Natural variation of TaGASR7-A1 affects grain length in common wheat under multiple cultivation conditions

Dong, Lingli; Wang, Fang‐Ming; Liu, Tao; Dong, Zhenying; Li, Aili; Jing, Ruilian; Mao, Long; Li, Yiwen; Liu, Xin; Zhang, Kunpu; Wang, Daowen

doi:10.1007/s11032-014-0087-2

Cited by 86 publications

(52 citation statements)

References 41 publications

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“…With the high‐density genetic maps and reference genome sequences available for multiple crops, this strategy of using comparative genomics for understanding the genetic basis of trait control in related crops appeared to be effective and allowed to reduce time and efforts needed to identify causal genes. During the last several years, several genes controlling grain size and weight identified in rice were shown to underlie similar traits in wheat (Zhang et al ., ; Dong et al ., ; Wang et al ., ; Zhang et al ., ). Here, we characterized the wheat TaGW7 gene, the ortholog of the OsGW7 gene in rice.…”

Section: Discussionmentioning

confidence: 99%

Gene editing of the wheat homologs of TONNEAU1‐recruiting motif encoding gene affects grain shape and weight in wheat

et al. 2019

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Grain size and weight are important components of a suite of yield-related traits in crops. Here, we showed that the CRISPR-Cas9 gene editing of TaGW7, a homolog of rice OsGW7 encoding a TONNEAU1-recruiting motif (TRM) protein, affects grain shape and weight in allohexaploid wheat. By editing the TaGW7 homoeologs in the B and D genomes, we showed that mutations in either of the two or both genomes increased the grain width and weight but reduced the grain length. The effect sizes of mutations in the TaGW7 gene homoeologs coincided with the relative levels of their expression in the B and D genomes. The effects of gene editing on grain morphology and weight traits were dosage dependent with the double-copy mutant showing larger effect than the respective single copy mutants. The TaGW7-centered gene co-expression network indicated that this gene is involved in the pathways regulating cell division and organ growth, also confirmed by the cellular co-localization of TaGW7 with aand b-tubulin proteins, the building blocks of microtubule arrays. The analyses of exome capture data in tetraploid domesticated and wild emmer, and hexaploid wheat revealed the loss of diversity around TaGW7-associated with domestication selection, suggesting that TaGW7 is likely to play an important role in the evolution of yield component traits in wheat. Our study showed how integrating CRISPR-Cas9 system with cross-species comparison can help to uncover the function of a gene fixed in wheat for allelic variants targeted by domestication selection and select targets for engineering new gene variants for crop improvement.

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Section: Discussionmentioning

confidence: 99%

Gene editing of the wheat homologs of TONNEAU1‐recruiting motif encoding gene affects grain shape and weight in wheat

et al. 2019

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“…GASR7 genes are mostly hormone-regulated and encode proteins of the Snakin/ GASA family involved in signaling pathways that modulate hormonal responses [55]. TuGASR7 is associated with increased grain length and weight in the parental wheat species Triticum urartu [56] and TaGASR7 is a major genetic determinant of grain length in hexaploid wheat [57,58].…”

Section: Grain Sizementioning

confidence: 99%

Roadmap for Accelerated Domestication of an Emerging Perennial Grain Crop

DeHaan

Larson

López‐Marqués

et al. 2020

Trends in Plant Science

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“…TaGW2 , encoding an E3 ubiquitin ligase and associated with GW and TGW, was cloned based on the homologous rice gene GW2 (Su et al, 2011). Similarly, TaCwi-A1 associated with TGW (Ma et al, 2012; Jiang et al, 2015), TaGASR7-A1 for GL and yield (Dong et al, 2014), TaGS-D1 for GL and TGW (Zhang et al, 2014), and TaTGW6 encoding an indole-3-acetic acid (IAA)-glucose hydrolase (Hu et al, 2016) have been cloned based on rice gene sequences.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA

Zhang

Liu

et al. 2016

Front. Plant Sci.

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Thousand-grain weight (TGW) of wheat (Triticum aestivum L.) contributes significantly to grain yield. In the present study, a candidate gene associated with TGW was identified through specific-locus amplified fragment sequencing (SLAF-seq) of DNA bulks of recombinant inbred lines (RIL) derived from the cross between Jing 411 and Hongmangchun 21. The gene was located on chromosome 7A, designated as TaTGW-7A with a complete genome sequence and an open reading frame (ORF). A single nucleotide polymorphism (SNP) was present in the first exon between two alleles at TaTGW-7A locus, resulting in a Val to Ala substitution, corresponding to a change from higher to lower TGW. Cleaved amplified polymorphic sequence (CAPS) (TGW7A) and InDel (TG9) markers were developed to discriminate the two alleles TaTGW-7Aa and TaTGW-7Ab for higher and lower TGW, respectively. A major QTL co-segregating with TaTGW-7A explained 21.7–27.1% of phenotypic variance for TGW in the RIL population across five environments. The association of TaTGW-7A with TGW was further validated in a natural population and Chinese mini-core collections. Quantitative real-time PCR revealed higher transcript levels of TaTGW-7Aa than those of TaTGW-7Ab during grain development. High frequencies of the superior allele TaTGW-7Aa for higher TGW in Chinese mini-core collections (65.0%) and 501 wheat varieties (86.0%) indicated a strong and positive selection of this allele in wheat breeding. The molecular markers TGW7A and TG9 can be used for improvement of TGW in breeding programs.

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Natural variation of TaGASR7-A1 affects grain length in common wheat under multiple cultivation conditions

Cited by 86 publications

References 41 publications

Gene editing of the wheat homologs of TONNEAU1‐recruiting motif encoding gene affects grain shape and weight in wheat

Gene editing of the wheat homologs of TONNEAU1‐recruiting motif encoding gene affects grain shape and weight in wheat

Roadmap for Accelerated Domestication of an Emerging Perennial Grain Crop

Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA

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