NATURALLY-OCCURRING HEMAGGLUTININ IN A TUNICATE <i>HALOCYNTHIA PYRIFORMIS</i>

Anderson, Robert S.; Good, Robert A.

doi:10.2307/1540513

Cited by 32 publications

(8 citation statements)

References 29 publications

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“…The presence of haemagglutinins in tunicate haemolymph has also been reported (Fuke and Sugai, 1972;Bretting and Renwrantz, 1973;Wright, 1974;Anderson and Good, 1975;Parrinello and Patricolo, 1975), and it has been suggested that the phagocytosis of foreign erythrocytes by haemocytes involves the participation of these molecules, i.e. they may function as opsonins.…”

Section: Introductionmentioning

confidence: 72%

“…It has been known for some time that inert particles can be phagocytosed by cells circulating in the haemolymph of tunicates (Fulton, 1920;Anderson, 1971;Brown and Davies, 1971;Bang, 1975). The presence of haemagglutinins in tunicate haemolymph has also been reported (Fuke and Sugai, 1972;Bretting and Renwrantz, 1973;Wright, 1974;Anderson and Good, 1975;Parrinello and Patricolo, 1975), and it has been suggested that the phagocytosis of foreign erythrocytes by haemocytes involves the participation of these molecules, i.e. they may function as opsonins.…”

Section: Introductionmentioning

confidence: 97%

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HAEMAGGLUTININ LEVELS IN HAEMOLYMPH FROM THE COLONIAL ASCIDIAN BOTRYLLOIDES LEACHII FOLLOWING INJECTION WITH SHEEP OR CHICKEN ERYTHROCYTES

Coombe

Jenkin

1982

Aust J Exp Biol Med

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Summary.Haemolymph was collected from colonies ot Botryltoides leachii killed at various times after injection with sheep or chicken erythrocytes and the agglutination titre for sheep, chicken and guinea-pig erythrocytes was determined. Controls included haemolymph from groups of uninjected colonies and from others injected with balanced salt solution. Animals given a single injection of erythrocytes exhibited no change in titre until the second week when a marginal (2-to 4-fold) but statistically significant rise in titre was detected using each of the 3 types of indicator erythrocytes. A return to control values was observed by 6 weeks. The response to a second injection of erythrocytes, given 6 weeks after the first, appeared to be identical in magnitude and time of onset to the primary response, i.e. there was no indication of an anamnestic response. The increased haemagglutinating activity apparent 2 weeks after each injection was attributable to a rise in the HA-2 agglutinin specifically, since the activity of the HA-1 agglutinin (a calcium-dependent lectin which binds to guinea-pig erythrocytes only) remained unchanged. The results are considered in relation to self: non-self recognition in this species.

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Section: Introductionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 97%

HAEMAGGLUTININ LEVELS IN HAEMOLYMPH FROM THE COLONIAL ASCIDIAN BOTRYLLOIDES LEACHII FOLLOWING INJECTION WITH SHEEP OR CHICKEN ERYTHROCYTES

Coombe

Jenkin

1982

Aust J Exp Biol Med

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“…; Gold et al . ; Roche & Monsigny ; Ryoyama ; Anderson & Good ). The HA activity of serum agglutinin of P. lepida against rabbit RBC could be inhibited by monosaccharide galactose at the MIC of 12.5 mM.…”

Section: Discussionmentioning

confidence: 99%

“…It is known that the specificity of most cell membrane receptors against various hemagglutinins largely depends on the sugar present in the receptor molecule. Simple sugars are capable of neutralizing hemagglutinins, thereby suggesting their specificity to the sugar moiety (Mäkelä 1957;Johnson 1964;McDade & Tripp 1967;Bird et al 1972;Gold et al 1974;Roche & Monsigny 1974;Ryoyama 1974;Anderson & Good 1975). The HA activity of serum agglutinin of P. lepida against rabbit RBC could be inhibited by monosaccharide galactose at the MIC of 12.5 mM.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of natural lectin with bacterial agglutination property in the serum of lepidopteran pest, Parasa lepida

Arumugam

Hilda

Bhuvaragavan

et al. 2019

Entomological Science

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Insects depend on lectins for non‐self recognition and clearance of invading pathogens. Naturally occurring lectin showing specificity for galactose was purified from the serum of lepidopteran pest Parasa lepida by affinity chromatography using Sepharose 6B coupled with galactose as a gel matrix. Preliminary studies on crude serum agglutinin revealed that the agglutinin molecule showed varying degrees of specificity to avian and mammalian red blood cells tested. Among them, the highest titer of 128 was recorded against rabbit red blood cell type. The agglutinin molecule in the crude serum was stable up to 60°C and at pH between 6 and 9. Also, the hemagglutinating activity was neither dependent on divalent cations nor sensitive to ethylenediaminetetraacetic acid treatment. Galactose inhibited the hemagglutinating activity at minimum inhibitory concentration of 12.5 mM and hence it was used as a ligand for affinity chromatography. Native polyacrylamide gel electrophoresis analysis revealed a single band and the molecular weight of the lectin was found to be approximately 90 kDa. Bacterial agglutination activity of the purified lectin with two significant toxin bacteria, namely Salmonella typhi and Bacillus thuringiensis, was observed.

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“…This method will be applicable to the study of the agglutinin receptors of intact cells, such as murine tumor cells.We examined the effects of Ca2+ on the binding of FITC-BRAs. Ca2+ is required for the binding of naturally-occurring invertebrate agglutinins, such as those from Limulus, lobster, oyster, and a tunicate 26…”

mentioning

confidence: 99%

Comparison of the multiple agglutinins of the acorn barnacle, Megabalanus rosa.

Muramoto

Ogata

Kamiya

1985

Agricultural and Biological Chemistry

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We investigated the subunit structures of the three agglutinins (BRA-1, BRA-2, and BRA-3) isolated from the coelomic fluid of the acorn barnacle, Megabalanus rosa, by high-speed gel filtration, gel electrophoresis, CD spectra, and peptide mapping. BRA-2 (Mr 140,000) and BRA-3 (Mr 64,000) were glycoproteins composed of subunits having two identical basic units cross-linked by disulfide bonds. The basic units of BRA-2 and BRA-3 had molecular weights of 22,000 and 18,000, respectively. They did not have a common structure. BRA-1 (Mr 330,000) was composed of subunits homologous to that of BRA-2. The binding properties of these agglutinins against rabbit erythrocyte ghosts were also investigated after labeling the agglutinins with fluorescein isothiocyanate.

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NATURALLY-OCCURRING HEMAGGLUTININ IN A TUNICATE HALOCYNTHIA PYRIFORMIS

Cited by 32 publications

References 29 publications

HAEMAGGLUTININ LEVELS IN HAEMOLYMPH FROM THE COLONIAL ASCIDIAN BOTRYLLOIDES LEACHII FOLLOWING INJECTION WITH SHEEP OR CHICKEN ERYTHROCYTES

HAEMAGGLUTININ LEVELS IN HAEMOLYMPH FROM THE COLONIAL ASCIDIAN BOTRYLLOIDES LEACHII FOLLOWING INJECTION WITH SHEEP OR CHICKEN ERYTHROCYTES

Occurrence of natural lectin with bacterial agglutination property in the serum of lepidopteran pest, Parasa lepida

Comparison of the multiple agglutinins of the acorn barnacle, Megabalanus rosa.

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