Natural Polymeric Film Encapsulating Propolis Nano-Formulation for Cutaneous Wound Healing

Abstract

The present paper describe the production and characterization of novel collagen films containing propolis encapsulated in chitosan nanoparticles, for biomedical applications such as cutaneous wound healing. Structural and morphological details were investigated by ATR FTIR spectroscopy, SEM and nanoindentation measurements, revealing the collagen fibers aligned in a quasi-parallel distribution, which might be favorable for biomedical applications. Moreover, the vibrational marker bands of propolis were well preserved in the final polymeric mixture, indicating the stability of bioactive compounds upon the encapsulation procedure. The antibacterial effect depends on the nanoparticles concentration in collagen film, the effect being more evident with respect to E. coli than S. aureus. The antioxidant capacity monitored by CUPRAC assay, indicated a synergic effect of chitosan nanoparticles matrix and propolis extract, incorporated in collagen films.

Nanomaterials Based on Honey and Propolis for Wound Healing—A Mini-Review

“…In relation to the thermal stability of propolis dressings, results agreed that the incorporation into PU matrices decreases orientation of the polymer chains and crystallinity [94,100]. However, the thermal stability of the as-formed cellulose acetate (CA)/propolis marginally increases when compared with CA nanofibers [97].…”

“…Tensile strength and elongation at maximum stress are two important mechanical properties that determine the stretchability of a wound dressing [66]. In general, results show an improvement in dressing elasticity due to the addition of honey [66,67,75,94] Z. Rafati et al reported that tensile strength and elongation at maximum stress in groups treated with honey-loaded bionanocomposite hydrogel wound dressings were comparable, and in most cases higher, than the control group due to better collagenization [66]. The elastic behavior of poly(1,4-cyclohexane dimethylene isosorbide terephthalate (PICT)/honey nanofibers was improved by increasing the concentration of honey in the polymer solution, but this increase in concentration also caused a decrease in the Young's modulus [76].…”

Honey and propolis for wound healing

“…48 Propolis nanoparticles have anti-inflammatory and antiapoptotic effects as decreased expression of TGF-β and increased the expression of Bcl-2 in liver and kidney. 101 Propolis containing nanohydroxyapatite (the main inorganic component in natural bone) was able to hinder the bacterial growth and biofilm formation, as well as be non-cytotoxic to fibroblast cells. 102 For possible cutaneous wound healing applications, there is found synergic effect of chitosan nanoparticles matrix and propolis extract, incorporated in collagen films.…”

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“…The interaction between extra cellular matrix (ECM) proteins and different cells triggers the inflammatory response and phagocytosis stimulated by foreign bodies or damaged tissue present in the wound site. It is well known that platelets activation, cytokines secretion, macrophages, fibroblasts and keratinocytes are the most important active factors to promote wound closure and formation of new tissue [4][5][6].…”