In this paper, in order to improve the efficiency of waste heat utilization, based on the movement type and shape of the snake, snake bionic surfaces with different structures were designed, and CuO–H2O nanofluids were used instead of traditional working fluids, and they were coupled together and applied to waste heat utilization equipment. The main work is to experimentally study the effects of nanofluids mass fraction (w = 0.1 wt%, 0.3 wt%, 0.5 wt%), amplitude of bionic channel structure (A = 1 mm, A = 2 mm, A = 3 mm), angular frequency of bionic channel structure (ω = 20 rad/s, ω = 25 rad/s, ω = 30 rad/s), phase shift of bionic channel structure (α = 0°, 90°, 180°), and Reynolds number (Re = 1,300–1800) on the flow and heat transfer characteristics of working fluid. It was found that the heat transfer capacity of nanofluids increases with nanofluids mass fraction, angular frequency, amplitude and phase shift. Finally, the comprehensive performance of the whole experimental system was studied, and it was found that the greater the angular frequency, amplitude and phase shift, the better the comprehensive performance of the heat exchanger.