Near‐Infrared SERS Nanoprobes with Plasmonic Au/Ag Hollow‐Shell Assemblies for In Vivo Multiplex Detection

Abstract

For the effective application of surface‐enhanced Raman scattering (SERS) nanoprobes for in vivo targeting, the tissue transparency of the probe signals should be as high as it can be in order to increase detection sensitivity and signal reproducibility. Here, near‐infrared (NIR)‐sensitive SERS nanoprobes (NIR SERS dots) are demonstrated for in vivo multiplex detection. The NIR SERS dots consist of plasmonic Au/Ag hollow‐shell (HS) assemblies on the surface of silica nanospheres and simple aromatic Raman labels. The diameter of the HS interior is adjusted from 3 to 11 nm by varying the amount of Au3+ added, which results in a red‐shift of the plasmonic extinction of the Au/Ag nanoparticles toward the NIR (700–900 nm). The red‐shifted plasmonic extinction of NIR SERS dots causes enhanced SERS signals in the NIR optical window where endogenous tissue absorption coefficients are more than two orders of magnitude lower than those for ultraviolet and visible light. The signals from NIR SERS dots are detectable from 8‐mm deep in animal tissues. Three kinds of NIR SERS dots, which are injected into live animal tissues, produce strong SERS signals from deep tissues without spectral overlap, demonstrating their potential for in vivo multiplex detection of specific target molecules.

Mesoporous Silica‐Coated Plasmonic Nanostructures for Surface‐Enhanced Raman Scattering Detection and Photothermal Therapy

“…Furthermore, in order to meet the sensitivity and depth for detection of in vivo, the near‐infrared surface‐enhanced Raman scattering (NIR SERS) is vital due to the high transparency of soft tissues than visible light . Therefore, we also utilize the obtained Au‐Ag@mSiO 2 nanocarrier with the SPR of 790 nm (reacted with 1.0 mL HAuCl 4 , Figure B‐1.0) as a nanoprobe to investigate the NIR SERS detection.…”

Markerfreie molekulare Bildgebung biologischer Zellen und Gewebe durch lineare und nichtlineare Raman‐spektroskopische Ansätze

“…AuNPs wurden zu Dimeren unter Verwendung eines Dithiol‐Verbinders als Reportermolekül zusammengebaut, und die Peptidfunktionalisierung verbesserte ihre Stabilität sogar bei Inkubation mit lebenden Zellen . Um die Empfindlichkeit zu erhöhen, wurden Au/Ag‐Hohlschalen‐Anordnungen auf der Oberfläche von Siliciumdioxid‐Nanopartikeln hergestellt . Diese Konstrukte wurden mit einem aromatischen Raman‐Marker, z.…”

“…Zusätzlich zu den Multiplex‐Fähigkeiten von SERS‐Labeln, die durch die Verwendung verschiedener Raman‐Reportermoleküle erreicht werden, erlangten multimodale Ansätze, die zwei oder mehr Auslesemodalitäten verwenden, viel Aufmerksamkeit von Forschern für die Zell‐ und Gewebebildgebung.…”

Surface-enhanced Raman scattering (SERS)-active gold nanochains for multiplex detection and photodynamic therapy of cancer

“…These results corresponded well with the plasmon absorption of the UV-visible spectra ( Figure 3B). The surface enhancement factors (EF) for HA-HCA n -Au-NPT were roughly estimated by comparing the peak intensity at 1375 cm -1 of HA-HCA n -Au-NPT and free NPT [33][34][35]. As shown in Table 1, EF increased with values of 2.7×10 5 , 2.2×10 6 , 5.7×10 7 , and 1.8×10 8 as the length of the HA-HCA n -Au-NPT nanochains increased.…”