Natural antibiotic susceptibility of strains of the Enterobacter cloacae complex

Stock, Ingo; Grüger, Thomas; Wiedemann, B.

doi:10.1016/s0924-8579(01)00463-0

Cited by 46 publications

(25 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A majority of extended-spectrum ␤-lactamase (ESBL)-and carbapenemase-producing isolates of Enterobacteriaceae are susceptible to fosfomycin (42), with susceptibility in 47 to 72% E. cloacae isolates (EUCAST criteria) (43,44). This isolate was resistant to fosfomycin, consistent with the chromosomally encoded glutathione transferase (fosA).…”

mentioning

confidence: 91%

Carbapenem- and Colistin-Resistant Enterobacter cloacae from Delta, Colorado, in 2015

Norgan

Freese

Tuin

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

eResistance to carbapenems in Enterobacteriaceae is a clinical problem of growing significance. Difficulty in treating multidrugresistant Gram-negative organisms with conventional antibiotics has led to a renewed and increasing use of polymyxin compounds, such as colistin. Here, we report the isolation of carbapenem-and colistin-resistant Enterobacter cloacae from a polymicrobial lower extremity wound in an ambulatory patient. Whole-genome sequencing demonstrated the presence of chromosomal bla IMI-1 and bla AmpC , as well as numerous efflux pump genes.

show abstract

mentioning

confidence: 91%

Carbapenem- and Colistin-Resistant Enterobacter cloacae from Delta, Colorado, in 2015

Norgan

Freese

Tuin

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…E. cloacae is a Gram-negative intestinal commensal bacterium that colonizes 40 to 80% of the human population (16) and has previously been identified as an opportunistic nosocomial pathogen (17)(18)(19). Treatment of infection by E. cloacae can be complicated by its natural resistance to many antibiotics and its ability to acquire resistance to others after exposure (20,21).…”

mentioning

confidence: 99%

Colistin Heteroresistance in Enterobacter cloacae Is Associated with Cross-Resistance to the Host Antimicrobial Lysozyme

Napier

Band

Burd

et al. 2014

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Here, we describe the first identification of colistin-heteroresistant Enterobacter cloacae in the United States. Treatment of this isolate with colistin increased the frequency of the resistant subpopulation and induced cross-resistance to the host antimicrobial lysozyme. This is the first description of heteroresistance conferring cross-resistance to a host antimicrobial and suggests that clinical treatment with colistin may inadvertently select for bacteria that are resistant to components of the host innate immune system.

show abstract

“…Different ranges of concentrations of the earlier mentioned antimicrobials have been incorporated in the MIC study after determination of the MIC value of each antimicrobial through several trials of experiment in various batches. Evaluation of a wide range of antibiotics tested against E. cloacae, Enterobacter hormaechei and Enterobacter asburiae strains, have provided a database for their natural susceptibility (Stock et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Most isolates of the E. cloacae complex are susceptible to fluoroquinolones, trimethoprim/sulfamethoxazole, chloramphenicol, aminoglycosides, tetracyclines, piperacillin-tazobactam and carbapenems, while they are intrinsically resistant to ampicillin, amoxicillin, amoxicillinclavulanate, first-generation cephalosporins and cefoxitin owing to the production of constitutive AmpC β-lactamase. In particular, fosfomycin seems to have a different activity against all species, because E. cloacae and E. asburiae are both naturally susceptible and resistant, while E. hormaechei is only naturally sensitive (Stock et al, 2001). …”

Section: Discussionmentioning

confidence: 99%

Study of resistance mechanism in Enterobacter spp. with special reference to antimicrobials ceftazidime, moxifloxacin and nalidixic acid

M¹,

Bagde²

2015

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Microbial resistance to antimicrobials is spreading all over the world making it difficult to treat diseases effectively. This study aims to understand the mechanism of resistance towards the antimicrobials Ceftazidime, Moxifloxacin and Nalidixic acid, by using resistant Enterobacter spp. and sensitive Enterobacter spp. While there was complete inhibition of growth of sensitive Enterobacter spp. at 8 µg/ml Ceftazidime, 0.125 µg/ml of Moxifloxacin and 16 µg/ml of Nalidixic acid, resistant Enterobacter spp. even tolerated 256, 32 and 1536 µg/ml Ceftazidime, Moxifloxacin and Nalidixic acid respectively. Browthdilution method was used to determine the growth of organisms at different concentrations of antibacterial agents. Mechanism of resistance was found to be present in plasmid. Absence of Beta lactamase enzyme seems to be an important finding in this sudy. B. lactamase enzyme was checked with help of the acidometric and idometric method. Plasmid isolation and analysis was done by agarose gel electrophoresis. Successful curing of plasmid was carried out with 10% sodium dodecyl sulfate (SDS). When colonies after SDS treatment were tested, resistant strains were found which were later converted to sensitive ones. In this study, the resistant Enterobacter spp. executed resistance to three different classes of antimicrobials due to the resistance plasmid. The results obtained in this study support most of the previous study findings who contributed in this field. Therefore, it might be useful to recognize the resistance mechanism, and to determine the correct practicing of drug usage.

show abstract

Natural antibiotic susceptibility of strains of the Enterobacter cloacae complex

Cited by 46 publications

References 12 publications

Carbapenem- and Colistin-Resistant Enterobacter cloacae from Delta, Colorado, in 2015

Carbapenem- and Colistin-Resistant Enterobacter cloacae from Delta, Colorado, in 2015

Colistin Heteroresistance in Enterobacter cloacae Is Associated with Cross-Resistance to the Host Antimicrobial Lysozyme

Study of resistance mechanism in Enterobacter spp. with special reference to antimicrobials ceftazidime, moxifloxacin and nalidixic acid

Contact Info

Product

Resources

About