NC/Nga Mice: A Mouse Model for Atopic Dermatitis

Suto, Hajime; Matsuda, Hiroshi; Mitsuishi, Kouichi; Hira, Kayako; Uchida, Takafumi; Unno, Tetsushi; Ogawa, Hideoki; Ra, Chisei

doi:10.1159/000053599

Cited by 222 publications

(112 citation statements)

References 10 publications

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“…The total clinical score for the severity is defined as the sum of individual scores graded as 0 (subclinical), 1 (mild), 2 (moderate), 3 (severe), and 4 (very severe) as described previously. 14) Additionally, photographs of representative mice exhibiting average clinical symptoms in each group were taken after the majority of mice had shown AD-like skin lesions.…”

Section: Methodsmentioning

confidence: 99%

Anti-Allergic Effect of a Hot-Water Extract of Quince (Cydonia oblonga)

Shinomiya

Hamauzu

Kawahara

2009

Bioscience, Biotechnology, and Biochemistry

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We examined the effect of a crude hot-water extract (HW) of quince (Cydonia oblonga Miller) fruit on type I allergy in vivo and in vitro. The oral administration of the quince HW-added diet to NC/Nga mice for 63 d showed a significant decrease in the development of atopic dermatitis-like skin lesions under conventional conditions. The concentration of IgE in the serum collected from mice fed with quince HW was also lowered in a dose-dependent manner. Moreover, we found that quince HW inhibited the release of -hexosaminidase from rat basophilic leukemia cell line RBL-2H3 after a 24-h treatment. The quince HW fraction of less than 3 kDa reduced the mRNA expression of the high-affinity IgE receptor (Fc"RI) subunit. These results suggest that quince HW had an inhibitory effect on type I allergy by suppressing IgE production and IgE-mediated degranulation.

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Section: Methodsmentioning

confidence: 99%

Anti-Allergic Effect of a Hot-Water Extract of Quince (Cydonia oblonga)

Shinomiya

Hamauzu

Kawahara

2009

Bioscience, Biotechnology, and Biochemistry

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“…Our findings, however, indicate the strong probability that S. aureus does not initiate, but rather aggravates dermatitis in this strain. The NC/Nga strain is now thought to be a suitable model for certain aspects of human atopic dermatitis [3,6]. Since clinico-pathlogic features of dermatitis under conventional conditions are similar between DSNh and NC/Nga mice, there is some possibility that the DS-Nh strain is another mouse model of human atopic dermatitis.…”

mentioning

confidence: 99%

Spontaneous Development of Dermatitis in DS-Nh Mice under Specific Pathogen-Free Conditions.

et al. 2003

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Spontaneous development of dermatitis in DS-Nh mice under specific pathogenfree conditions was examined to verify the hypothesis [Exp. Anim. 46: 225-229, 1997] that Stapylococcus aureus (S. aureus) infection is causally associated with the dermatitis. Observation of the mice up to 28 weeks of age indicated that obvious dermatitis does occur under S. aureus-free conditions, though the incidence was low (six of 42 females and two of 90 males). Skin lesions in the absence of this bacterium showed histological changes very similar to those that can be observed under conventional conditions. In addition, hyperproduction of serum IgE was demonstrated in the dermatitis-positive mouse. These findings suggested that the dermatitis is triggered by IgE-mediated allergic reactions. Key words: dermatitis, DS-Nh mouse, Stapylococcus aureusThe DS-Nh (DS Non-hair) mouse [1] is a spontaneous hairless mutant of the DS mouse [7] that has been established as an inbred strain from a closed colony of dd mice at Aburahi Laboratories, Shionogi & Co., Ltd. According to the report by Haraguchi et al. [1], spontaneous development of chronic dermatitis under conventional conditions is characteristic of the DS-Nh strain regardless of age or gender. Although the pathogenic mechanism of dermatitis is unclear at present, the authors proposed a hypothesis that Stapylococcus aureus (S. aureus) infection is causally associated with dermatitis, based on their findings that S. aureus was isolated from the skin lesion and that experimental infection with the isolates promoted onset of dermatitis. They also described as further evidence that no dermatitis (Received 17 April 2002 / Accepted 18 September 2002 Address corresponding: T. Hirasawa, Aburahi Laboratories, Shionogi & Co., Ltd., Gotanda 1405, Koka, Shiga 520-3423, Japan was observed under specific pathogen-free (SPF) conditions. However, the test was performed in a vinyl isolator for a short time (until the mice were 16 weeks of age) using only five animals in their study [1]. In addition, we have observed that a few DS-Nh mice developed dermatitis after 50 weeks of age under SPF conditions (unpublished data). Since the presence or absence of the dermatitis under SPF conditions is a critical point for evaluating the role of S. aureus, careful re-examination using an adequate number of mice appeared to be necessary.The present study demonstrated that dermatitis develops in this strain even under S. aureus-free conditions, though at a much lower incidence than that under conventional conditions.

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“…For this study, keratinized skin was produced in NC/Nga mice, which have been used as a model for atopic dermatitis because they develop atopic dermatitis-like symptoms, including excessive keratinization with thickening and drying of skin, when maintained under conventional conditions. 29,30) As presented in Fig. 5, a clear difference was observed between normal and keratinized skin.…”

Section: Formulation Effects On Terbinafine Skin Permeationmentioning

confidence: 79%

DA 5505: A Novel Topical Formulation of Terbinafine That Enhances Skin Penetration and Retention

Thapa

Han

Park

et al. 2015

CHEMICAL & PHARMACEUTICAL BULLETIN

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Topical fungal infections can become severe if left untreated. Efficient treatment modalities for topical fungal infections aid the penetration of antifungal agents deep into viable skin layers. Terbinafine is a fungicidal agent that inhibits ergosterol, an essential fungal component. The main objective of this study was to evaluate skin permeation and retention of a terbinafine-loaded solution containing chitosan as a film former. Comparative assessment of skin permeation and retention was performed using a prepared formulation (DA 5505) and marketed formulations of terbinafine in murine and porcine skin. To mimic fungal infection of skin, keratinized skin was induced in NC/Nga mice. In comparison with the marketed formulations, DA 5505 exhibited significantly better skin permeation. The flux, permeation coefficient, and enhancement ratio of terbinafine were remarkably increased by DA 5505 in comparison with the marketed formulations, and lag time was dramatically reduced. DA 5505 significantly increased cumulative terbinafine retention in viable skin layers in comparison with the marketed solution, suggesting enhanced efficacy. Furthermore, DA 5505 exhibited superior skin permeation in normal skin and keratinized skin. Thus, the DA 5505 formulation has the potential to effectively deliver terbinafine to superficial and deep cutaneous fungal infections.

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NC/Nga Mice: A Mouse Model for Atopic Dermatitis

Cited by 222 publications

References 10 publications

Anti-Allergic Effect of a Hot-Water Extract of Quince (Cydonia oblonga)

Anti-Allergic Effect of a Hot-Water Extract of Quince (Cydonia oblonga)

Spontaneous Development of Dermatitis in DS-Nh Mice under Specific Pathogen-Free Conditions.

DA 5505: A Novel Topical Formulation of Terbinafine That Enhances Skin Penetration and Retention

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