Novel biosensing device for point-of-care applications with plastic antibodies grown on Au-screen printed electrodes

Moreira, Felismina T.C.; Dutra, Rosa Amália Fireman; Noronha, João Paulo; Fernandes, João; Sales, M. Goreti F.

doi:10.1016/j.snb.2013.03.099

Cited by 33 publications

(21 citation statements)

References 36 publications

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“…The imprinted material was obtained by electropolymerization of a monomer 5 mM solution in PBS on CA15-3/Au-SPE surface [ 40 ] ( Fig 1D ). The polymerization was carried out by CV measurements with potential range between -0.45 and 0.80V, scan rate of 100 mV/s, for 10 cycles.…”

Section: Methodsmentioning

confidence: 99%

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Sensing CA 15-3 in point-of-care by electropolymerizing O-phenylenediamine (oPDA) on Au-screen printed electrodes

et al. 2018

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This work presents an alternative device for cancer screening in liquid biopsies. It combines a biomimetic film (i) with electrochemical detection (ii). The biomimetic film (i) was obtained by electro-polymerizing amine-substituted benzene rings around a CA 15–3 target. This protein target was previously adsorbed on a gold (Au) support and incubated in charged monomers (4-Styrenesulfonate sodium and 3-Hydroxytyraminium chloride). The protein was further eliminated by enzymatic activity, leaving behind vacant sites for subsequent rebinding. Electrochemical detection (ii) was achieved on an Au working electrode, designed on commercial screen-printed electrodes. Raman spectroscopy, atomic force microscopy and ellipsometric readings were used to follow the chemical modification of the Au surface. The ability of the material to rebind CA15-3 was monitored by electrochemical techniques. The device displayed linear responses to CA15-3 ranging from 0.25 to 10.00 U/mL, with detection limits of 0.05 U/mL. Accurate results were obtained by applying the sensor to the analysis of CA15-3 in PBS buffer and in serum samples. This biosensing device displayed successful features for the detection of CA 15–3 and constitutes a promising tool for breast cancer screening procedures in point-of-care applications. Moreover, its scale-up seems feasible as it contains a plastic antibody assembled in situ, in less than 1 minute, and the analysis of serum takes less than 30 minutes.

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Section: Methodsmentioning

confidence: 99%

“…If a charged species is added into the rebinding site (and only at this site), the recognition may be established by format and differentiated electrostatic interactions. This concept, previously tested before and named SPAM [ 40 ], is tested herein by preparing imprinted materials with charged species.…”

Section: Introductionmentioning

confidence: 99%

Sensing CA 15-3 in point-of-care by electropolymerizing O-phenylenediamine (oPDA) on Au-screen printed electrodes

et al. 2018

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“…Compared with CV method, the EIS can exhibit more dynamic information with a linear response to small amplitude disturbance. [ 16 ] The EIS of molecules can be often described by the Randles model, [ 17 ] which contains four elements: the electrolyte solution resistance ( R s ), the charge transfer resistance ( R ct ), the constant phase element (CPE), and the Warburge impedance ( Z w ). [ 18 ] Here, R s and Z w demonstrate integral property of the solution while R ct and CPE are affected by property at interface between the electrode and the electrolyte.…”

Section: Figurementioning

confidence: 99%

A Novel Biomimetic Magnetosensor Based on Magneto‐Optically Involved Conformational Variation of MagR/Cry4 Complex

