NDM-5 Carbapenemase-Encoding Gene in Multidrug-Resistant Clinical Isolates of Escherichia coli from Algeria

Sassi, Asma; Loucif, Lotfi; Gupta, Sushim K.; Dekhil, Mazouz; Chettibi, Houria; Rolain, Jean‐Marc

doi:10.1128/aac.02818-13

Cited by 58 publications

(40 citation statements)

References 11 publications

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“…Our study confirms the emergence of NDM-4-and NDM-5-producing bacteria in the African continent after their recent identification in Cameroon and Algeria, respectively (3,10). The worldwide emergence of NDM-4 and NDM-5 producers is quite alarming, particularly when the increased carbapenemase activity of NDM-4 and NDM-5, compared with that of NDM-1, is taken into account.…”

supporting

confidence: 71%

NDM-4- and NDM-5-Producing Klebsiella pneumoniae Coinfection in a 6-Month-Old Infant

Khalifa

Soliman

Ahmed

et al. 2016

Antimicrob Agents Chemother

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supporting

confidence: 71%

NDM-4- and NDM-5-Producing Klebsiella pneumoniae Coinfection in a 6-Month-Old Infant

Khalifa

Soliman

Ahmed

et al. 2016

Antimicrob Agents Chemother

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“…Interestingly, 1 isolate (Vir-14) was positive for all of the 4 tested ESBL and carbapenemases. Since the first description of NDM, several variants of the gene have been reported [31]. More recently, 7 NDM (1-7) variants were described from an Indian health care center [29].…”

Section: Discussionmentioning

confidence: 99%

Detection of Antibiotic Resistance Determinants and Their Transmissibility among Clinically Isolated Carbapenem-Resistant <b><i>Escherichia coli</i></b> from South India

et al. 2018

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Objectives: The aim of this study was to analyze the prevalence of the CTX-M, TEM, SHV, VIM, NDM, and OXA genes in carbapenemase-producing Escherichia coli and their transmissibility at a tertiary care hospital in south India. Materials and Methods: Twenty-one carbapenem-resistant E. coli (carbapenem-resistant Enterobacteriaceae; CRE) were collected from the Sri Sathya Sai Institute of Higher Medical Sciences (Puttaparthi India). Resistance to antibiotics was analyzed by Vitek-2, and the identity of the isolates was confirmed by 16S rDNA sequencing. RAPD and enterobacterial repetitive intergenic consensus (ERIC)-PCR were performed for molecular typing. Metallo-β-lactamase production was confirmed by a double disc synergy test. The presence of the extended-spectrum β-lactamases CTX-M, TEM, and SHV and of the carbapenemases NDM, VIM, and OXA was determined by PCR. Carbapenemase variants were further confirmed by sequencing. The transmissibility of the genes was tested by conjugation. Results: Twelve of the 21 (57%) carbapenem-resistant E. coli isolates were community acquired, indicating the spread of CRE in environmental samples. TEM and NDM-5 were found to be the major β-lactamases produced by the pathogens. OXA-181 was found in 5 of the isolates. All 21 isolates were found to harbor more than one of the tested β-lactamases, and all of the isolates were found to have the capacity to participate in conjugation; 15 of the transconjugants were found to have acquired the tested β-lactamases, substantiating their ability to be transferred to other strains of bacteria. Conclusion: Monitoring of community-acquired carbapenem-resistant bacteria is very important as the association of resistance determinants with mobile genetic elements would present a serious clinical challenge.

