Nature and distribution of feline sarcoma virus nucleotide sequences

Frankel, Arthur E.; Gilbert, James H.; Porzig, Klaus J.; Scolnick, Edward M.; Aaronson, Stuart A.

doi:10.1128/jvi.30.3.821-827.1979

Cited by 127 publications

(42 citation statements)

References 32 publications

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“…The hybridization of cDNAs with salmon DNA was close to background level, and that with E. coli was considered negative. These results demonstrate that, like unique sequences of RSV, acute leukemia viruses, and other RNA tumor viruses (15,16,20,42,48,50), cellular sequences related tofps, yes, and ros are present in a wide variety of vertebrates, and the degree of homology correlates with the phylogenetic relationship.…”

Section: Resultsmentioning

confidence: 73%

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Cellular Sequences Related to Three New onc Genes of Avian Sarcoma Virus ( fps, yes , and ros ) and Their Expression in Normal and Transformed Cells

Shibuya

Hanafusa

Balduzzi

1982

J Virol

125

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Two onc genes of avian sarcoma viruses unrelated to the src gene have recently been identified: fps of Fujinami sarcoma virus/PRCII/UR1 and yes of Y73/Esh sarcoma virus. In the first part of this study we demonstrated that UR2, the most recently isolated avian sarcoma virus, contains in its genome a unique sequence, ros, nonhomologous to src, fps, and yes sequences or to transforming genes of avian acute leukemia viruses. Using cDNAs specific to the inserts of avian sarcoma virus genomes, we examined the existence and the transcription of cellular nucleotide sequences related to the three new onc genes of avian sarcoma virus (fps, yes and ros) in various cells. The progenitor cellular sequences for these onc genes (c-onc) were present in uninfected chicken DNA in one or few copies per haploid genome. These c-onc sequences were detectable in cellular DNA of a wide variety of vertebrates, and the homology between viral and cellular onc was inversely related to the phylogenetic distance of animal species.

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Section: Resultsmentioning

confidence: 73%

“…a Total cellular RNA was extracted from uninfected CEF or quail embryo fibroblasts (QEF) and from ASV-transformed CEF. 3H-labeled cDNA0nc probes (500 cpm) were incubated with various amounts (0.06 to 100 ,ug) of each RNA in 8 to 16 ,ul for 100 h under the same conditions described in the legend to Fig. 1.…”

Section: Resultsmentioning

confidence: 99%

Cellular Sequences Related to Three New onc Genes of Avian Sarcoma Virus ( fps, yes , and ros ) and Their Expression in Normal and Transformed Cells

Shibuya

Hanafusa

Balduzzi

1982

J Virol

125

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“…For preparation of feeder layers or conditioned medium, NFS/N bone marrow cell suspen- 6 sions (10 nucleated cells/ml) were plated in 60-mm tissue culture plates in RPMI 1640 medium supplemented with 20% horse serum (Flow Laboratories, Rockville, MD) and 5 × 10 -5 ME. Nonadherent cells and medium were removed after 5 d, and medium containing 15% fetal calf serum was used thereafter.…”

Section: Establishment Of Balb-msv-and Harvey-msv-transformed Hematopmentioning

confidence: 99%

BALB- and Harvey-murine sarcoma virus transformation of a novel lymphoid progenitor cell.

Pierce¹,

Aaronson²

1982

The Journal of Experimental Medicine

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BALB- and Harvey-murine sarcoma viruses (MSV) comprise a family of retroviruses whose mouse- and rat-derived onc genes are closely related. These viruses induce sarcomas and erythroleukemias in susceptible animals. An in vitro colony assay that detects transformation of lymphoid cells by Abelson-murine leukemia virus was used to demonstrate that BALB- and Harvey-MSV transform a novel hematopoietic cell both in culture and in vivo. Bone marrow colony formation was sarcoma virus dependent, followed single-hit kinetics, and required the presence of mercaptoethanol in the agar medium. BALB- and Harvey-MSV-induced colonies could be established in culture as continuous cell lines that demonstrated unrestricted self-renewal capacity and leukemogenicity in vivo. The cells had a blast cell morphology and lacked detectable markers of mature cells within the myeloid or erythroid series. They also lacked detectable immunoglobulin mu chain or Thy-1 antigen, markers normally associated with committed cells of the B and T lymphoid lineages, respectively. However, the transformants contained very high levels of terminal deoxynucleotidyl transferase (TdT), an enzyme believed to be specific to early stages within the lymphoid differentiation pathway. This phenotype distinguishes these BALB- and Harvey-MSV transformants from any previously reported hematopoietic targets of transforming retroviruses, including the pre-B lymphoid cell transformed by Abelson-MuLV under identical assay conditions. These newly identified lymphoid progenitor cell transformants may provide an important means of studying early stages of lymphoid ontogeny and the possible role of TdT in lymphoid development.

