Natural and Experimental Bovine Immunodeficiency Virus Infection in Cattle

“…The cattle industry in Poland is predominantly composed of dairy cattle with a special location of a number of herds in these areas. Although the mechanism of BIV transmission is unclear, the high density and close housing of cattle has been postulated as a means for a higher rate of infection in dairy herds (Snider et al 1997). Since maintenance and breeding methods are Positive 13 3 n=16 27 326 n=353 Negative 6 1 n=7 50 1138 n=1188 Total 19 7 n=23 77 1464 n=1541 different between dairy and beef cattle, a higher BIV seroprevalence in dairy cattle was noted (Amborsky et al 1989, Polack et al 1996.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that BIV infection of cattle produces only moderate pathological effects (Carpenter et al 1992). However, some reports have linked BIV to encephalitis, clinical immunodeficiency, and transient B-cell proliferation (Snider et al 1997, Whetstone et al 1997. Several reports confirmed that in herds where infection with bovine leukemia virus (BLV) was noted, cattle dually infected with BIV and BLV were found (Amborsky et al 1989, StCyr Coats et al 1994, Jacobs et al 1995.…”

Section: Introductionmentioning

confidence: 99%

“…Several reports confirmed that in herds where infection with bovine leukemia virus (BLV) was noted, cattle dually infected with BIV and BLV were found (Amborsky et al 1989, StCyr Coats et al 1994, Jacobs et al 1995. The significance of this coinfection is not clear, and it was suggested that BLV might serve as cofactor for BIV replication (Snider et al 1997). This assumption was additionally supported by the fact that BIV and BLV share common host cell tropisms, specifically for B-lymphocytes and monocytes (Heaton et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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Serological survey for bovine immunodeficiency virus in dairy cattle from Poland

Rola–Łuszczak¹,

Kozaczynska²,

Kuźmak³

2011

Polish Journal of Veterinary Sciences

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A seroprevalence study of bovine immunodeficiency virus (BIV) was undertaken on 1,541 serum samples from Holstein cattle from 23 herds, located in different geographical regions of Poland. The analysis was performed using ELISA, with recombinant Gag protein of BIV as antigen. The average BIV prevalence was 4.9% in individual cattle, while the percentage of herds harboring at least one seropositive animal, was 82.6%. To demonstrate the correlation of BIV and bovine leukemia virus infection, all sera were analysed for BLV antibodies and there was only a slight association between both infections. Overall, these results show that BIV infection is present in dairy cattle in Poland at a prevalence rate found in other European countries.

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“…Bovine immunodeficiency virus (BIV) is a lentivirus that causes lymphadenopathy, lymphocytosis, central nervous system lesions, progressive weakness, and emaciation (20,21,24). Studies have shown that BIV resembles human immunodeficiency virus (HIV) type 1 and other lentiviruses, e.g., equine infectious anemia virus and feline immunodeficiency virus, in its structural, genetic, antigenic, and biological properties (5,6,16).…”

mentioning

confidence: 99%

Cloning of the Bovine Immunodeficiency Virus gag Gene and Development of a Recombinant-Protein-Based Enzyme-Linked Immunosorbent Assay

Zheng¹,

Swanson²,

Liao³

et al. 2000

Clin Diagn Lab Immunol

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An enzyme-linked immunosorbent assay (ELISA) was established for the rapid detection of specific bovine immunodeficiency virus (BIV) antibodies in cattle, using recombinant Gag protein as an antigen. The gag coding region from BIV was cloned into an expression vector, pQE32, which expressed high levels of recombinant protein from Escherichia coli. The ELISA was standardized by a checkerboard titration against known BIV-positive and -negative sera from cattle and a monoclonal antibody to the Gag protein. A total of 139 cattle serum samples, from the diagnostic laboratory at Kansas State University, Manhattan, and from the Dairy Station, Louisiana State University, Baton Rouge, were compared by ELISA and immunoblot assays for the detection of BIV-specific antibodies. Of 26 cattle sera samples which tested positive using the immunoblot assay, 23 were positive by ELISA, thus establishing a strong correlation between the two tests. The sensitivity and specificity of ELISA relative to immunoblotting were 0.88 and 0.93, respectively. ELISA proved to be as specific as immunoblotting but was much less time-consuming and easier to perform.

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Natural and Experimental Bovine Immunodeficiency Virus Infection in Cattle

Cited by 27 publications

References 68 publications

Isolation of an Avian Serovar ofChlamydia Psittacifrom a Case of Bovine Abortion

Isolation of an Avian Serovar ofChlamydia Psittacifrom a Case of Bovine Abortion

Serological survey for bovine immunodeficiency virus in dairy cattle from Poland

Cloning of the Bovine Immunodeficiency Virus gag Gene and Development of a Recombinant-Protein-Based Enzyme-Linked Immunosorbent Assay

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