Natural product extracts of the Canadian prairie plant,<i>Thermopsis rhombifolia,</i>have anti-cancer activity in phenotypic cell-based assays

Swift, Lucy; Lewis, Cody W.; Bruyère, Céline; Oumata, Nassima; Colas, Pierre; Ruchaud, Sandrine; Bain, John; Golsteyn, Roy M.

doi:10.1080/14786419.2014.979423

Cited by 9 publications

(9 citation statements)

References 16 publications

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“…Utilization of the native legume Thermopsis rhombifolia (Nutt. ex Pursh) Richardson is also being investigated for medical purposes such as anticancer effects in humans (Kerneis et al 2015). The relevance of native perennial bromegrass as forage has been emphasized (Cayouette et al 1997).…”

Section: National Germplasm Collectionsmentioning

confidence: 99%

Genetic Resources of Crop Wild Relatives: A Canadian Perspective

Diederichsen

Schellenberg

2018

North American Crop Wild Relatives, Volume 1

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Canada is home to about 5087 species of higher plants of which 25% were introduced to Canada either deliberately or by accident. The richness of botanical species is highest in the southern, more densely settled parts of the country. About 364 native Canadian species have direct or potential use in crop development for various usages with particular emphasis on use for berries and as forages. The use of more than 600 plant species by indigenous people for food, medicine, or spiritual reasons is documented, and they practiced agriculture prior to colonization by Europeans with cultivations of corn, garden bean, squash, sunflower, and tobacco. Only a few native species are crop wild relatives of major agricultural crops such as 14 species of the genus Helianthus L., which are related to cultivated sunflower. Taxonomists have made major contributions to recognize the potential of the Canadian flora from a utilitarian aspect. Plant breeding in Canada has been done by the government of Canada, by universities, and, more recently, also by the private sector. The focus has been on major crops, while the activities in forages and minor crops have decreased. Canada maintains a national genebank for ex situ conservation (Plant Gene Resources of Canada). More activities to enhance the complementarity between in situ and ex situ conservation should be undertaken in Canada. The potential of native rangeland plants or weeds as genetic resources is emphasized. The "integrating genebank" is suggested as an ambitious concept. In order to maintain the future options Canadian biodiversity holds for enhancing life and livelihoods, a national strategy is required.

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Section: National Germplasm Collectionsmentioning

confidence: 99%

Genetic Resources of Crop Wild Relatives: A Canadian Perspective

Diederichsen

Schellenberg

2018

North American Crop Wild Relatives, Volume 1

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“…U2OS cells were either not treated or treated with a range of concentrations of PP-630F, then exposed to ambient light and observed by phasecontrast microscopy. In parallel, U2OS cells were treated with extract PP-120, prepared in a similar manner as that of PP-630F from a previously characterized prairie plant species, Thermopsis rhombifolia [3] (Figure 4A). Not-treated cells and PP-120-treated cells did not acquire vacuoles, as expected, whereas cells treated with PP-630F had light-induced vacuoles in a dose dependent manner.…”

Section: Resultsmentioning

confidence: 99%

“…We are investigating plants from the prairie ecological zone to discover new cell biology tools with which to study how human cells function. We have previously reported that extracts prepared from several prairie plant species, including Thermopsis rhombifolia that induced a cell cycle arrest identified by phenotypic assays [3,9]. Here, we identified extracts prepared from Symphoricarpos occidentalis (Caprifoliaceae) that induce a striking cellular phenotype when applied to human cells.…”

Section: Introductionmentioning

confidence: 89%

“…Canada harbours 15 terrestrial ecozones including the prairie zone, which has one of the highest levels of biodiversity in temperate northern latitudes [1]. Despite extensive research into plant natural products worldwide, the prairie ecological zones are rarely investigated for plants with bioactive secondary metabolites [2][3][4]. Prairie plant species have coevolved with both abiotic and biotic stresses, such as the prevalence of snowpacks that shorten the growing season, and the presence of grazing herbivores [5].…”

Section: Introductionmentioning

confidence: 99%

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Extracts Prepared from a Canadian Toxic Plant Induce Light-Dependent Perinuclear Vacuoles in Human Cells

Tuescher

Beck

Spencer

et al. 2021

Toxins

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We are investigating plant species from the Canadian prairie ecological zone by phenotypic cell assays to discover toxins of biological interest. We provide the first report of the effects of extracts prepared from the shrub Symphoricarpos occidentalis in several human cell lines. S. occidentalis (Caprifoliaceae) extracts are cytotoxic, and, strikingly, treated cells undergo light-dependent vacuolation near the nucleus. The range of irradiation is present in standard ambient light and lies in the visible range (400-700 nm). Vacuolization in treated cells can be induced with specific wavelengths of 408 or 660 nm at 1 J/cm2 energies. Vacuolated cells show a striking phenotype of a large perinuclear vacuole (nuclear associated vacuole, NAV) that is distinct from vesicles observed by treatment with an autophagy-inducing agent. Treatment with S. occidentalis extracts and light induces an intense lamin A/C signal at the junction of a nuclear vacuole and the nucleus. Further study of S. occidentalis extracts and vacuolation provide chemical tools that may contribute to the understanding of nuclear envelope organization and human cell biology.

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“…We have previously reported that extracts prepared from the prairie plant species, T. rhombifolia, were toxic to human cells and induced a cell cycle arrest [10].…”

Section: Introductionmentioning

confidence: 99%

The Canadian Prairie Plant Thermopsis rhombifolia Contains Luteolin, a Flavone that Inhibits Cyclin Dependent Kinase 9 and Arrest Cells in the G1-Phase of the Cell Cycle

Tuescher¹,

Tailfeathers²,

Baratte³

et al. 2020

J NHP Res

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INTRODUCTION:Plant species within the prairie ecological zone of Canada are a source of natural products with important bio-activities. Investigation of these plants and the secondary metabolites that they produce will provide insight into their biology, and identify sources of natural products that may become new medicines or scientific tools. METHODS:We investigated a G1 phase arrest activity in extracts from the prairie plant, Thermopsis rhombifolia (Buffalo bean) by biology-guided fractionation and isolated luteolin. Cell based assays and CETSA were used to identify a cyclin-dependent kinase 9 inhibitory activity. RESULTS AND DISCUSSION: Luteolin treated cells showed decreased phosphorylation of the carboxy terminal domain of RNA polymerase II, and low levels of Mcl-1. Plant extracts or luteolin inhibited Cdk9 (cyclin dependent kinase) in tests in vitro, stabilized Cdk9 as determined by the cellular thermal shift assay (CETSA), and arrested cells in the G1 phase of the cell cycle. CONCLUSIONS:Luteolin joins an increasing number of flavonoid inhibitors that make convenient cell biology tools and contribute to our understanding of natural product biology in plants.

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Natural product extracts of the Canadian prairie plant,Thermopsis rhombifolia,have anti-cancer activity in phenotypic cell-based assays

Cited by 9 publications

References 16 publications

Genetic Resources of Crop Wild Relatives: A Canadian Perspective

Genetic Resources of Crop Wild Relatives: A Canadian Perspective

Extracts Prepared from a Canadian Toxic Plant Induce Light-Dependent Perinuclear Vacuoles in Human Cells

The Canadian Prairie Plant Thermopsis rhombifolia Contains Luteolin, a Flavone that Inhibits Cyclin Dependent Kinase 9 and Arrest Cells in the G1-Phase of the Cell Cycle

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