Natural Killer Cells Differentiate Human Adipose-Derived Stem Cells and Modulate Their Adipogenic Potential

Rezzadeh, Kameron; Jewett, Anahid; Kozłowska, Anna; Segovia, Luis A.; Zuk, Patricia A.; Jarrahy, Reza

doi:10.1097/prs.0000000000001536

Cited by 4 publications

(1 citation statement)

References 33 publications

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“…From this perspective, IL-15 and these other adipocyte-derived factors appear to support tumor growth through both direct effects and also increased tumor vascularization. MSC adipogenic differentiation has also been found to be modulated by natural killer cells ( 165 ), suggesting that a forward feedback loop between inflammation and adipogenesis may be at work. These data suggest that adipocytes not only are affected by, but also affect, the immune system.…”

Section: Linking Bmat and Systemic Inflammationmentioning

confidence: 99%

Signaling Interplay between Bone Marrow Adipose Tissue and Multiple Myeloma cells

2016

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In the year 2000, Hanahan and Weinberg (1) defined the six Hallmarks of Cancer as: self-sufficiency in growth signals, evasion of apoptosis, insensitivity to antigrowth mechanisms, tissue invasion and metastasis, limitless replicative potential, and sustained angiogenesis. Eleven years later, two new Hallmarks were added to the list (avoiding immune destruction and reprograming energy metabolism) and two new tumor characteristics (tumor-promoting inflammation and genome instability and mutation) (2). In multiple myeloma (MM), a destructive cancer of the plasma cell that grows predominantly in the bone marrow (BM), it is clear that all these hallmarks and characteristics are in play, contributing to tumor initiation, drug resistance, disease progression, and relapse. Bone marrow adipose tissue (BMAT) is a newly recognized contributor to MM oncogenesis and disease progression, potentially affecting MM cell metabolism, immune action, inflammation, and influences on angiogenesis. In this review, we discuss the confirmed and hypothetical contributions of BMAT to MM development and disease progression. BMAT has been understudied due to technical challenges and a previous lack of appreciation for the endocrine function of this tissue. In this review, we define the dynamic, responsive, metabolically active BM adipocyte. We then describe how BMAT influences MM in terms of: lipids/metabolism, hypoxia/angiogenesis, paracrine or endocrine signaling, and bone disease. We then discuss the connection between BMAT and systemic inflammation and potential treatments to inhibit the feedback loops between BM adipocytes and MM cells that support MM progression. We aim for researchers to use this review to guide and help prioritize their experiments to develop better treatments or a cure for cancers, such as MM, that associate with and may depend on BMAT.

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Section: Linking Bmat and Systemic Inflammationmentioning

confidence: 99%

Signaling Interplay between Bone Marrow Adipose Tissue and Multiple Myeloma cells

2016

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Characterization of two distinct lipomas: a comparative analysis from surgical perspective

Chang

Park

Jin

et al. 2017

Journal of Plastic Surgery and Hand Surgery

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Adipose-Derived Stem Cells and Ceiling Culture-Derived Preadipocytes Cultured from Subcutaneous Fat Tissue Differ in Their Epigenetic Characteristics and Osteogenic Potential

Sasahara

Kubota

Kosaka

et al. 2019

Plastic &Amp; Reconstructive Surgery

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Background: Adipose-derived stem cells and ceiling culture-derived preadipocytes can be harvested from subcutaneous adipose tissue. Little is known about the epigenetic differences, which may contribute to differences in osteogenic potential, between these cell types. The purpose of this study was to address the osteogenic potential and underlying epigenetic status of adipose-derived stem cells and ceiling culture-derived preadipocytes. Methods: Adipose-derived stem cells and ceiling culture-derived preadipocytes were cultured from abdominal subcutaneous fat tissues of four metabolically healthy, lean female patients. After 7 weeks of culture, cellular responses to osteogenic differentiation media were examined. To evaluate the osteogenic potentials of undifferentiated adipose-derived stem cells and ceiling culture-derived preadipocytes, two types of epigenetic assessment were performed using next-generation sequencing: DNA methylation assays with the Human Methylation 450K BeadChip, and chromatin immunoprecipitation assays for trimethylation of histone H3 at lysine 4. Results: Human ceiling culture-derived preadipocytes showed greater osteogenic differentiation ability than did adipose-derived stem cells. In an epigenetic survey of the promoters of four osteogenic regulator genes (RUNX2, SP7, ATF4, and BGLAP), the authors found a general trend toward decreased CpG methylation and increased trimethylation of histone H3 at lysine 4 levels in ceiling culture-derived preadipocytes as compared to adipose-derived stem cells, indicating that these genes were more likely to be highly expressed in ceiling culture-derived preadipocytes. Conclusions: The surveyed epigenetic differences between adipose-derived stem cells and ceiling culture-derived preadipocytes were consistent with the observed differences in osteogenic potential. These results enhance the authors’ understanding of these cells and will facilitate their further application in regenerative medicine.

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Natural Killer Cells Differentiate Human Adipose-Derived Stem Cells and Modulate Their Adipogenic Potential

Abstract: The authors demonstrate that, through secreted factors, natural killer cells are capable of differentiating adipose-derived stem cells. In cells maintained in adipogenic media, treatment with natural killer cell supernatant modulated adipogenic potential.

Cited by 4 publications

References 33 publications

Signaling Interplay between Bone Marrow Adipose Tissue and Multiple Myeloma cells

Signaling Interplay between Bone Marrow Adipose Tissue and Multiple Myeloma cells

Characterization of two distinct lipomas: a comparative analysis from surgical perspective

Adipose-Derived Stem Cells and Ceiling Culture-Derived Preadipocytes Cultured from Subcutaneous Fat Tissue Differ in Their Epigenetic Characteristics and Osteogenic Potential

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