Natural dietary compound naringin inhibits glioblastoma cancer neoangiogenesis

Abstract

Background: Flavonoids, which existed nearly in all fruits and vegetables, are considered as a class of plantsecondary metabolites with a polyphenolic structure and have properties with health-improving potential. Yet, not so many experimental focus on the benefits of flavonoid in vivo after external application. Here we assessed the impacts of naringin in vitro and in vivo in the human glioma U-87 cells implanted into athymic mice. Methods: Tumor size and animal survival time were followed in naringin-treated mice bearing subcutaneous gliomas. To define the effects of naringin on angiogenesis, in vitro, tube formation and migration were assayed using endothelial HUVEC cell line. Results: Low concentration of naringin remarkably inhibited tubulogenesis and reduced cell invasion. Moreover, naringin has been shown to have a toxicity effect on U-87 cells in a dose-dependent way. Furthermore, naringin administration (120 mg/kg/day) applies serious anti-cancer belongings on glioblastoma, as demonstrated by a slow cancer progression. Conclusions: Our study has provided the first evidence on the antitumor effect of naringin, which is somehow due to the inhibition of invasion and angiogenesis.

Neuroimmunomodulatory Properties of Flavonoids and Derivates: A Potential Action as Adjuvants for the Treatment of Glioblastoma

“…For example, Guo et al [57] demonstrated that Ampelopsin inhibited tumor growth and progression in mouse xenograft models, and induced apoptotic response via both intrinsic and extrinsic signaling pathways. Aroui et al [58] showed that narigenin inhibited invasion and angiogenesis in an in vivo model of subcutaneous glioma. Further, in a prospective study of dietary flavonoid intake and risk of glioma in US citizens, Bever et al [59] concluded that increased dietary intakes of flavan-3-ol and polymeric flavonoids were associated with decreased risks of developing this brain tumor, without compromising the functions of other CNS cells.…”

A Systematic Review of the Preventive and Therapeutic Effects of Naringin Against Human Malignancies

“…Results indicated that naringin had a toxic impact on the U-87 cell line and reduced cancer cell proliferation and viability in a concentration-dependent way. Moreover, naringin administration also suppressed tubulogenesis and angiogenesis and reduced tumor size and cell invasion U-87 mouse xenograft tumor model ( Table 4 ) ( Aroui et al, 2020 ). Another study demonstrated that naringin could specifically suppress the focal adhesion kinase (FAK) activity and inhibit the FAKp-Try397 and its downstream pathway in glioblastoma cells.…”

Natural Compounds in Glioblastoma Therapy: Preclinical Insights, Mechanistic Pathways, and Outlook

“…Reduced VEGF activity decreases tumor neovascularization; importantly, this is observable in vivo. Treatment of U87 xenografts in athymic mice with the flavonoid naringin downregulates tumor hemoglobin and the angiogenic markers cluster of differentiation 31 (CD31) and 105 (CD105) [ 37 ]. Moreover, matteucinol decreases the angiogenic area and the number of blood vessel junctions in a U251 xenograft-fertilized chicken egg model [ 36 ].…”