Natural product-based nanomedicines for wound healing purposes: therapeutic targets and drug delivery systems

Goorani

et al. 2019

Applied Organom Chemis

117

100

The exploitation of various plant materials for the biosynthesis of nanoparticles is considered a green technology because it does not involve any harmful chemicals. The aim of the experiment was chemical characterization and evaluation of cytotoxicity, antioxidant, antibacterial, antifungal, and cutaneous wound healing activities of gold nanoparticles using aqueous extract of Gundelia tournefortii L. leaves (AuNPs@GT). These nanoparticles were characterized by fourier transformed infrared spectroscopy (FT‐IR), field emission scanning electron microscopy (FE‐SEM), energy dispersive X‐ray spectroscopy (EDS), and UV–visible spectroscopy. DPPH free radical scavenging test was done to assess the antioxidant properties, which indicated similar antioxidant potentials for AuNPs@GT and butylated hydroxytoluene. Agar diffusion tests were applied to determine the antibacterial and antifungal characteristics. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Minimum Fungicidal Concentration (MFC) were specified by macro‐broth dilution assay. AuNPs@GT indicated higher antibacterial and antifungal effects than all standard antibiotics (p ≤ 0.01). Also, AuNPs@GT inhibited the growth of all bacteria and fungi and removed them at 2‐4 mg/mL concentrations (p ≤ 0.01). In vivo experiment, after creating the cutaneous wound, the rats were randomly divided into six groups: untreated control, treatment with Eucerin basal ointment, treatment with 3% tetracycline ointment, treatment with 0.2% HAuCl4 ointment, treatment with 0.2% G. tournefortii ointment, and treatment with 0.2% AuNPs@GT ointment. These groups were treated for 10 days. For histopathological and biochemical analysis of the healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses at day 10. Use of AuNPs@GT ointment in the treatment groups substantially reduced (p ≤ 0.01) the wound area, total cells, neutrophil, and lymphocyte and remarkably raised (p ≤ 0.01) the wound contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, fibroblast, and fibrocytes/fibroblast rate compared to other groups. The synthesized AuNPs@GT had great cell viability dose‐dependently (Investigating the effect of the plant on HUVEC cell line) and revealed this method was nontoxic. The results showed that the leave aqueous extract of G. tournefortii is very good bioreductant in the synthesis of gold nanoparticles for treatment of bacterial, fungal, and skin diseases.

Section: Introductionmentioning

confidence: 99%

Section: Introductionunclassified

In vitro and in vivo evaluation of cytotoxicity, antioxidant, antibacterial, antifungal, and cutaneous wound healing properties of gold nanoparticles produced via a green chemistry synthesis using Gundelia tournefortii L. as a capping and reducing agent

Zhaleh

Goorani

et al. 2019

Applied Organom Chemis

117

100

“…In recent years, use of chemicals has encouraged the researchers to conduct many studies on the use of traditional and herbal medicines. These studies have revealed that natural drugs are the only treatment modality in some cases, and the compounds available in them have been used in pharmaceutical industries . Because no definitive drug has been introduced for wound healing, it is necessary to perform studies on the effects of herbal drugs and their metal nanoparticles (NPs) on cutaneous wound healing .…”

Section: Introductionmentioning

confidence: 99%

“…These studies have revealed that natural drugs are the only treatment modality in some cases, and the compounds available in them have been used in pharmaceutical industries . Because no definitive drug has been introduced for wound healing, it is necessary to perform studies on the effects of herbal drugs and their metal nanoparticles (NPs) on cutaneous wound healing . A list of plants that are synthesized by metal NPs for increasing the cutaneous wound‐healing effects includes Piper nigrum , Phytophthora infestans , Terminalia chebula Retz., Orchidantha chinensis T.L.Wu, Naringi crenulata (Roxb.)…”

