Nature-inspired extracellular matrix coating produced by micro-patterned smooth muscle and endothelial cells endows cardiovascular materials with better biocompatibility

Han, Congzhen; Luo, Xiao; Zou, Dan; Li, Jingan; Zhang, Kun; Yang, Ping; Huang, Nan

doi:10.1039/c9bm00128j

Cited by 43 publications

(35 citation statements)

References 48 publications

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“…The surface biochemistry and biophysical characteristics of biomaterial scaffold are the key factors of cell adhesion, growth, proliferation and differentiation [ 3 , 17 , 23 ]. For example, ECM stiffness can regulate the integrity, permeability and leukocyte migration of vascular EC monolayers [ 12 , 23 , 25 , 38 , 39 ]. Moreover, controlling nanotopography and mechanical properties can be used to stimulate cells by mimicking their natural environment, triggering the correct functionalities [ 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

“…Studies have shown that hyaluronic acid (HA) has double functionality as anticoagulant and promoter of endothelialization [ 21 , 22 , 23 ]. HA, as a unique glycosaminoglycan, widely exists in the ECM of various tissues [ 10 , 24 , 25 ]. It has been recognized that HA plays a key role in adjusting biological functions by binding to specific protein receptors (such as CD44) on different cell surfaces [ 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

Niu

2021

Nanomaterials

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In this study, we designed and synthetized artificial vascular scaffolds based on nanofibers of collagen functionalized with hyaluronic acid (HA) in order to direct the phenotypic shape, proliferation, and complete endothelization of mouse primary aortic endothelial cells (PAECs). Layered tubular HA/collagen nanofibers were prepared using electrospinning and crosslinking process. The obtained scaffold is composed of a thin inner layer and a thick outer layer that structurally mimic the layer the intima and media layers of the native blood vessels, respectively. Compared with the pure tubular collagen nanofibers, the surface of HA functionalized collagen nanofibers has higher anisotropic wettability and mechanical flexibility. HA/collagen nanofibers can significantly promote the elongation, proliferation and phenotypic shape expression of PAECs. In vitro co-culture of mouse PAECs and their corresponding smooth muscle cells (SMCs) showed that the luminal endothelialization governs the biophysical integrity of the newly formed extracellular matrix (e.g., collagen and elastin fibers) and structural remodeling of SMCs. Furthermore, in vitro hemocompatibility assays indicated that HA/collagen nanofibers have no detectable degree of hemolysis and coagulation, suggesting their promise as engineered vascular implants.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

Niu

2021

Nanomaterials

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“…ECs were inoculated into 24-well plates at a density of 1.5 × 10 3 cells and cultured in the same way as above. Finally, the cells stained by AO/EB double-staining kit (Solabio) were observed by laser confocal microscopy (Nikon C2 Plus, Tokyo, Japan) 32…”

Section: Methodsmentioning

confidence: 99%

“…Finally, the cells stained by AO/EB double-staining kit (Solabio) were observed by laser confocal microscopy (Nikon C2 Plus, Tokyo, Japan). 32…”

Section: Ao/eb Staining Of Ecsmentioning

confidence: 99%

Effects of degradation products of biomedical magnesium alloys on nitric oxide release from vascular endothelial cells

et al. 2019

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Nitric oxide (NO) released by vascular endothelial cells (VECs), as a functional factor and signal pathway molecule, plays an important role in regulating vasodilation, inhibiting thrombosis, proliferation and inflammation. Therefore, numerous researches have reported the relationship between the NO level in VECs and the cardiovascular biomaterials’ structure/functions. In recent years, biomedical magnesium (Mg) alloys have been widely studied and rapidly developed in the cardiovascular stent field for their biodegradable absorption property. However, influence of the Mg alloys’ degradation products on VEC NO release is still unclear. In this work, Mg-Zn-Y-Nd, an Mg alloy widely applied on the biodegradable stent research, was investigated on the influence of the degradation time, the concentration and reaction time of degradation products on VEC NO release. The data showed that the degradation product concentration and the reaction time of degradation products had positive correlation with NO release, and the degradation time had negative correlation with NO release. All these influencing factors were controlled by the Mg alloy degradation behaviors. It was anticipated that it might make sense for the cardiovascular Mg alloy design aiming at VEC NO release and therapy.

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“…Human umbilical vein endothelial cells (HUVEC, purchased from Chengdu Hao Yi Biotechnology Co., Ltd., Chengdu, China) ranged from the third to the fifth passages were seeded on the 6-well plate with concentration of 7 × 10 3 cells/well, and cultured with the 1640 medium containing MgNPs, MgNPb, HA/PEIs (small size), HA/ PEIb (big size) and the pure MgCl 2 at 37°C, with 5% CO 2 [29]. After incubated for 1, 6, 12 h, 1 day, 3 days and 5 days, the HUVEC were cleaned with phosphate buffer solution (PBS, pH 7.4) and fixed with 4% paraformaldehyde [30]. The fixed HUVEC were fluorescence-stained with CD31 antibody (Sigma, USA) and 4, 6-diamino-2-phenyl indole (DAPI, Sigma, USA), and recorded by a fluorescence microscope (DMRX, Leica, Germany) [19].…”

Section: Endothelial Functional Testsmentioning

confidence: 99%

Preparing a novel magnesium‐doped hyaluronan/polyethyleneimine nanoparticle to improve endothelial functionalisation

Wang

Zhu

Wang

et al. 2020

IET nanobiotechnol.

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Nowadays, tissue engineering vascularisation has become an important means of organ repair and treatment of major traumatic diseases. Vascular endothelial layer regeneration and endothelial functionalisation are prerequisites and important components of tissue engineering vascularisation. The present researches of endothelial functionalisation mainly focus on tissue engineering scaffold preparation and implant surface modification. Few studies have reported the interaction of endothelial functionalisation and scaled materials, especially the nanomaterials. Magnesium (Mg), as an essential cytotropic active element in the human body, should promote the growth of endothelial cells. However, the authors' previous work found that the Mg in the alloys had a defect of delayed endothelialisation, which may be attributed to the non-uniform scales of the degradation products from Mg alloys. To validate this hypothesis and fabricate a novel nanomaterial for tissue engineering vascularisation, the authors prepared Mg-doped hyaluronan (HA)/polyethyleneimine (PEI) nanoparticles for endothelial cells testing. Their data showed that the Mg-doped HA/PEI nanoparticle with small scales (diameter <150 nm) presented better ability on improving endothelial cells growth, functionalisation and nitric oxide release.

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Nature-inspired extracellular matrix coating produced by micro-patterned smooth muscle and endothelial cells endows cardiovascular materials with better biocompatibility

Abstract: Functionalizing cardiovascular biomaterials with an extracellular matrix (ECM) via in vitro decellularization has been applied as an effective method to improve the biocompatibility of implants.

Cited by 43 publications

References 48 publications

Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

Hyaluronic Acid/Collagen Nanofiber Tubular Scaffolds Support Endothelial Cell Proliferation, Phenotypic Shape and Endothelialization

Effects of degradation products of biomedical magnesium alloys on nitric oxide release from vascular endothelial cells

Preparing a novel magnesium‐doped hyaluronan/polyethyleneimine nanoparticle to improve endothelial functionalisation

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