Near infrared emitting and biocompatible Yb3+-DNA complexes with dual responses to Cu2+ and Fe3+

Conditionally designed luminescent DNA crystals doped by Ln3+(Eu3+/Tb3+) complexes or fluorescent proteins with smart drug sensing property

“…The phosphate-modified crystals SC-5p and EC-5p were moved into the buffer using a cryoloop (Hampton Research) and washed in buffer (0.030 M sodium dimethyl arsonate, 0.055 M MgCl 2 , 0.050 M NaCl, 0.025 M HEPES (pH = 6)) for 10 min to remove the excess DNA strands. 42 After washing, the crystals were transferred to chemical ligation wells containing an equal mixture of EDC crosslinking agent and buffer. The ligation reaction was left to stand for 24 hours.…”