Natural antioxidants in the vitrification solution improve the ovine ovarian tissue preservation

Vitrification of canine ovarian tissue using the Ovarian Tissue Cryosystem (OTC) device

“…Contrarily, a reduction in stromal cell density was also observed in our study. Similar results have been reported after vitrification of sheep (Morais et al, 2019) and caprine (Carvalho et al, 2013) ovarian tissue. This type of parameter has not been reported in canine ovarian tissue after vitrification.…”

“…Thus, we reiterate the previous hypothesis about oxidative stress arising from the toxicity of cryoprotective agents that may have favoured this decrease. Also, it is known that the permeability of cryoprotective agents, such as EG and DMSO, interacts with ovarian cells and can cause excessive cell dehydration and consequently death (Morais et al, 2019). Some protocols use the cryotube throughout the procedure (Ishijima et al, 2006(Ishijima et al, , 2009Hariya & Suzuki, 2016) or just in the storage of the material in LN (Ackermann et al, 2016;Fujihara et al, 2019;Jivago et al, 2018), which represent a variety of protocols.…”

A novel RANKL‐targeted flavonoid glycoside prevents osteoporosis through inhibiting NFATc1 and reactive oxygen species

“…For example, the highly expressed TLR2 and TLR4, as well as the translocation of NF‐κB p65, induced by the oxidized low‐density lipoprotein were suppressed by the addition of Rob to human peripheral blood mononuclear cells 17 . Furthermore, a previous study showed that Rob, which has excellent antioxidative properties, also had protective effects on ovine ovarian tissue in vitrification preservation 19 …”

“…17 Furthermore, a previous study showed that Rob, which has excellent antioxidative properties, also had protective effects on ovine ovarian tissue in vitrification preservation. 19 Given the important anti-inflammatory and antioxidative effects of Rob in different applications, we hypothesized that Rob could prevent RANKL-induced bone loss and osteoclast formation by inhibiting NFATc1 activity, Ca 2+ ossification, ROS, and NF-κB/MAPK signaling. Therefore, we analyzed the effects of Rob on (i) RANKL-induced osteoclast differentiation and the underlying mechanisms in in vitro experiments, and (ii) bone matrix loss in an ovariectomized (OVX) animal model in vivo.…”

Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species

“…Proper addition of antioxidants can reduce oocyte and embryo damage caused by oxidative stress [20,21]. Studies have shown that CA can improve antioxidant activity in cells, inhibiting oxidative stress caused by H2O2 or other toxic substances in vivo to reduce cell damage.…”