Natural and Biomimetic Materials for the Detection of Insulin

Schirhagl, Romana; Latif, Usman; Podlipna, Dagmar; Blumenstock, Hans; Dickert, Franz L.

doi:10.1021/ac201687b

Cited by 96 publications

(50 citation statements)

References 28 publications

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“…A successful route to replace the immunoassays is the synthesis of plastic antibodies [23][24][25]. These materials are prepared by growing a solid polymer structure around a target compound, creating sites that are expected to be complementary in size and electrostatic environment to the imprinted molecule.…”

Section: Introductionmentioning

confidence: 99%

Novel biosensing device for point-of-care applications with plastic antibodies grown on Au-screen printed electrodes

Moreira

Dutra

Noronha

et al. 2013

Sensors and Actuators B: Chemical

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A gold screen printed electrode (Au-SPE) was modified by merging Molecular Imprinting and Self-Assembly Monolayer techniques for fast screening cardiac biomarkers in point-of-care (POC). For this purpose, Myoglobin (Myo) was selected as target analyte and its plastic antibody imprinted over a glutaraldehyde (Glu)/cysteamine (Cys) layer on the gold-surface. The imprinting effect was produced by growing a reticulated polymer of acrylamide (AAM) and N,N * -methylenebisacrylamide (NNMBA) around the Myo template, covalently attached to the biosensing surface. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) studies were carried out in all chemical modification steps to confirm the surface changes in the Au-SPE.The analytical features of the resulting biosensor were studied by different electrochemical techniques, including EIS, square wave voltammetry (SWV) and potentiometry. The limits of detection ranged from 0.13 to 8 µg/mL. Only potentiometry assays showed limits of detection including the cut-off Myo levels. Quantitative information was also produced for Myo concentrations ≥0.2 µg/mL. The linear response of the biosensing device showed an anionic slope of ∼70 mV per decade molar concentration up to 0.3 µg/mL. The interference of coexisting species was tested and good selectivity was observed. The biosensor was successfully applied to biological fluids.

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Section: Introductionmentioning

confidence: 99%

Novel biosensing device for point-of-care applications with plastic antibodies grown on Au-screen printed electrodes

Moreira

Dutra

Noronha

et al. 2013

Sensors and Actuators B: Chemical

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“…According to some researchers, the selectivity of synthetic antibodies (MIPs) toward specific target molecules is found to be comparable to natural antibodies [58]. The regeneration of these synthetic antibodies after every sensing cycle is Figure 2.…”

Section: Synthetic Antibodies: Molecularly Imprinted Polymers For Qcmmentioning

confidence: 92%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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“…Molecular imprinting technology creates materials whose binding specificity is similar to that of antibody-antigen interactions [17,18]. These materials offer unique opportunities in terms of reversible inclusion of a bioanalyte [19], mechanical/chemical stability, low price potentially and compatibility with mass manufactures [18,[20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Protein-responsive polymers for point-of-care detection of cardiac biomarker

Moreira

Sharma

Dutra

et al. 2014

Sensors and Actuators B: Chemical

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This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made respon-sive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing prot ein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications.Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the mate-rial to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The d evices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 µg/mL, respectively, with detection limits of 1.5 and 0.8 µg/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivit y and selectivity.

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Natural and Biomimetic Materials for the Detection of Insulin

Cited by 96 publications

References 28 publications

Novel biosensing device for point-of-care applications with plastic antibodies grown on Au-screen printed electrodes

Novel biosensing device for point-of-care applications with plastic antibodies grown on Au-screen printed electrodes

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Protein-responsive polymers for point-of-care detection of cardiac biomarker

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