Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique

Argilés-Herrero, Rafael; Salvador-Herranz, Gustavo; Parker, Andrew G.; Zacarés, Mario; Fall, Assane Guèye; Gaye, Adji M.; Nawaz, Asif; Takáč, Peter; Vreysen, Marc J. B.; Beer, Chantel J. de

doi:10.1051/parasite/2023019

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…Pupae produced in the colony were collected daily and sorted by sex with a newly developed Near Infrared Pupae Sex Sorter (NIRPSS), preconditioned with the melanisation parameters set at T1 of 252, T2 of 0.10 and T3 of 10, 23–24 days post larviposition 68 . Male pupae were selected from the pupae classed as unmelanized when the unmelanized ratio (unmelanized pupae/total pupae sorted) was below 38%.…”

Section: Methodsmentioning

confidence: 99%

X-rays are as effective as gamma-rays for the sterilization of Glossina palpalis gambiensis Vanderplank, 1911 (Diptera: Glossinidae) for use in the sterile insect technique

Kaboré,

Nawaj,

Maiga

et al. 2023

Sci Rep

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An area-wide integrated pest management strategy with a sterile insect technique (SIT) component requires a radiation source for the sterilisation of male insects. Self-contained gamma irradiators, which were exclusively used in past SIT programmes, are now facing increasing constraints and challenges due to stringent regulations. As a potential alternative, new generation high output X-ray irradiators have been proposed. The feasibility of using X-ray irradiators was assessed by comparing the effects of both gamma- and X-ray irradiators on biological parameters of Glossina palpalis gambiensis (Vanderplank, 1911), that are important for SIT applications. The gamma irradiator Foss Model 812 and two X-ray irradiators, the Rad Source 2400 and the blood irradiator Raycell Mk2 were used. Glossina palpalis gambiensis males were exposed to radiation as pupae. A radiation dose of 110 Gy or above induced more than 97% sterility in females that mated with the irradiated males for all the irradiators. Adult emergence rate, flight propensity, survival and mating performance did not differ between gamma- and X-rays irradiators. These results suggest that irradiating pupae with a dose of 110 Gy is optimal for both gamma-and X-ray irradiators used in this study, to achieve a sterility of approximately 99%. Similar research on other tsetse species could gradually phase out the use of gamma-ray irradiators in favour of X-rays irradiators, especially for smaller SIT programmes.

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Section: Methodsmentioning

confidence: 99%

X-rays are as effective as gamma-rays for the sterilization of Glossina palpalis gambiensis Vanderplank, 1911 (Diptera: Glossinidae) for use in the sterile insect technique

Kaboré,

Nawaj,

Maiga

et al. 2023

Sci Rep

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Radiation dose fractionation and its potential hormetic effects on male Glossina palpalis gambiensis (Diptera: Glossinidae): a comparative study of reproductive and flight quality parameters

Kaboré,

Taqi,

Mkinga

et al. 2024

Parasite

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One of the most critical factors for implementing the sterile insect technique for the management of tsetse is the production of large quantities of highly competitive sterile males in the field. Several factors may influence the biological quality of sterile males, but optimizing the irradiation protocols to limit unwanted somatic cell damage could improve male performance. This study evaluated the effect of fractionation of gamma radiation doses on the fertility and flight quality of male Glossina palpalis gambiensis. Induced sterility was assessed by mating irradiated males with virgin fertile females. Flight quality was assessed using a standard protocol. The male flies were irradiated as pupae on day 23–27 post larviposition with 110 Gy, either in a single dose or in fractionations of 10 + 100 Gy and 50 + 60 Gy separated by 1-, 2- and 3-day intervals or 55 + 55 Gy separated by 4-, 8-, and 24-hour intervals. All treatments induced more than 90% sterility in females mated with irradiated males, as compared with untreated males. No significant differences were found in emergence rate or flight propensity between fractionated and single radiation doses, nor between the types of fractionations. Overall, the 50(D0) + 60(D1) Gy dose showed slightly higher induced sterility, flight propensity, and survival of males under feeding regime. Dose fractionation resulted in only small improvements with respect to flight propensity and survival, and this should be traded off with the required increase in labor that dose fractionation entails, especially in larger control programs.

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Rearing of Glossina morsitans morsitans tsetse flies for the sterile insect technique: evaluating the impact of irradiation and transportation during early and late-stage pupal development on the quality of emerging adults

Mirieri,

Uzel,

Parker

et al. 2024

Parasite

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Human African trypanosomiasis (HAT) and African animal trypanosomosis (AAT) are devastating diseases spread by tsetse flies (Glossina spp.), affecting humans and livestock, respectively. Current efforts to manage these diseases by eliminating the vector through the sterile insect technique (SIT) require transportation of irradiated late-stage tsetse pupae under chilling, which has been reported to reduce the biological quality of emerged flies. We therefore evaluated the impact of irradiation and transportation (including vibration and shock) on pupae at early-stage development (22 days of age) under ambient temperature and compared it to that on pupae at the late-stage development (29 days of age) under chilling, the current practice for tsetse in SIT programs. The quality of flies emerging from these transported pupae was assessed by their emergence rates, flight propensity, mating ability, insemination rates and survival rates (over ca. 100 days, and after specified shorter periods). Generally, flies emerging from the 22-day-old pupae had significantly (p < 0.05) higher values for the tested quality parameters, as compared to those emerging from 29-day-old pupae. Irradiation, transportation and the combination thereof significantly (p < 0.05) reduced all the tested quality parameters as compared with the untreated control within the 22-day-old pupae group. Further, vibration had a significant negative effect on the quality of flies, notwithstanding the age of the pupae. Irradiation and transportation of pupae at 22 days of age resulted in a higher proportion of flies of good biological quality as compared to those of 29 days of age, and hence may be considered for future SIT programs.

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Near-infrared imaging for automated tsetse pupae sex sorting in support of the sterile insect technique

Cited by 4 publications

References 35 publications

X-rays are as effective as gamma-rays for the sterilization of Glossina palpalis gambiensis Vanderplank, 1911 (Diptera: Glossinidae) for use in the sterile insect technique

X-rays are as effective as gamma-rays for the sterilization of Glossina palpalis gambiensis Vanderplank, 1911 (Diptera: Glossinidae) for use in the sterile insect technique

Radiation dose fractionation and its potential hormetic effects on male Glossina palpalis gambiensis (Diptera: Glossinidae): a comparative study of reproductive and flight quality parameters

Rearing of Glossina morsitans morsitans tsetse flies for the sterile insect technique: evaluating the impact of irradiation and transportation during early and late-stage pupal development on the quality of emerging adults

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