Section: Introductionmentioning

confidence: 99%

“…Nicolson, Nyctanthes arbor‐tristis L., Trigonella foenum‐graecum , Melia dubia Cav., Momordica charantia L., Indigofera aspalathoides DC., Lansium domesticum Corrêa, Ficus religiosa , Fraxinus angustifolia , Eucalyptus globulus , Drosera binata Labill., Curcuma longa L., Cymbopogon citratus (DC.) Stapf, Cassia auriculata L., Cassia roxburghii , Carica papaya L., Biophytum sensitivum , Bryonia laciniosa L., Arnebia nobilis Rech.f., Azadirachta indica A., Aloe vera , Centella asiatica , Coleus forskohlii , Chromola enaodorata and Citrus reticulate …”

Section: Introductionunclassified

See 1 more Smart Citation

Green synthesis of silver nanoparticles using aqueous extract of Stachys lavandulifolia flower, and their cytotoxicity, antioxidant, antibacterial and cutaneous wound‐healing properties

Joshani

et al. 2019

Applied Organom Chemis

In a biological process where the herbal tea (Stachys lavandulifolia) aqueous extract was applied as a capping and reducing agent, nanoparticles (NPs) of silver (Ag) were synthesized. These AgNPs were characterized using Fourier transform‐infrared spectroscopy, field emission‐scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, transmission electron microscopy and ultraviolet–visible spectroscopy. The synthesized AgNPs had great cell viability dose‐dependently [investigating the effect of the plant on human umbilical vein endothelial cell line] and indicated this method was non‐toxic. In this study, the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical scavenging test was carried out to examine antioxidant properties, which revealed similar antioxidant properties for AgNPs and butylated hydroxytoluene. Agar diffusion tests were applied to determine the antibacterial characteristics. The macro‐broth tube test was run to determine minimum inhibitory concentration. All data of antibacterial and cutaneous wound‐healing examinations were analyzed by SPSS 21 software (Duncan post hoc test). AgNPs showed higher antibacterial property than all standard antibiotics (p ≤ 0.01). Also, AgNPs prevented the growth of all bacteria at 2–8 mg/ml concentrations and destroyed them at 2–16 mg/ml concentrations (p ≤ 0.01). For the in vivo experiment, after creating the cutaneous wound, the rats were randomly divided into six groups: untreated control; treatment with Eucerin basal ointment; treatment with 3% tetracycline ointment; treatment with 0.2% AgNO3 ointment; treatment with 0.2% S. lavandulifolia ointment; and treatment with 0.2% AgNPs ointment. These groups were treated for 10 days. For histopathological and biochemical analysis of the healing trend, a 3 × 3‐cm section was prepared from all dermal thicknesses at day 10. Use of AgNPs ointment in the treatment groups substantially reduced (p ≤ 0.01) the wound area, total cells, neutrophil, macrophage and lymphocyte, and remarkably raised (p ≤ 0.01) the wound contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte and fibrocytes/fibroblast rate compared with other groups. Seemingly, AgNPs can be used as a medical supplement owing to their non‐cytotoxic, antioxidant, antibacterial and cutaneous wound‐healing properties.

Encyclopedia of Polymer Science and Technology

Polymer Nanofibers

Mandal

Song

Zeng

et al. 2023

The aim of this article is to provide an overview of nanofibers' properties, functionality enhancement, and applications. In order to achieve this, the significant properties of nanofibers were first demonstrated. Based on this demonstration, it was concluded that nanofibers have potential use for a wide range of applications. Additionally, the functionality enhancements of nanofibers through their physical and chemical modifications were reviewed. This functionality enhancement process helps to effectively apply the nanofibers in the engineering and medical fields. The applications of nanofibers in different hi‐tech engineering and medical fields were described. This article identified various gaps and limitations associated with nanofibers' applications and suggested further research directions. Based on this article, it has been found that nanofibers could be produced with several properties and structures including shape, size, and strength. As a result, nanofibers could be applicable in high‐tech engineering and medical sectors such as tissue engineering, sensors, and enzyme carrier. As nanofiber is a porous media, the applicability of nanofibers could be widened in different fields like protective apparel, lightweight military products, and so on. This article can help polymer engineers to improve and extend the applicability of nanofibers in evolving engineering and medical fields. In turn, this article could help to advance the field of high‐tech engineering and medical field for better human life.