Research on the contribution of understory components to the total above ground biomass (AGB) has to date received very little attention because most prior biomass estimation studies have ignored small regenerating trees beneath the main canopy with the assumption that their contribution to biomass is generally negligible. Only a few biomass studies have emphasized a considerable contribution to biomass of understory components in forest ecosystems. However, this study of native, tropical, deciduous forest biomass in the Central Highlands of Vietnam was able to explore the contribution of small regenerating trees to total biomass by exploiting a large field inventory of hundreds to thousands of individually-counted small regenerating trees per hectare. Thus, this study investigated the influence of small regenerating tree biomass on models of the relationship between total AGB and remote sensing data. These analyses were trained with and without topographic variables derived from ASTER-GDEM. Our results demonstrate that the inclusion of small regenerating understory trees (R2 = 0.42, NRMSE or %RMSE = 30.5%) provides a quantifiable improvement in total estimated AGB compared to using only large woody canopy trees (R2 = 0.21, NRMSE or %RMSE = 36.6%) when correlating field-based biomass measurements with optical image-derived variables. All analyses show that the inclusion of terrain factors made an important contribution to biomass modeling. This study suggests that for young, open forests where there are many small regenerating trees, the contribution of understory biomass should be taken into consideration to improve total AGB estimation.