Nature of the Genetic Blocks in the Isoleucine-Valine Mutants of Salmonella

Wagner, Robert P.; Bergquist, Arloa

doi:10.1093/genetics/45.10.1375

Cited by 26 publications

(6 citation statements)

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“…A small portion of these data are shown in Tables 2 and 3. Six ilua strains isolated since the reports by GLAN-VILLE and DEMEREC (1960) and WAGNER and BERGQUIST (1960) were tested by transduction procedures with known ilua strains (Table 2) and by assay of cellfree preparations for reductoisomerase and a, P-dihydroxy acid dehydrase activities (Table 3 ) . By both criteria the six strains classify as iluaB.…”

Section: Resultsmentioning

confidence: 99%

“…Strain iluaD-27 is capable of suboptimal growth on a valine supplement (GLANVILLE and DEMEREC 1960;WAGNER and BERGQUIST 1960), and recent growth studies provide evidence that iluaA-28 also exhibits this property. Strain iluaA-12 has been observed to utilize valine alone as a growth supplement, but this observation is not always reproducible with this particular strain.…”

Section: Resultsmentioning

confidence: 99%

“…Auxanography was carried out as described by WAGNER and BERGQUIST (1960), except that single-enriched minimal agar plates were used instead of minimal; the utilization of the d hdroxy acid precursors of isoleucine and valine is more pronounced on the single-enriched medium.…”

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confidence: 99%

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Isoleucine-Valine Requiring Mutants of Salmonella Typhimurium

Armstrong

Wagner

1964

Genetics

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initial reports on the mutant strains of Salmonella typhimurium that T : e : u i r e both isoleucine and valine for growth (ilva strains) were published concomitantly by GLANVILLE and DEMEREC BERGQUIST (1960). These studies showed that although the available ilva strains could be divided into four complementation groups ( A , B, C and D ) on the basis of abortive transduction results, only three loci, as determined by enzymatic analysis, were involved. These results are illustrated in Figure 1. Both the iZvaA strains and the single ilvaD strain are deficient in reductoisomerase. Various attempts to establish definite biochemical differences between these two groups have not been successful ( ARMSTRONG and WAGNER 1962) , and it is currently thought that the iZvaA and ilvaD designations may represent complementing subunits within the locus that controls the production of the reductoisomerase. A detailed discusson of complementation within a locus is found in the report by AMES and HARTMAN (1962) on the genetics of histidine biosynthesis in S. typhimurium.The transduction studies further showed that the tranducing fragment bearing the ilva loci also contains the locus (&A) that controls the production of a-ketobutyrate, a precursor of isoleucine (Figure 1 ) . ' This irireitigation was supported by Research Grants GM 10166-02 and RG-6492 ( C 3 ) and Training Grant 2G-337

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Ultraviolet Lightmentioning

confidence: 99%

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Isoleucine-Valine Requiring Mutants of Salmonella Typhimurium

Armstrong

Wagner

1964

Genetics

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“…We have not attempted enzymatic analysis of the mutants studied here; and therefore the precise position of the block in each group is not definite, except in the case of the isoleucine-valine mutants. These have been analyzed biochemically by DR. R. P. WAGNER, whose results are reported in the paper following this one (WAG-NER and BERGQUIST 1960).…”

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confidence: 87%