Natural Polymer-Based Sulfite Biosensor

Abstract

As a natural product, chitosan has the inherent properties of being biodegradable, biocompatible and nontoxic. These properties render it an ideal matrix in enzyme immobilization for clinical analysis. In this work, the feasibility of electrochemical biosensor fabrication using chitosan has been demonstrated. As a test case, the enzyme, sulfite oxidase (SOD), was covalently immobilized onto the matrix of chitosan-poly(hydroxyethyl methacrylate) (chitosan-HEMA), a natural/synthetic polymer hybrid material obtainable via a UV curing process. An electron transfer mediator, pbenzoquinone was coupled onto the polymer network for the activation of chitosan-HEMA copolymer after the photo-induced polymerization reaction. The biological activity of the immobilized SOD and the electroactivity of the coupled p-benzoquinone were examined.

Development of semi-interpenetrating carbohydrate polymeric hydrogels embedded silver nanoparticles and its facile studies on E. coli

“…Poly(hydroxyethyl methacrylate) (PHEMA) has good biocompatibility and mechanical strength required for biomedical applications. PHEMA is a hydrogel that swells but is insoluble in water and hence, it possesses the ability to retain water within its structure (Ng, Yuan, & Zhao, 1998;Sastre, Blanco, Gomez, Socorro, & Teijon, 1999).…”

Electrochemically deposited nanocomposite film of CS-Fc/Au NPs/GOx for glucose biosensor application

“…Chitosan (CS), a polysaccharide biopolymer, displays a remarkable combination of physicochemical properties including an excellent membrane-forming ability, good biocompatibility, high permeability towards water, good adhesion, non-toxicity, and high mechanical strength. These properties make CS a promising matrix for enzyme immobilization (Ng et al, 1998;Wang et al, 2003;Cruz et al, 2000;Qian and Yang, 2006;Wang et al, 2005b). Moreover, a large number of CS's hydroxyl and amino groups are reaction sites for chemical modifications and suitable for anchoring a large variety of organometallic complexes, which makes CS a good candidate as a precursor for molecular immobilization (Vincent and Guibal, 2003;Alonso-Sande et al, 2006;Abd-El-Aziz, 2002).…”

Construção e aplicação de biossensores usando diferentes procedimentos de imobilização da peroxidase de vegetal em matriz de quitosana

“…Comprovaram que a enzima apresentou estabilidade superior quando comparada com a lacaselivre. A quitosana, quimicamente modificada, tem sido empregada tanto na forma de microesferas, quanto na de filmes, ou de pó, ou de membranas, como adsorvente de metais 10,[15][16][17] , na imobilização de diversas enzimas (creatinase deaminase 19 , galactose oxidase 20 , glutamato oxidase 20 , glicose oxidase 21 , peroxidase [22][23][24] , lactato oxidase 25 , sulfito oxidase 26,27 ) e na construção de biossensores [19][20][21][22][23][24][25][26][27] . A enzima peroxidase pode ser encontrada em vários vegetais e catalisa a oxidação de mono, di e polifenóis em presença de peróxido de hidrogênio 28,29 .…”

“…Comprovaram que a enzima apresentou estabilidade superior quando comparada com a lacaselivre. A quitosana, quimicamente modificada, tem sido empregada tanto na forma de microesferas, quanto na de filmes, ou de pó, ou de membranas, como adsorvente de metais 10,[15][16][17] 26,27 ) e na construção de biossensores [19][20][21][22][23][24][25][26][27] . …”