Near-infrared spectroscopy and imaging in protein research

Ishigaki, Mika; Ozaki, Yukihiro

doi:10.1016/b978-0-12-818610-7.00006-2

Cited by 18 publications

(29 citation statements)

References 89 publications

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“…NIR spectral bands of proteins carry essential information that enables monitoring complex biological processes in vivo, such as embryonic development [89,90]. In particular, NIR bands are sensitive to hydrogen bonding and thus, to the properties of such molecules in their native aqueous environment as well [3].…”

Section: Investigations Into Structure Properties and Interactions mentioning

confidence: 99%

Near-Infrared Spectroscopy in Bio-Applications

2020

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Near-infrared (NIR) spectroscopy occupies a specific spot across the field of bioscience and related disciplines. Its characteristics and application potential differs from infrared (IR) or Raman spectroscopy. This vibrational spectroscopy technique elucidates molecular information from the examined sample by measuring absorption bands resulting from overtones and combination excitations. Recent decades brought significant progress in the instrumentation (e.g., miniaturized spectrometers) and spectral analysis methods (e.g., spectral image processing and analysis, quantum chemical calculation of NIR spectra), which made notable impact on its applicability. This review aims to present NIR spectroscopy as a matured technique, yet with great potential for further advances in several directions throughout broadly understood bio-applications. Its practical value is critically assessed and compared with competing techniques. Attention is given to link the bio-application potential of NIR spectroscopy with its fundamental characteristics and principal features of NIR spectra.

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Section: Investigations Into Structure Properties and Interactions mentioning

confidence: 99%

Near-Infrared Spectroscopy in Bio-Applications

2020

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“…As already mentioned above, Wu et al carried out a protein hydration study of human serum albumin by 2D-NIR COS spectroscopy. 13 It was found from the 2D-COS study that the protein unfolding occurs almost in parallel with the change in the protein hydration. Recently, Ishigaki et al investigated the relationship between bioactivity originated from embryogenesis and water structure in Japanese medaka fish (Oryzias latipes) eggs using NIR imaging.…”

Section: Applications Of Nir Spectroscopy To Life Sciencementioning

confidence: 99%

“…Wu reported the usefulness of 2DNIR spectroscopy in monitoring subtle changes in protein dynamics, especially hydration. 13 We also proposed several new algorithms for 2D-COS.…”

Section: When Did Serendipity Work In Our Minds? -A Long Way Of Nir R...mentioning

confidence: 99%

“…Recently, Ishigaki et al investigated the relationship between bioactivity originated from embryogenesis and water structure in Japanese medaka fish (Oryzias latipes) eggs using NIR imaging. 13 The variations of yolk components were analyzed for four kinds of egg samples: (a) eggs that were activated by fertilization and three kinds of nonactivated eggs where embryogenesis was stopped or not started by (b) culturing under cold temperatures, (c) instant freezing, or (d) the lack of fertilization. To explore the yolk component differences between the activated and non-activated eggs, PCA was performed to the dataset of NIR second derivatives.…”

Section: Applications Of Nir Spectroscopy To Life Sciencementioning

confidence: 99%

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NIR spectroscopy – What a wonderful world!

Ozaki

2022

NIR news

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This review article for the Karl Norris Award Special Issue consists of four parts. First, the unique history of NIR spectroscopy is described briefly. Then, I discuss the main topic, “what a wonderful world – uniqueness and versatility of NIR spectroscopy.” Next, I introduce our NIR studies for the last 35 years – When did serendipity work in our minds? – a long way of NIR research in the Ozaki group. The investigations on spectral analysis, applications of NIR spectroscopy to physical chemistry, those to life science and NIR imaging studies are reported. Finally, the perspective of NIR spectroscopy is discussed briefly. Through this review, I emphasize on the importance of serendipity in the research.

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“…This technique is primarily recognized in the applications to analytical chemistry [4] as a rapid, cost-efficient, simple, and non-destructive technique with nearly no sample preparation required, which appears to be a very attractive and useful alternative tool for qualitative and quantitative analyses in the agriculture [5,6], food [7,8], chemical [9,10], and pharmaceutical industries [11]. However, it is also met with an increasing importance in bioscience [12,13], medicine [14,15], and various applications related to hyperspectral imaging as well [16]. This method appears also useful in physical chemistry by delivering unique information on the molecular structure, hydrogen bonding, interactions, and dynamics [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Anharmonic DFT Study of Near-Infrared Spectra of Caffeine: Vibrational Analysis of the Second Overtones and Ternary Combinations

et al. 2021

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Anharmonic quantum chemical calculations were employed to simulate and interpret a near-infrared (NIR) spectrum of caffeine. First and second overtones, as well as binary and ternary combination bands, were obtained, accurately reproducing the lineshape of the experimental spectrum in the region of 10,000–4000 cm−1 (1000–2500 nm). The calculations enabled performing a detailed analysis of NIR spectra of caffeine, including weak bands due to the second overtones and ternary combinations. A highly convoluted nature of NIR spectrum of caffeine was unveiled, with numerous overlapping bands found beneath the observed spectral lineshape. To properly reflect that intrinsic complexity, the band assignments were provided in the form of heat maps presenting the contributions to the NIR spectrum from various kinds of vibrational transitions. These contributions were also quantitatively assessed in terms of the integral intensities. It was found that the combination bands provide the decisively dominant contributions to the NIR spectrum of caffeine. The first overtones gain significant importance between 6500–5500 cm−1, while the second overtones are meaningful in the higher wavenumber regions, particularly in the 10,000–7000 cm−1 region. The obtained detailed band assignments enabled deep interpretation of the absorption regions of caffeine identified in the literature as meaningful for analytical applications of NIR spectroscopy focused on quantitative analysis of caffeine content in drugs and natural products.

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Near-infrared spectroscopy and imaging in protein research

Cited by 18 publications

References 89 publications

Near-Infrared Spectroscopy in Bio-Applications

Near-Infrared Spectroscopy in Bio-Applications

NIR spectroscopy – What a wonderful world!

Anharmonic DFT Study of Near-Infrared Spectra of Caffeine: Vibrational Analysis of the Second Overtones and Ternary Combinations

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