Natural enamel caries in quinoline: Volumetric data and the pattern of infiltration

Brito, Camila Santos de Mattos; Meira, Kássia Regina Simões; Sousa, Frederico Barbosa de

doi:10.1002/jemt.22962

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…Consistently with previous reports on the infiltration of aqueous solutions [Meira et al, 2015] and oil (quinoline) [de Mattos Brito et al, 2018] in human NEC and resin infiltrant in human fluorotic enamel [De Sousa et al, 2017], subsurface lesions presented a small fraction of the pore volume available for infiltration, and the resin-occluded pore consisted in the sum of resin, air, firmly bound water, and organic matter. Validation of resin infiltration with confocal laser scanning microscopy exploring the autofluorescence of the resin infiltrant (Fig.…”

Section: Discussionsupporting

confidence: 76%

High Amount of Organic Matter during Caries Formation Reduces Remineralization and Resin Infiltration of Enamel Caries

et al. 2018

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The amount of organic material in the cariogenic environment correlates with the amount of organic material incorporated in carious enamel. The incorporated organic material may be expected to reduce the pore volumes available for remineralization and resin infiltration, but these expected outcomes have not yet been quantified. We tested the effect of the amount of organic content in the cariogenic agent on remineralization and the resin-occluded pore volume in artificial subsurface enamel caries. An acid gel (organic-rich; G1) and an aqueous solution (organic-poor; G2) were used to induce subsurface lesions in human enamel. Undemineralized histological sections were prepared, microradiographed, and then submitted to resin infiltration in vitro. The enamel component volumes (mineral, organic, remineralizable [total water volume], loosely and firmly bound water volumes, and resin-occluded volume) were measured (by microradiography and polarizing microscopy) at histological sites (n = 38, G1; n = 34, G2). The main outcomes were the differences between the experimental and the predicted volumes (Δremineralizable and Δresin-occluded volumes). Resin infiltration was confirmed by confocal scanning laser microscopy. Compared to G2, G1 presented more incorporated organic volume and lower Δremineralizable volume (p = 0.003; Hedges g = 0.66; power = 0.87), a lower increase in loosely bound water volume (p = 0.0013; Hedges g = 0.74; power = 0.93), a lower remineralization volume in the surface layer (p = 0.017; Hedges g = 0.68; power = 0.8), and a lower Δresin-occluded volume (p = 0.0015; Hedges g = 0.73; power = 0.92). In conclusion, the higher amount of organic matter in the cariogenic gel negatively affected remineralization and the resin-occluded volume in subsurface lesions.

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Section: Discussionsupporting

confidence: 76%

High Amount of Organic Matter during Caries Formation Reduces Remineralization and Resin Infiltration of Enamel Caries

et al. 2018

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“…A detailed description of enamel structure in 2D and 3D using several imaging techniques has recently been reported for human carious enamel, showing the complex demineralised structures [ 39 ]. Different regions have been traditionally reported, using optical microscopy and radiography, and referred to as ‘body of the lesion’, ‘surface’, ‘translucent’, and ‘dark’ zones of enamel caries [ 135 , 174 , 180 , 181 , 182 ] ( Figure 2 d); however, the presence of additional zones should not to be omitted [ 39 , 183 ]. The surface zone is a critical region of enamel (in direct contact with the environment and dental plaque), which has not yet been totally understood and characterised.…”

Section: Demineralisation and Remineralisation Of Teethmentioning

confidence: 99%

Synchrotron X-ray Studies of the Structural and Functional Hierarchies in Mineralised Human Dental Enamel: A State-of-the-Art Review

et al. 2023

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Hard dental tissues possess a complex hierarchical structure that is particularly evident in enamel, the most mineralised substance in the human body. Its complex and interlinked organisation at the Ångstrom (crystal lattice), nano-, micro-, and macro-scales is the result of evolutionary optimisation for mechanical and functional performance: hardness and stiffness, fracture toughness, thermal, and chemical resistance. Understanding the physical–chemical–structural relationships at each scale requires the application of appropriately sensitive and resolving probes. Synchrotron X-ray techniques offer the possibility to progress significantly beyond the capabilities of conventional laboratory instruments, i.e., X-ray diffractometers, and electron and atomic force microscopes. The last few decades have witnessed the accumulation of results obtained from X-ray scattering (diffraction), spectroscopy (including polarisation analysis), and imaging (including ptychography and tomography). The current article presents a multi-disciplinary review of nearly 40 years of discoveries and advancements, primarily pertaining to the study of enamel and its demineralisation (caries), but also linked to the investigations of other mineralised tissues such as dentine, bone, etc. The modelling approaches informed by these observations are also overviewed. The strategic aim of the present review was to identify and evaluate prospective avenues for analysing dental tissues and developing treatments and prophylaxis for improved dental health.

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“…Back then, carious lesions in enamel were often examined by using different immersion mediums to display different zones of enamel caries [ 18 ]. However, early carious lesions are still examined by using immersion mediums like quinoline in order to measure the actual depth of the enamel caries and to obtain conclusions about the pore volume [ 17 , 19 ]. Additionally, different approaches have been made to investigate the ultrastructure of dentin with polarized light microscopy.…”

Section: Introductionmentioning

confidence: 99%

Visualization of carious lesions with polarized and depolarized light microscopy

Grundmann,

Golde,

Steuer

et al. 2024

Biomed. Opt. Express

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Polarized light microscopy (PLM) is an established method in dental histology for investigating the ultrastructure and carious process of teeth. This study introduces a novel approach for measuring the degree of polarization (DOP) in a modified PLM setup and uses the DOP to assess the changes of the optical properties of enamel and dentin due to caries. The validation is provided by a comparison with complementary imaging methods, i.e. standard PLM and µCT. The results show that demineralization is reliably displayed by the DOP in accordance with the common imaging methods, and that this quantitative analysis of depolarization allows the characterization of the different pathohistological zones of caries.

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Natural enamel caries in quinoline: Volumetric data and the pattern of infiltration

Cited by 3 publications

References 24 publications

High Amount of Organic Matter during Caries Formation Reduces Remineralization and Resin Infiltration of Enamel Caries

High Amount of Organic Matter during Caries Formation Reduces Remineralization and Resin Infiltration of Enamel Caries

Synchrotron X-ray Studies of the Structural and Functional Hierarchies in Mineralised Human Dental Enamel: A State-of-the-Art Review

Visualization of carious lesions with polarized and depolarized light microscopy

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