Natural Products Drug Discovery: On Silica or In-Silico?

Kim, Hye Kyong; Choi, Young Hae; Verpoorte, Robert

doi:10.1007/164_2022_611

Cited by 2 publications

(1 citation statement)

References 63 publications

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“…Hyphenated HPTLC and metabolomics are both excellent tools for simultaneous non-target bioprofiling of samples, whereby the investigation of the bioactivity is performed on-surface via HPTLC (Schreiner & Morlock, 2021 ; Schreiner et al, 2021 ) versus usually in silico via metabolomics. Both overcome laborious steps of bioactivity-guided fractionation of crude plant extracts (Kim et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Chemical and cytotoxicity profiles of 11 pink pepper (Schinus spp.) samples via non-targeted hyphenated high-performance thin-layer chromatography

Mügge

Morlock

2023

Metabolomics

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Introduction Pink pepper is a worldwide used spice that corresponds to the berries of two species, Schinus terebinthifolia Raddi or S. molle L. (Anacardiaceae). Toxic and allergic reactions by ingestion or contact with these plants were reported, and classical in vitro studies have highlighted the cytotoxic properties of apolar extracts from the fruits. Objectives Perform a non-targeted screening of 11 pink pepper samples for the detection and identification of individual cytotoxic substances. Methods After reversed-phase high-performance thin-layer chromatography (RP-HPTLC) separation of the extracts and multi-imaging (UV/Vis/FLD), cytotoxic compounds were detected by bioluminescence reduction from luciferase reporter cells (HEK 293 T-CMV-ELuc) applied directly on the adsorbent surface, followed by elution of detected cytotoxic substance into atmospheric-pressure chemical ionization high-resolution mass spectrometry (APCI-HRMS). Results Separations for mid-polar and non-polar fruit extracts demonstrated the selectivity of the method to different substance classes. One cytotoxic substance zone was tentatively assigned as moronic acid, a pentacyclic triterpenoid acid. Conclusion The developed non-targeted hyphenated RP-HPTLC–UV/Vis/FLD–bioluminescent cytotoxicity bioassay–FIA–APCI-HRMS method was successfully demonstrated for cytotoxicity screening (bioprofiling) and respective cytotoxin assignment.

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Section: Introductionmentioning

confidence: 99%