [115,116], and explants of Bruch's membrane [117,118]. Using these unaltered natural tissue supports could lead to issues with disease transmission from host and would be difficult to conform to FDA guidelines for transplantation production [119].…”

Section: Transplantation Strategiesmentioning

confidence: 99%

“…Parylene is already approved for use in the eye, and it can be micromachined to include ultrathin areas that mimic the permeability of Bruch's membrane. Hydrogels composed of methacrylate and (meth) acrylamide also promote RPE growth [114]. Current research is making progress toward completely xeno-free derivation of RPE from hESC and iPSC, and discovering the optimal synthetic scaffold would allow the entire process from differentiation to transplantation to be xeno-free.…”

Section: Transplantation Strategiesmentioning

confidence: 99%

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Differentiation of Pluripotent Stem Cells into Retinal Pigmented Epithelium

Croze¹,

Clegg²

2014

Developments in Ophthalmology

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Ocular diseases affect millions worldwide and dramatically influence the quality of life. Although much is known about ocular biology and disease pathologies, effective treatments are still lacking. The eye is well suited for application of emerging cell-based therapies. This chapter explores the development of stem cell-based treatments for age-related macular degeneration (AMD), a prevalent ocular disease in the elderly. Retinal pigmented epithelium (RPE), a cell type implicated in AMD, has been derived from both induced pluripotent stem cells and embryonic stem cells (ESC). Rapidly advancing research has generated various methods of RPE differentiation and several transplantation strategies. Clinical trials are already underway using suspensions of ESC-derived RPE and others are soon to follow. This chapter will provide an overview of current derivation and transplantation strategies for stem cell-derived RPE for the treatment of AMD and other related ocular diseases.

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Section: Transplantation Strategiesmentioning

confidence: 99%

Section: Transplantation Strategiesmentioning

confidence: 99%

Differentiation of Pluripotent Stem Cells into Retinal Pigmented Epithelium

Croze¹,

Clegg²

2014

Developments in Ophthalmology

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“…The two biocompatible polymers, PHEMA and PHPMA, are hydrophilic and present hydroxyl and amide groups, giving a unique combination of properties for cell seeding. [2] The attachment and biological accessibility of the sialyl group in the polymer network was investigated with ELISA and transmission electron microscopy after the binding of Wheat Germ Agglutinin coupled to gold nanoparticles. The deswelling kinetics of the hydrogels were studied using thermogravimetry.…”

Section: Communicationmentioning

confidence: 99%

“…Based on preliminary experiments, this ratio of monomers was chosen to achieve a gel that was mechanically robust for repeated handling. [2] A series of glycosylated hydrogels was prepared with different sugar concentrations by adding 70, 140 and 250 mL of a solution of acrylated 3 0 -sialyllactose (1.3 mg in 0.5 mL distilled water) to the polymerization mixture, keeping constant the ratio of ethylene glycol-water. Gels with a higher sugar concentration were prepared by adding 250 mL of distilled water containing 1 mg of acrylated 3 0 -sialyllactose.…”

Section: Preparation Of Hydrogelsmentioning

confidence: 99%

“…[2,3] In order to promote the adhesion, proliferation and differentiation of cells, a critical requirement in the molecular design of biofunctional polymers is the immobilization of biologically active ligands onto the polymer substrate. Thus, cell adhesion peptides [4,5] or carbohydrates [6] have been coupled to hydrogels to create functional binding interfaces with the cells and to study their effect on cell behavior both in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

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Sialic Acid Engineering of Thin Hydrogel Membranes

Woerly¹,

Fort²,

Heyraud³

2007

Macromol. Rapid Commun.

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We report the synthesis of glycosylated hydrogel membranes of poly{[N‐(2‐hydroxypropyl)methacrylamide]‐co‐(2‐hydroxyethyl methacrylate)} with the aim of developing bioactivated polymer substrates for cell culture. 3′‐Sialyllactose, the saccharidic portion of the GM3 ganglioside involved in cell‐cell recognition over a wide range of biological processes, was chemically modified with an acrylate group and incorporated into the growing macromolecular network of hydrogels by free radical crosslinking copolymerization. The incorporation and accessibility of the sialic acid residues at the hydrogel surface was verified by enzyme linked immunosorbent assay using mouse monoclonal anti‐GM3, and by electron microscopy after labeling with lectin‐gold nanoparticles. The water content was further characterized by thermogravimetry.magnified image

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Biodegradable poly(α‐hydroxy ester) blended microspheres as suitable carriers for retinal pigment epithelium cell transplantation

Thomson

Treharne

Backholer

et al. 2010

J Biomedical Materials Res

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Five blends of poly(L-lactic acid) (PLLA) with poly(D,L-lactic-co-glycolic acid) (PLGA) were used to prepare microcarriers by a solvent evaporation technique. Microcarriers were evaluated as suitable scaffolds to facilitate retinal pigment epithelium (RPE) cell transplantation. The blend ratios were 90:10, 75:25, 50:50, 25:75, 10:90 (PLLA:PLGA). Samples of each microcarrier blend were coated with an extracellular matrix protein. Coated and uncoated microcarriers were seeded with a human RPE cell line. As the lactide unit content increased the size of microcarriers generally became larger and the surface more irregular. Cells remained proliferative and retained phenotype as confirmed by immunocytochemistry. Blends rich in PLLA were superior for maintenance of RPE cell viability. This study demonstrates for the first time the feasibility of using microcarriers as a vehicle for retinal cell transplantation for ocular disease.

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Natural and artificial substrates for retinal pigment epithelial monolayer transplantation

Cited by 52 publications

References 21 publications

Differentiation of Pluripotent Stem Cells into Retinal Pigmented Epithelium

Differentiation of Pluripotent Stem Cells into Retinal Pigmented Epithelium

Sialic Acid Engineering of Thin Hydrogel Membranes

Biodegradable poly(α‐hydroxy ester) blended microspheres as suitable carriers for retinal pigment epithelium cell transplantation

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