NDM-12, a Novel New Delhi Metallo-β-Lactamase Variant from a Carbapenem-Resistant Escherichia coli Clinical Isolate in Nepal

Tada, Tatsuya; Shrestha, Basudha; Miyoshi‐Akiyama, Tohru; Shimada, Kayo; Ohara, Hiroshi; Kirikae, Teruo; Pokhrel, Bharat Mani

doi:10.1128/aac.03355-14

Cited by 32 publications

(19 citation statements)

References 44 publications

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“…CP-CRE prevalence rates are approximately 18% in two large hospitals in Nepal (99, 101). The most common carbapenemase in Enterobacteriaceae is NDM (101,102), with two new variants (NDM-8 and -12) described in Nepal recently (103,104). No mcr-positive Enterobacteriaceae have been found in Nepal to date.…”

Section: South Asiamentioning

confidence: 99%

Carbapenem-Resistant Acinetobacter baumannii and Enterobacteriaceae in South and Southeast Asia

et al. 2017

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SUMMARY Carbapenem-resistant Gram-negative bacteria, in particular the Acinetobacter baumannii-calcoaceticus complex and Enterobacteriaceae, are escalating global public health threats. We review the epidemiology and prevalence of these carbapenem-resistant Gram-negative bacteria among countries in South and Southeast Asia, where the rates of resistance are some of the highest in the world. These countries house more than a third of the world's population, and several are also major medical tourism destinations. There are significant data gaps, and the almost universal lack of comprehensive surveillance programs that include molecular epidemiologic testing has made it difficult to understand the origins and extent of the problem in depth. A complex combination of factors such as inappropriate prescription of antibiotics, overstretched health systems, and international travel (including the phenomenon of medical tourism) probably led to the rapid rise and spread of these bacteria in hospitals in South and Southeast Asia. In India, Pakistan, and Vietnam, carbapenem-resistant Enterobacteriaceae have also been found in the environment and community, likely as a consequence of poor environmental hygiene and sanitation. Considerable political will and effort, including from countries outside these regions, are vital in order to reduce the prevalence of such bacteria in South and Southeast Asia and prevent their global spread.

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Section: South Asiamentioning

confidence: 99%

Carbapenem-Resistant Acinetobacter baumannii and Enterobacteriaceae in South and Southeast Asia

et al. 2017

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“…To determine the sizes of the plasmids harboring bla NDM s, plasmid DNAs in each isolate were extracted and digested with S1 nuclease. Pulsed-field gel electrophoresis (PFGE) and Southern hybridization were performed (10).…”

mentioning

confidence: 99%

Emergence of Various NDM-Type-Metallo-β-Lactamase-Producing Escherichia coli Clinical Isolates in Nepal

Shrestha

Tada

Shimada

et al. 2017

Antimicrob Agents Chemother

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“…The open reading frames (ORFs) of bla IMP-1 , bla , and bla were PCR amplified using the following primer sets: EcoRI-IMP-1-F (5=-GGGGAATTCATGAGCAAGTTATCTGTATTC-3=) and PstI-IMP-1-R (5=-AAACTGCAGTTAGTTGCTTGGTTTTGATGG-3=), EcoRI-IMP-15-F (5=-ATGAATTCATGAACAAGTTATCTGTATTCT-3=) and PstI-IMP-15-R (5=-ATCTGCAGTTAGTTACTTGGCAGTGAT GGT-3=), and EcoRI-IMP-26-F (5=-ATGAATTCATGAGCAAGTTATCT GTATTCT-3=) and PstI-IMP-26-R (5=-ATCTGCAGTTAGTTGCTTAG TTTTGATGGT-3=), respectively. The E. coli transformants expressing bla IMP-1 , bla , and bla IMP-26 were produced as previously described (22). The PCR products of each were digested with EcoRI and PstI and ligated into pHSG398 (TaKaRa Bio, Shiga, Japan).…”

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confidence: 99%

“…Recombinant IMP-1, IMP-15, and IMP-26 proteins were purified as described previously (22). The ␤-lactamase activities were monitored during the purification process using nitrocefin (Oxoid, Ltd., Basingstoke, United Kingdom).…”

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confidence: 99%

Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam

Tada

Nhung

Miyoshi‐Akiyama

et al. 2016

Antimicrob Agents Chemother

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Forty clinical isolates of multidrug-resistant Pseudomonas aeruginosa were obtained in a medical setting in Hanoi, Vietnam. Whole genomes of all 40 isolates were sequenced by MiSeq (Illumina), and phylogenic trees were constructed from the single nucleotide polymorphism concatemers. Of these 40 isolates, 24 (60.0%) harbored metallo-␤-lactamase-encoding genes, including bla IMP-15 , bla IMP-26 , bla IMP-51 , and/or bla NDM-1 . Of these 24 isolates, 12 harbored bla IMP-26 and belonged to sequence type 235 (ST235). Escherichia coli expressing bla IMP-26 was significantly more resistant to doripenem and meropenem than E. coli expressing bla IMP-1 and bla IMP-15 . IMP-26 showed higher catalytic activity against doripenem and meropenem than IMP-1 and against all carbapenems tested, including doripenem, imipenem, meropenem, and panipenem, than did IMP-15. These data suggest that clinical isolates of multidrug-resistant ST235 P. aeruginosa producing IMP-26 with increased carbapenem-hydrolyzing activities are spreading in medical settings in Vietnam.

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NDM-12, a Novel New Delhi Metallo-β-Lactamase Variant from a Carbapenem-Resistant Escherichia coli Clinical Isolate in Nepal

Cited by 32 publications

References 44 publications

Carbapenem-Resistant Acinetobacter baumannii and Enterobacteriaceae in South and Southeast Asia

Carbapenem-Resistant Acinetobacter baumannii and Enterobacteriaceae in South and Southeast Asia

Emergence of Various NDM-Type-Metallo-β-Lactamase-Producing Escherichia coli Clinical Isolates in Nepal

Multidrug-Resistant Sequence Type 235 Pseudomonas aeruginosa Clinical Isolates Producing IMP-26 with Increased Carbapenem-Hydrolyzing Activities in Vietnam

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