Effects of degradation products of biomedical magnesium alloys on nitric oxide release from vascular endothelial cells

Abstract

Nitric oxide (NO) released by vascular endothelial cells (VECs), as a functional factor and signal pathway molecule, plays an important role in regulating vasodilation, inhibiting thrombosis, proliferation and inflammation. Therefore, numerous researches have reported the relationship between the NO level in VECs and the cardiovascular biomaterials’ structure/functions. In recent years, biomedical magnesium (Mg) alloys have been widely studied and rapidly developed in the cardiovascular stent field for their biodegradable absorption property. However, influence of the Mg alloys’ degradation products on VEC NO release is still unclear. In this work, Mg-Zn-Y-Nd, an Mg alloy widely applied on the biodegradable stent research, was investigated on the influence of the degradation time, the concentration and reaction time of degradation products on VEC NO release. The data showed that the degradation product concentration and the reaction time of degradation products had positive correlation with NO release, and the degradation time had negative correlation with NO release. All these influencing factors were controlled by the Mg alloy degradation behaviors. It was anticipated that it might make sense for the cardiovascular Mg alloy design aiming at VEC NO release and therapy.

The application of tissue-engineered fish swim bladder vascular graft

“…To show that rapamycin was bonded to the surface of the decellularized swim bladder, we measured the water contact angles (WCA) of the decellularized swim bladder before and after rapamycin bonding, the WCA was measured on the hyaluronic acid rapamycin-coated decellularized swim bladder; morphology was assessed with SEM 45 . Patches conjugated with nanoparticle rapamycin were incubated in PBS at 37 degrees, and the supernatant of each sample was collected and absorption (400 nm) was analyzed at each time point using a SpectraMax plate reader (Molecular Devices, Sunnyvale, CA).…”

Application of the Tissue-Engineered Plant Scaffold as a Vascular Patch

“…The procedures followed were as described previously. 28 After freeze-drying, the samples were fixed on a small bracket, gold sprayed, and observed under the SEM (Auriga, Zeiss, Germany).…”