NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement

Willet, Alaina H.; DeWitt, Ashley K.; Beckley, Janel R.; Clifford, Dawn M.; Gould, Kathleen L.

doi:10.1016/j.cub.2019.01.075

Cited by 25 publications

(39 citation statements)

References 50 publications

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“…The SIN signal begins with the activity of the GTPase Spg1p and involves a regulatory GAP complex, several scaffolds and a linear cascade of three kinases (Cdc7p,Sid1p,and Sid2p), in order of their activation. Substrates of the Sid2p kinase are the phosphatase Clp1p/Flp1p (Mishra, Karagiannis, Sevugan, Singh, & Balasubramanian, 2005), the formin Cdc12p (Bohnert & Gould, 2012), the SIN scaffold Cdc11p (Feoktistova et al, 2012), the kinesin Klp2p (Mana-Capelli, McLean, Chen, Gould, & McCollum, 2012), the SAD kinase Cdr2p (Rincon et al, 2017), the anillin Mid1p (Willet, DeWitt, Beckley, Clifford, & Gould, 2019) and several others (Grallert, Connolly, Smith, Simanis, & Hagan, 2012;Gupta et al, 2013). Overexpression of Rho GTPase Rho1p, its GEFs Rgf1p and Rgf3p, as well as inactivation of Rho1p GAPs, partially rescues the lethality of sid2 mutants at a low restrictive temperature (Alcaide-Gavilan, Lahoz, Daga, & Jimenez, 2014;Jin, Zhou, Bimbo, Balasubramanian, & McCollum, 2006).…”

Section: Introductionmentioning

confidence: 99%

A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast

Edreira

Celador

Manjón

et al. 2020

eLife

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In fission yeast, the septation initiation network (SIN) ensures temporal coordination between actomyosin ring (CAR) constriction with membrane ingression and septum synthesis. However, questions remain about CAR regulation under stress conditions. We show that Rgf1p (Rho1p GEF), participates in a delay of cytokinesis under cell wall stress (blankophor, BP). BP did not interfere with CAR assembly or the rate of CAR constriction, but did delay the onset of constriction in the wild type cells but not in the rgf1Δ cells. This delay was also abolished in the absence of Pmk1p, the MAPK of the cell integrity pathway (CIP), leading to premature abscission and a multi-septated phenotype. Moreover, cytokinesis delay correlates with maintained SIN signaling and depends on the SIN to be achieved. Thus, we propose that the CIP participates in a checkpoint, capable of triggering a CAR constriction delay through the SIN pathway to ensure that cytokinesis terminates successfully.

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Section: Introductionmentioning

confidence: 99%

A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast

Edreira

Celador

Manjón

et al. 2020

eLife

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“…Plo1p phosphorylates residues within the first 100 amino acids of Mid1p to trigger Myosin II recruitment during contractile ring assembly [25]. Later at contractile ring constriction Sid1p phosphorylates Mid1p to facilitate its export from the cell cortex [7]. Future experiments will be aimed at unraveling how the Mid1p interactome is modified both by association with Vps4p and by phosphorylation by mitotic kinases.…”

Section: Discussionmentioning

confidence: 99%

Anillin/Mid1p interacts with the ECSRT-associated protein Vps4p and mitotic kinases to regulate cytokinesis in fission yeast

McInerny

Rezig

Gould

2019

Preprint

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26Cytokinesis is the final stage of the cell cycle which separates cellular constituents to 27 produce two daughter cells. Using Schizosaccharomyces pombe we have 28 investigated the role of various classes of proteins involved in this process. Central to 29 these is anillin/Mid1p which forms a ring-like structure at the cell equator that predicts 30 the site of cell separation through septation in fission yeast. Here we demonstrate a 31 direct physical interaction between Mid1p and the endosomal sorting complex 32 required for transport (ESCRT)-associated protein Vps4p. The interaction is essential 33 for cell viability, and Vps4p is required for the correct cellular localization of Mid1p. 34Furthermore, we show that Mid1p is phosphorylated by the aurora kinase Aurora A, 35that the interaction of mid1 and ark1 genes is essential for cell viability, and that 36Ark1p is also required for the correct cellular localization of Mid1p. We mapped the 37 sites of phosphorylation of Mid1p by Aurora A and the polo kinase Plk1 and 38 assessed their importance by mutational analysis. Mutational analysis revealed 39 S332, S523 and S531 to be required for Mid1p function and its interaction with 40Vps4p, Ark1p and Plo1p. Combined our data suggest a physical interaction between 41Mip1p and Vps4p important for cytokinesis, and identify phosphorylation of Mid1p by 42 aurora and polo kinases as being significant for this process. 43 44 Author summary 45Replication is a property of all living cells, with cell separation, so-called cytokinesis, 46 the final step in the process. A large number of proteins have been identified that are 47 complex required for transport (ESCRT) proteins and the anillin protein Mid1p. We 51 identify a direct physical interaction between the ESCRT protein Vps4 and 52 anillin/Mid1p, and explore how it regulates cytokinesis. Midp1 activity is shown to 53 controlled by the protein kinase Ark1p by direct phosphorylation, and this 54 phosphorylation is important for Mid1p function. These observations identify new 55 ways in which ESCRT and anillin/Mid1p control cell separation. 56 57

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“…Myp2 no es tan importante para la constricción como Myo2 (Palani et al 2017;Zambon et al 2017), pero contribuye a la estabilidad y constricción del anillo, especialmente bajo situaciones de estrés (O'Shaughnessy & Thiyagarajan 2018;Okada et al 2019). Finalmente, Mid1 se disocia del anillo antes de iniciar la constricción en un proceso mediado por la fosforilación por la kinasa Sid2 (Willet et al 2019). Tanto Mid1 como Cdr2 dejarían el anillo para eliminar la información posicional marcada en la célula madre que ya está en citoquinesis (ver apartado anterior).…”

Section: Maduración Del Carunclassified

“…Entre los sustratos de Sid2 están la fosfatasa Clp1 (Mishra et al 2005), la formina Cdc12 (Bohnert et al 2013), la proteína de anclaje Cdc11 (Feoktistova et al 2012), la kinesina Klp2 (Mana-Capelli et al 2012), la kinasa Cdr2 (Rincon et al 2017) y la anillina Mid1 (Willet et al 2019) entre otras (Grallert et al 2012;Gupta et al 2013). La ruta SIN participa en la condensación de los nodos durante el ensamblaje del CAR cooperando con Mid1, y asegura la estabilidad del anillo en…”

NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement

Abstract: Graphical Abstract Highlights d Anillin-like Mid1 is a substrate of the SIN kinase Sid2 d SIN signaling controls Mid1's membrane localization d Sid2 temporally regulates Cdr2-Mid1 association d Cdr2 and Mid1 are key SIN substrates for resetting cell division site placement

Cited by 25 publications

References 50 publications

A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast

A novel checkpoint pathway controls actomyosin ring constriction trigger in fission yeast

Anillin/Mid1p interacts with the ECSRT-associated protein Vps4p and mitotic kinases to regulate cytokinesis in fission yeast

Regulación de la citoquinesis por Rgf1 y la ruta de integridad

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