Xue

Guo

et al. 2020

Adv Elect Materials

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magnetic field. From the discovery of electromagnetic induction to the quantum Hall effect, humans have strived for seeking applicability of novel mechanisms in the magneto-sensors. [2] Currently, the magneto-sensors are generally based on the quantum effects, which relies on a highly sensitive interface of semiconductor materials commonly fabricated by epitaxial growth. [3] With development of flexible electronics and bioelectronics, these semiconductor based technics seem increasingly unsuitable for the biomedical applications.Interestingly, some species possess an amazing capability of magneto-perception such as migratory birds, [4] butterflies, [5] and honeybees. [6] These animals perceive the geomagnetic field relying on special proteins. Cryptochrome (Cry) has been considered for a long time to mediate the magneto-perception via quantum spin dynamics of light-induced radical-pair, which are excited by sunlight and can be modulated by the geomagnetic field. [7][8][9] This principle was employed to sense the magnetic field because the life-time and the spectral characteristic of radical pair in singlet and triplet states can reflect the information about magnetic field. [10] Cry played an important role in the During billions of years of evolution, some species develop fantastic magnetoperception, offering a biomimetic route to develop the next generation of magneto-sensors. Recently, a novel principle was proposed to explain the navigation of pigeons in the presence of geomagnetic fields and sunlight. The key link is thought to lie in the magneto-optically involved conformational variation of magnetoreceptor protein (MagR)/cryptochrome (Cry4) complex. The MagR/Cry4 complex is fabricated and purified in vitro, creating a magnetosensing device by immobilization of this protein on a graphene-modified electrochemical electrode. By using electrochemical impedance spectroscopy, magneto-sensing with a current detectability of 10 mT is realized with MagR/ Cry4 complex. It is proved that this process requires the involvement of both magnetic fields and light, partly confirming in vitro the magneto-perceptive mechanism of MagR/Cry4 complex. It is also shows that this device can be used to reflect the anisotropic responses of nanomaterials to the external magnetic field. It is believed this protein-based magneto-sensing will greatly boost development of bioelectronics and deepen understanding of the phenomena of magneto-perception for organisms.

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“…Until now, the semi-covalent imprinting was not used for protein templates. There are some examples of MIP fabrication where proteins were covalently immobilized on the substrate surface (Moreira et al, 2013a(Moreira et al, , 2013b(Moreira et al, , 2013cTretjakov et al, 2013). Moreover, cytochrome c was labeled with a cleavable functional monomer bearing a disulfide bond and terminal maleimide moiety capable of binding cys-17 located on the cytochrome c surface (Suga et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Selective electrochemical sensing of human serum albumin by semi-covalent molecular imprinting

Cieplak

Szwabińska

Sosnowska

et al. 2015

Biosensors and Bioelectronics

139

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We devised and prepared a conducting molecularly imprinted polymer (MIP) for human serum albumin (HSA) determination using semi-covalent imprinting. The bis(2,2'-bithien-5-yl)methane units constituted the MIP backbone. This MIP was deposited as a thin film on an Au electrode by oxidative potentiodynamic electropolymerization to fabricate an electrochemical chemosensor. The HSA template imprinting, and then its releasing from the MIP was confirmed by the differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), XPS, and PM-IRRAS measurements as well as by AFM imaging. Semi-covalent imprinting provided a very well defined locations of recognition sites in the MIP molecular cavities. These sites populated the imprinted cavities or the MIP surface only. The DPV and EIS response of the MIP film coated electrode to the HSA analyte was linear in the range of 0.8 to 20 and 4 to 80 µg/mL HSA, respectively, with the limit of detection of 16.6 and 800 ng/mL, respectively. The impressively high imprinting factor reached, exceeding 20, strongly confirmed that semi-covalent imprinting resulted in formation of a large number of very well defined molecular cavities with high affinity to the HSA molecules. The MIP selectivity against low-(molecular weight) interferences, common for physiological fluids, such as blood and urea, was very high. There was no response to the presence of these interferences at concentrations encountered in the samples analyzed. Moreover, the chemosensor selectivity to the myoglobin and cytochrome c interferences was excellent while that to lysozyme was slightly lower but still high. The chemosensor was useful for determination of abnormal HSA concentration in a control blood serum.

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Novel biosensing device for point-of-care applications with plastic antibodies grown on Au-screen printed electrodes

Cited by 33 publications

References 36 publications

Sensing CA 15-3 in point-of-care by electropolymerizing O-phenylenediamine (oPDA) on Au-screen printed electrodes

Sensing CA 15-3 in point-of-care by electropolymerizing O-phenylenediamine (oPDA) on Au-screen printed electrodes

A Novel Biomimetic Magnetosensor Based on Magneto‐Optically Involved Conformational Variation of MagR/Cry4 Complex

Selective electrochemical sensing of human serum albumin by semi-covalent molecular imprinting

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