Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maize

Gao, Huajian; Cui, Junjun; Liu, Shengxue; Wang, Shuhui; Lian, Yongyan; Bai, Yunting; Zhu, Tengfei; Wu, Haohao; Wang, Yijie; Yang, Shiping; Li, Xuefeng; Zhuang, Junhong; Chen, Limei; Gong, Zhizhong; Qin, Feng

doi:10.1016/j.molp.2022.08.009

Cited by 51 publications

(27 citation statements)

References 52 publications

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“…A recent study demonstrated that three non‐synonymous polymorphisms in the ZmSRO1d locus (a homolog of Arabidopsis SRO1 ) associated with drought tolerance in maize seedlings also promote stomatal closure (Gao et al, 2022). ZmSRO1d‐R, encoded by the drought‐resistant allele, localizes to the plasma membrane by interacting with ZmRBOHC, a homolog of Arabidopsis RBOHF.…”

Section: Advances In the Genetic Dissection Of Drought Resistance In ...mentioning

confidence: 99%

“…ZmSRO1d‐R promotes the adenosine diphosphate‐ribosylation of ZmRBOHC to increase ROS accumulation in guard cells, thus inducing stomatal closure. Knockout of ZmRBOHC reduced drought tolerance in maize seedlings (Gao et al, 2022).…”

Section: Advances In the Genetic Dissection Of Drought Resistance In ...mentioning

confidence: 99%

“…The drought‐resistant allele ZmSRO1d‐R increases ROS accumulation and promotes stomatal closure to enhance drought resistance but provides an approximately 8.6% yield penalty under well‐watered conditions (Gao et al, 2022). Similarly, the ZmSRO1d‐S genotype increases drought resistance by slightly reducing stomatal aperture, which does not influence yields under well‐watered conditions (Gao et al, 2022). These findings point to a mechanism for the trade‐off between drought resistance and yield and provide a moderate natural variant for improving drought resistance via breeding (Figure 5).…”

Section: Developing Techniques To Improve Drought Resistancementioning

confidence: 99%

“…The drought tolerance of crop seedlings can be quantified by calculating their SR after severe drought. Many GWASs of SR have been performed in maize and wheat (Triticum aestivum) seedlings after severe drought, leading to the identification of several natural variants associated with drought tolerance (Liu et al, 2013;Mao et al, 2015Mao et al, , 2020Mao et al, , 2022aWang et al, 2016c;Xiang et al, 2017;Zhang et al, 2020b;Gao et al, 2022;Mei et al, 2022;Sun et al, 2022b).…”

Section: Genes That Function In Drought Resistance During the Seedlin...mentioning

confidence: 99%

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The battle of crops against drought: Genetic dissection and improvement

Yang

Qin

2023

JIPB

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With ongoing global climate change, water scarcity‐induced drought stress remains a major threat to agricultural productivity. Plants undergo a series of physiological and morphological changes to cope with drought stress, including stomatal closure to reduce transpiration and changes in root architecture to optimize water uptake. Combined phenotypic and multi‐omics studies have recently identified a number of drought‐related genetic resources in different crop species. The functional dissection of these genes using molecular techniques has enriched our understanding of drought responses in crops and has provided genetic targets for enhancing resistance to drought. Here, we review recent advances in the cloning and functional analysis of drought resistance genes and the development of technologies to mitigate the threat of drought to crop production.

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Section: Advances In the Genetic Dissection Of Drought Resistance In ...mentioning

confidence: 99%

Section: Advances In the Genetic Dissection Of Drought Resistance In ...mentioning

confidence: 99%

Section: Developing Techniques To Improve Drought Resistancementioning

confidence: 99%

Section: Genes That Function In Drought Resistance During the Seedlin...mentioning

confidence: 99%

See 2 more Smart Citations

The battle of crops against drought: Genetic dissection and improvement

Yang

Qin

2023

JIPB

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“…By genome-wide association study, ZmSRO1d is identified as a critical gene regulating drought resistance. Overexpression of ZmSRO1d enhances drought resistance by activating the mono-ADP-ribosyltransferase activity of ZmRBOHC, which increases ROS levels in guard cells and promotes stomatal closure [ 65 ]. Two calcium-dependent proteins, ZmCPK35 and ZmCPK37, enhance maize drought tolerance by activating anion channel ZmSLAC1 in guard cells and promoting stomatal closure [ 66 ].…”

