Natural and Heterologous Production of Bacteriocins

Cintas, Luis M.; Herranz, Carmen; Hernández, Pablo E.

doi:10.1007/978-1-4419-7692-5_8

Cited by 4 publications

(4 citation statements)

References 120 publications

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“…Those published by the end of July 2011 were collected. Some publications refer to documents which seem to make reference to other documents than those published by EFSA (Delorme et al, 2010;Forssten et al, 2011a;b;Izawa et al, 2011;), others refer to initial QPS documents published by EFSA (reports and opinion up to 2007) rather than to the recently updated QPS opinions (Aureli et al, 2011;Ayeni et al, 2011;Cintas et al, 2011;Doyle and Erickson, 2011;Engel et al, 2011;Kneifel, 2011;Mayrhofer et al, 2011;Moslehi-Jenabian et al, 2010;Salazar et al, 2011;Saxelin et al, 2011;Seitter et al, 2011;Talon and Leroy, 2011) while some refer to more recent QPS opinions as of 2008 (Chanos and Williams, 2011;Chenoll et al, 2010;Clementi and Aquilanti, 2011;Cousin et al, 2011;Ferreira et al, 2011;Johnsson Holmsberg, 2011;Klein, 2011a;Meieregger et al, 2011;Ouwehand et al, 2011;Patel et al, 2010;Sundh and Melin, 2011). …”

Section: 3mentioning

confidence: 99%

Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2011 update)

2011

EFSA Journal

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EFSA is requested to assess the safety of a broad range of biological agents (including microorganisms and viruses) in the context of notifications for market authorisation as sources of food and feed additives, enzymes and plant protection products. The qualified presumption of safety (QPS) assessment was developed by EFSA for its own use to provide a generic risk assessment approach applicable across EFSA's scientific Panels, for biological agents notified for intentional use in the whole food chain. The safety of unambiguously defined biological agents at the highest taxonomic unit that is appropriate for the purpose for which an application is intended and the completeness of the body of knowledge are assessed. Identified safety concerns for a taxonomic unit are where sensible reflected as ‘qualifications’ when a recommendation for the QPS list is given. The list of QPS recommended biological agents is reviewed and updated annually. Therefore, the only valid list is the one in the most recent scientific opinion. The 2011 update reviews microorganisms previously assessed including bacteria, yeasts, filamentous fungi and viruses used for plant protection purposes and confirms the previous recommendations. The anamorph yeast form Phaffia rhodozyma of Xanthophyllomyces dendrorhous was included on the QPS list and to the qualification for yeasts ‘absence of resistance to antimycotics used for medical treatment of yeast infections’, the sentence was added that ‘in the case of Saccharomyces cerevisiae this qualification applies for yeast strains able to grow above 37 °C’. The body of knowledge of filamentous fungi and enterococci was updated and their ineligibility for the QPS list confirmed.

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Section: 3mentioning

confidence: 99%

Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2011 update)

2011

EFSA Journal

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“…6 The development of biotechnology makes the production of native or recombinant bacteriocins more efficient by using synthetic genes and a good expression system. 7 Gene synthesis is a method for synthesizing a concerned gene in vitro without an original DNA template. Furthermore, commercial synthesis of the desired gene is considering a more rapid step, it is time saving and is developed to be a cost effective alternate for classic cloning techniques and other molecular biology processes.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Expression of Synthetic Plantaricin F Gene

Madhi¹,

Khudor²,

Othman³

2023

J. Pure Appl. Microbiol.

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Antimicrobial activity of certain species of Lactobacillus bacteria were known to produce a certain type of bacteriocin called Plantaricin (Pln). In this study, the PlnF (plnF) gene (666 bp) encoding plantaricin was synthetically constructed based on a sequence of predefined and valuable amino acid compositions and subcloned into pET-28 (+) expression vector. Thereafter transformation of recombinant vector into BL21 (DE3) competent E. coli was achieved and to verify the result of cloning, the PCR colony technique was conducted. The expression of the inserted gene was analyzed by the SDS-polyacrylamide gel electrophoresis method, which detected a band of approximately 30 KD molecular weight for interested protein as a natural bioactive bacteriocin.

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“…Bacteriocin production is a common trait of LAB, and many bacteriocinogenic strains have been characterized, displaying antimicrobial activity against a wide range of Gram-positive bacteria, and, to a lesser extent, Gram-negative bacteria, including spoilage and foodborne pathogens. Their spectrum of antimicrobial activity has attracted great interest for their potential as food preservatives and as therapeutical agents in both human and veterinary medicine [ 13 ]. In this regard, Lactococcus lactis subsp.…”

Section: Introductionmentioning

confidence: 99%

Draft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm

et al. 2022

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Probiotics are a viable alternative to traditional chemotherapy agents to control infectious diseases in aquaculture. In this regard, Lactococcus lactis subsp. cremoris WA2-67 has previously demonstrated several probiotic features, such as a strong antimicrobial activity against ichthyopathogens, survival in freshwater, resistance to fish bile and low pH, and hydrophobicity. The aim of this manuscript is an in silico analysis of the whole-genome sequence (WGS) of this strain to gain deeper insights into its probiotic properties and their genetic basis. Genomic DNA was purified, and libraries prepared for Illumina sequencing. After trimming and assembly, resulting contigs were subjected to bioinformatic analyses. The draft genome of L. cremoris WA2-67 consists of 30 contigs (2,573,139 bp), and a total number of 2493 coding DNA sequences (CDSs). Via in silico analysis, the bacteriocinogenic genetic clusters encoding the lantibiotic nisin Z (NisZ) and two new bacteriocins were identified, in addition to several probiotic traits, such as the production of vitamins, amino acids, adhesion/aggregation, and stress resistance factors, as well as the absence of transferable antibiotic resistance determinants and genes encoding detrimental enzymatic activities and virulence factors. These results unveil diverse beneficial properties that support the use of L. cremoris WA2-67 as a probiotic for aquaculture.

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Natural and Heterologous Production of Bacteriocins

Cited by 4 publications

References 120 publications

Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2011 update)

Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2011 update)

Cloning and Expression of Synthetic Plantaricin F Gene

Draft Genome Sequence of Lactococcus lactis Subsp. cremoris WA2-67: A Promising Nisin-Producing Probiotic Strain Isolated from the Rearing Environment of a Spanish Rainbow Trout (Oncorhynchus mykiss, Walbaum) Farm

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