Near perfect classification of cardiac biomarker Troponin-I in human serum assisted by SnS2-CNT composite, explainable ML, and operating-voltage-selection-algorithm

Device-Physics Realization of ZnO–MWCNT Nanostructure-Based Field-Effect Biosensor for Ultrasensitive Simultaneous Genomic Detection of Foodborne Pathogens

“…The –SH end of the MSA got anchored on the ZnO matrix by the well-known metal–thiol bond when treated with 1.5 μL of 10 mM MSA-DI water mixture. Following that, the equimolar composition of EDC-NHS cross-linking chemistry was utilized to activate the functionality of the carboxylic (−COOH) groups, present on the other end of the thiol . Subsequently, 1.5 μL of each food-pathogen probe (100 ng/μL in 0.1 molar PBS; pH 7.4) was introduced to achieve the amide (−CONH)-bonded covalent linkage of the bio receptor on top of the sensor surface.…”

“…Following that, the equimolar composition of EDC-NHS cross-linking chemistry was utilized to activate the functionality of the carboxylic (−COOH) groups, present on the other end of the thiol. 17 Subsequently, 1.5 μL of each foodpathogen probe (100 ng/μL in 0.1 molar PBS; pH 7.4) was introduced to achieve the amide (−CONH)-bonded covalent linkage of the bio receptor on top of the sensor surface. To block the unbounded surface groups, the devices were treated with an appropriate mixture of BSA in PBS and incubated at 37 °C for half an hour.…”

“…However, the underlying device-physics still remained unclear, thereby limiting the immense possibility to uncover key advances on better design of the sensors and new target detection strategies . Furthermore, the fundamentals of these biosensors have been given less priority over the application and thus, the principle behind the enhancement or reduction in the electrical detection signal in response to the target analyte endured in the dark. , Hence, it becomes necessary to understand the principle of operation of such sensor systems by investigating the impact of various chemical modifications on the electrical sensing parameters. Realizing this fact, this work illustrates the device physics of the field-effect-based electrical transduction principles relying on the energy band diagram and surface potential profile relating the current–voltage characteristics of a μ-IDE-based biosensor.…”

“…Hence, systematic investigation is sought for the perfect choice of the operating voltage of the biosensor devices, which is proven to be handy for extracting the most significant information from the experimental data. Toward this, an “operating–voltage–selection-algorithm” have been discussed in our previous work, modified here to eliminate computational requirements without compromising the performance . The behavior of such devices would mainly depend on the type of the charge carrier (p or n-type conduction) accounting for the accumulation or depletion, responding to the surface potential profile and the changes in the energy band diagram. ,, However, the biosensor literature largely deferred from the systematic investigation of this enhancement or reduction of current signal response, and there have been many proposals, such as electrostatic gating, , modulation of carrier mobility, Schottky barrier effect, gate-coupling etc.…”

Two-Dimensional Cs2AgBiBr6-Based Biosensor for Selective and Sensitive Detection of Cardiac Biomarker Troponin I

“…7 Elevated levels of cTnI can be detected in the blood within 2−6 h after the onset of AMI, with the concentration of cTnI reaching a maximum within 30 h. 8 Because increasing, compelling evidence revealed that AMI is extremely time critical, biosensors with a short turnaround time and highsensitivity are required for the early diagnosis of AMI. 9,10 Inspired by graphene and transition metal dichalcogenides (TMDs), the ever-increasing exploration of two-dimensional (2D) layered materials has been driven by their exceptional electronic, optical, physical, and chemical properties, making them highly desirable for various research fields. 11,12 In the past decade, halide perovskite materials have attracted increasing attention owing to their superior optical and electronic properties, encompassing suitable electrical bandgaps, outstanding carrier mobilities, intense photoluminescence (PL), and a relatively low processing temperature.…”