Natural Occurrence of Wild tomato mosaic virus in Wild Eggplant in China

Abstract

In winter 2013, typical symptoms of green crinkling and mosaic were observed on wild eggplant leaves in field in Hainan, China. The causal pathogen was identified to be a Wild tomato mosaic virus (WTMV) based upon reverse transcription‐touchdown PCR with degenerate primers. A specific product of 1700 bp containing the partial sequence of the NIb (~950 bp) and CP (~750 bp) coding regions of WTMV was amplified, and the predicted polypeptide consisted of 572 amino acids (Mr 65.52 kDa). This isolate was subsequently named WTMV‐Hainan (GenBank accession ) isolate, and it shared sequence identities of 91% and 95% with WTMV‐Laichau isolate at the nucleotide and the amino acid levels, respectively. Phylogenetic analysis of the CP gene sequences provided further evidence that all WTMV isolates formed a high‐confidence subclade and WTMV‐Hainan most closely related to WTMV‐KAN, WTMV‐Laichau and WTMV‐GD1. To our knowledge, this is the first report of the natural infection of WTMV on wild eggplant in China.

The Characterization of the Tobacco-Derived Wild Tomato Mosaic Virus by Employing Its Infectious DNA Clone

“…Wild tomato mosaic virus (WTMV) is a potyvirus member that was initially identified in wild eggplants ( Solanum torvum , called wild tomato in the original publication) collected in Laichau of Vietnam from field surveys in the early 2000s [ 18 ]. Later, it was found in S. torvum , black nightshade ( Solanum nigrum ), C. annuum plants, and tobaccos in various locations in China [ 19 , 20 , 21 ]. WTMV was firstly reported to infect tobacco grown in mid-May 2013 in Nanxiong (Guangdong Province, China), where yellow sun-cured tobacco has been produced for more than 300 years [ 22 ].…”

Molecular characterization of wild tomato mosaic virus and chilli veinal mottle virus mix‐infecting chilli pepper in China

“…Wild tomato mosaic virus (WTMV) also belongs to the genus Potyvirus and was first identified in wild tomatoes in Vietnam in 2008 (Ha et al, 2008). Furthermore, it has been detected in various crops, including tobacco, wild eggplant, and Solanum nigrum L., in Guangdong, Hainan, and Sichuan regions of China (Du et al, 2014; Sun et al, 2016; Zhang et al, 2014; Zhang et al, 2019). Presently, only three complete genome sequences have been reported, including isolates from wild tomato, tobacco, and Solanum nigrum L.…”

Molecular Characterization of Two Isolates of Wild Tomato Mosaic Virus and Chilli Veinal Mottle Virus Co-infecting Chilli Pepper in China

“…Wild tomato mosaic virus (WTMV), also a member of the genus Potyvirus, was rst shown to infect wild tomatoes in Vietnam in 2008 [3]. Later, the disease was reported to infect various crops, including tobacco, wild eggplant, and Solanumnigrum L. in Guangdong, Hainan and Sichuan regions of China [11][12][13][14]. At present, however, only three complete genome sequences have been reported, including isolates from wild tomato, tobacco, and Solanumnigrum L.…”

“…This polyprotein is cleaved into 10 functional proteins as described previously [3][4][5].Chili veinal mottle virus (ChiVMV), a member of the genus Potyvirus, was rst reported in West Malaysia in 1979 [6] after which it has been widely reported in many Asian and East African nations as an important pathogen of chilli crops [7-10].Wild tomato mosaic virus (WTMV), also a member of the genus Potyvirus, was rst shown to infect wild tomatoes in Vietnam in 2008 [3]. Later, the disease was reported to infect various crops, including tobacco, wild eggplant, and Solanumnigrum L. in Guangdong, Hainan and Sichuan regions of China [11][12][13][14]. At present, however, only three complete genome sequences have been reported, including isolates from wild tomato, tobacco, and Solanumnigrum L.In July 2018, a chilli pepper sample that showed virus-like symptoms, including mosaicism, yellow mottling and shrinkage (Fig.…”