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“…The New Delhi metallo-␤-lactamase (NDM) was first described in 2008 (1) and has become one of the most widespread carbapenemases in the world (2)(3)(4). Meanwhile, since the recent discovery of the plasmid-mediated colistin resistance gene mcr-1 in China (5), several studies have confirmed its dissemination in different humans and animals (6)(7)(8)(9)(10).…”

mentioning

confidence: 99%

Emergence of NDM-5- and MCR-1-Producing Escherichia coli Clones ST648 and ST156 from a Single Muscovy Duck (Cairina moschata)

Yang

Feng

et al. 2016

Antimicrob Agents Chemother

117

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c Two Escherichia coli clones (sequence type 648 [ST648] and ST156) that coproduce NDM-5 and MCR-1 were detected from a single fowl in China. The bla NDM-5 gene was found on the two indistinguishable IncX3 plasmids from the two different E. coli isolates, whereas the mcr-1 gene was located on IncHI2 and IncI2 plasmids, respectively, suggesting that bla NDM-5 and mcr-1 have spread in avian intestinal flora. Also, the two strains harbor bla TEM-1 , bla CTX-M-55 , fosA3, and aac(6=)-Ib. The multiresistant E. coli strains (especially the epidemic clone ST648) might raise a potential threat to human health via food chain transmission. Carbapenem-resistant Enterobacteriaceae (CRE) are a global public health problem. The New Delhi metallo-␤-lactamase (NDM) was first described in 2008 (1) and has become one of the most widespread carbapenemases in the world (2-4). Meanwhile, since the recent discovery of the plasmid-mediated colistin resistance gene mcr-1 in China (5), several studies have confirmed its dissemination in different humans and animals (6-10). In addition, the coproduction of carbapenemase and MCR-1 was detected in a few bacteria (6,8,(11)(12)(13), which poses a serious concern to public health. Here, we report the first case of NDM-5-and MCR-1-producing Escherichia coli clones sequence type 648 (ST648) and ST156 from a single fowl.In May 2015, a rectal swab was collected from a 1-month-old Muscovy duck (Cairina moschata) with colibacillosis to study carbapenemase-encoding genes from animals in China. The diseased duck was sent to the veterinary clinical diagnosis laboratory in South China Agricultural University from a duck farm in Guangdong Province, China. Carbapenem-producing isolates were selected in MacConkey medium supplemented with meropenem (1 g/ml). Two E. coli isolates (NDM131 and NDM132) with diverse morphological characteristics were isolated and were identified by the Axima matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometer (Shimadzu-Biotech Corp., Kyoto, Japan).Antibiotic susceptibility testing was performed using the agar dilution method (14) and interpreted according to the Clinical and Laboratory Standards Institute guidelines (15). The EUCAST breakpoints for Enterobacteriaceae for colistin and tigecycline were applied (16).Both E. coli isolates (NDM131 and NDM132) were resistant to cefoxitin, ceftazidime, cefotaxime, imipenem, meropenem, ertapenem, gentamicin, tobramycin, ciprofloxacin, tetracycline, fosfomycin, trimethoprim-sulfamethoxazole, and colistin but susceptible to tigecycline (see Table S1 in the supplemental material). NDM131 was also resistant to aztreonam and amikacin. The double-disk synergy test and biochemical Carba NP test confirmed extended-spectrum ␤-lactamase (ESBL) and carbapenemase production in both isolates.PCR analyses were performed to confirm carbapenemase, ESBLs, plasmid-mediated AmpC cephalosporinase-encoding genes, 16S rRNA methyltransferase genes, and fosfomycin resistance genes. The complete coding sequence of the NDM g...

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NDM-5 Carbapenemase-Encoding Gene in Multidrug-Resistant Clinical Isolates of Escherichia coli from Algeria

Cited by 58 publications

References 11 publications

NDM-4- and NDM-5-Producing Klebsiella pneumoniae Coinfection in a 6-Month-Old Infant

NDM-4- and NDM-5-Producing Klebsiella pneumoniae Coinfection in a 6-Month-Old Infant

Detection of Antibiotic Resistance Determinants and Their Transmissibility among Clinically Isolated Carbapenem-Resistant <b><i>Escherichia coli</i></b> from South India

Emergence of NDM-5- and MCR-1-Producing Escherichia coli Clones ST648 and ST156 from a Single Muscovy Duck (Cairina moschata)

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