Section: Drought Resistance Improvement: From Arabidopsis To Cropsmentioning

confidence: 99%

Signaling Transduction of ABA, ROS, and Ca2+ in Plant Stomatal Closure in Response to Drought

Liu

Song

Zhang

et al. 2022

IJMS

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Drought is a global threat that affects agricultural production. Plants have evolved several adaptive strategies to cope with drought. Stomata are essential structures for plants to control water status and photosynthesis rate. Stomatal closure is an efficient way for plants to reduce water loss and improve survivability under drought conditions. The opening and closure of stomata depend on the turgor pressure in guard cells. Three key signaling molecules, including abscisic acid (ABA), reactive oxygen species (ROS), and calcium ion (Ca2+), play pivotal roles in controlling stomatal closure. Plants sense the water-deficit signal mainly via leaves and roots. On the one hand, ABA is actively synthesized in root and leaf vascular tissues and transported to guard cells. On the other hand, the roots sense the water-deficit signal and synthesize CLAVATA3/EMBRYO-SURROUNDING REGION RELATED 25 (CLE25) peptide, which is transported to the guard cells to promote ABA synthesis. ABA is perceived by pyrabactin resistance (PYR)/PYR1-like (PYL)/regulatory components of ABA receptor (RCAR) receptors, which inactivate PP2C, resulting in activating the protein kinases SnRK2s. Many proteins regulating stomatal closure are activated by SnRK2s via protein phosphorylation. ABA-activated SnRK2s promote apoplastic ROS production outside of guard cells and transportation into the guard cells. The apoplastic H2O2 can be directly sensed by a receptor kinase, HYDROGEN PEROXIDE-INDUCED CA2+ INCREASES1 (HPCA1), which induces activation of Ca2+ channels in the cytomembrane of guard cells, and triggers an increase in Ca2+ in the cytoplasm of guard cells, resulting in stomatal closure. In this review, we focused on discussing the signaling transduction of ABA, ROS, and Ca2+ in controlling stomatal closure in response to drought. Many critical genes are identified to have a function in stomatal closure under drought conditions. The identified genes in the process can serve as candidate genes for genetic engineering to improve drought resistance in crops. The review summarizes the recent advances and provides new insights into the signaling regulation of stomatal closure in response to water-deficit stress and new clues on the improvement of drought resistance in crops.

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Gene editing of ZmGA20ox3 improves plant architecture and drought tolerance in maize

Liu,

Chen,

Zhang

et al. 2023

Plant Cell Rep

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Drought stress, a major plant abiotic stress, is capable of suppressing crop yield performance severely.However, the trade-off between crop drought tolerance and yield performance turns out to be signi cantly challenging in drought-resistant crop breeding. Several phytohormones (e.g., gibberellin (GA)) have been reported to play a certain role in plant drought response, which also take on critical signi cance in plant growth and development. In this study, the loss-of-function mutations of GA biosynthesis enzyme ZmGA20ox3 were produced using the CRISPR-Cas9 system in maize. As indicated by the result of twoyear eld trials, the above-mentioned mutants displayed semi-dwar ng phenotype with the decrease of GA 1 , and almost no yield loss was generated compared with wild-type (WT) plants. Interestingly, as revealed by the transcriptome analysis, differential expressed genes (DEGs) were notably enriched in abiotic stress progresses, and biochemical tests indicated the signi cantly increased ABA, JA, and DIMBOA levels in mutants, suggesting that ZmGA20ox3 may take on vital signi cance in stress response in maize. The in-depth analysis suggested that the loss function of ZmGA20ox3 can enhance drought tolerance in maize seedling, reduce Anthesis-Silking Interval (ASI) delay while decreasing the yield loss signi cantly in the eld under drought conditions. The results of this study supported that regulating ZmGA20ox3 can improve plant height while enhancing drought resistance in maize, thus serving as a novel method for drought-resistant genetic improvement in maize. Key MessageEditing ZmGA20ox3 can achieve the effect similar to applying Cycocel, which can reduce maize plant height and enhance stress resistance.

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Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maize

Cited by 51 publications

References 52 publications

The battle of crops against drought: Genetic dissection and improvement

The battle of crops against drought: Genetic dissection and improvement

Signaling Transduction of ABA, ROS, and Ca2+ in Plant Stomatal Closure in Response to Drought

Gene editing of ZmGA20ox3 improves plant architecture and drought tolerance in maize

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