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“…We suspect that the fate of transfected retroviral DNA molecules differs from the fate of viral DNA molecules synthesized within a cell after retrovirus infection. Other investigators have shown that nonproducer transformed cells derived by sarcoma virus infection can have as few as one copy of proviral DNA per haploid genome (8).…”

Section: Resultsmentioning

confidence: 99%

Structure of the provirus within NIH 3T3 cells transfected with Harvey sarcoma virus DNA

Goldfarb¹,

Weinberg²

1981

J Virol

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NIH 3T3 cells transformed with unintegrated Harvey sarcoma virus (HSV) linear DNA generally acquired a complete HSV provirus. Infection of these transformed cells with Moloney murine leukemia helper virus was followed by release of infectious particles. The HSV provirus within these transfected cells was convalently joined to nonviral DNA sequences and was terned "cell-linked" HSV DNA. The association of this cell-virus DNA sequence with the chromosomal DNA of a transfected cell was unclear. NIH 3T3 cells could also become transformed by transfection with this cell-linked HSV DNA. In this case, the recipient cells generally acquired a donor DNA fragment containing both the HSV provirus and its flanking nonviral sequences. After celLs acquired either unintegrated or cell-linked HSV DNA, the newly established provirus and flanking cellular sequences underwent amplification to between 5 and 100 copies per diploid cell. NIH 3T3 cells transfected with HSV DNA may acquire deleted proviral DNA lacking at least 1.3 kilobase pairs from the right end of full-length HSV 6-kilobase-pair DNA (corresponding to the 3'-proximal portion of wild-type HSV RNA). Cells bearing such deleted HSV genomes were transfonned, indicating that the viral transformation gene lies in the middle or 5'-proximal portion of the HSV RNA genome. However, when these cells were infected with Moloney murine leukemia helper virus, only low levels of biologically active sarcoma virus particles were released. Therefore, the 3' end of full-length HSV RNA was required for efficient transmission of the viral genome.Harvey sarcoma virus (HSV) is a retrovirus that induces fibrosarcomas in infected rats and mice and causes morphological transformation of fibroblasts cultured in vitro. HSV originated through genetic recombination between a nonsarcomagenic retrovirus, Moloney murine leukemia virus (M-MLV), and rat cellular sequences (16,24). As Fig. 1 shows, HSV genomic RNA (size, 5.4 kilobases) contains a large internal rat cellular sequence, whereas the RNA 5' and 3' ends are derived from the 5' and 3' ends of genomic M-MLV RNA (5).The HSV genome replicates within an infected cell through DNA intermediates analogous to the DNA intermediates of other retroviruses. Shortly after infection, HSV RNA is reverse transcribed to generate a linear doublestranded DNA which is slightly longer (6.0 kilobase pairs [kbp]) than genomic RNA (11,20). As Fig. 1 shows, the 6-kbp DNA bears a terminal sequence repetition; nucleotide sequences det Present address: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724.on July 6, 2020 by guest

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Nature and distribution of feline sarcoma virus nucleotide sequences

Cited by 127 publications

References 32 publications

Cellular Sequences Related to Three New onc Genes of Avian Sarcoma Virus ( fps, yes , and ros ) and Their Expression in Normal and Transformed Cells

Cellular Sequences Related to Three New onc Genes of Avian Sarcoma Virus ( fps, yes , and ros ) and Their Expression in Normal and Transformed Cells

BALB- and Harvey-murine sarcoma virus transformation of a novel lymphoid progenitor cell.

Structure of the provirus within NIH 3T3 cells transfected with Harvey sarcoma virus DNA

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