Needle-free immunization using a solid-in-oil nanodispersion enhanced by a skin-permeable oligoarginine peptide

“…Therefore, the S/O nanodispersions composed of smaller drug‐surfactant ratios induced higher antigen‐specific antibody responses . Encapsulation of strong polyelectrolytes facilitates particle disintegration, while other polymers such as hyaluronic acid are used to reinforce the particle stability .…”

Section: Development Of the S/o Nanodispersionsmentioning

confidence: 99%

“…OVA was more efficiently delivered to APCs when one of the skin‐penetrating peptides, hexa‐arginine, was co‐encapsulated in the S/O nanoparticles . However, how to repair the reduced barrier function of the skin and protect the body from infections is still controversial, especially after repeated drug administration.…”

Section: Application Of S/o Nanodispersion Systemsmentioning

confidence: 99%

Solid‐in‐oil nanodispersions for transdermal drug delivery systems

Kitaoka

Wakabayashi

Kamiya

et al. 2016

Biotechnology Journal

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Transdermal administration of drugs has advantages over conventional oral administration or administration using injection equipment. The route of administration reduces the opportunity for drug evacuation before systemic circulation, and enables long‐lasting drug administration at a modest body concentration. In addition, the skin is an attractive route for vaccination, because there are many immune cells in the skin. Recently, solid‐in‐oil nanodisperison (S/O) technique has demonstrated to deliver cosmetic and pharmaceutical bioactives efficiently through the skin. S/O nanodispersions are nanosized drug carriers designed to overcome the skin barrier. This review discusses the rationale for preparation of efficient and stable S/O nanodispersions, as well as application examples in cosmetic and pharmaceutical materials including vaccines. Drug administration using a patch is user‐friendly, and may improve patient compliance. The technique is a potent transcutaneous immunization method without needles.

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“…The production of OVA-specific serum IgG was evaluated using an enzyme-linked immunosorbent assay (ELISA), as described previously. 16 The antibody titer was defined by the dilution factor at which the optical density of the sample was equal to that of a non-immunized sample. To analyze cytokine secretion in splenocyte cultures, mice were sacrificed and their spleens were excised one week after the second immunization.…”

Section: Analysis Of Immune Responsementioning

confidence: 99%

Transcutaneous immunization against cancer using solid-in-oil nanodispersions

et al. 2015

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Transcutaneous immunization is a novel, non-invasive alternative to conventional immunization by injection. Skin immunocompetence comprised of abundant antigen-presenting cells in the epidermal and dermal layers of the skin can provide an effective tool for transcutaneous immunization, whereas the outermost, hydrophobic layer of skin, the stratum corneum, hinders penetration of antigens into the skin. To realize effective transcutaneous delivery of antigens, we have developed a solid-in-oil (S/O) technique that produces an oil dispersion of hydrophilic biomolecules. In this study, we applied the S/O nanodispersions in transcutaneous immunization to induce cancer immunity. The topical application of S/O nanodispersions bearing ovalbumin (OVA) as a model cancer-antigen allowed the penetration of OVA into the deep dermis region of the skin by intercellular and transfollicular pathways. Inhibition of OVA-bearing tumor growth and the production of cytokines responsible for cellular immunity was achieved, demonstrating the applicability of S/O nanodispersions to the induction of cancer immunity.

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Needle-free immunization using a solid-in-oil nanodispersion enhanced by a skin-permeable oligoarginine peptide

Cited by 18 publications

References 29 publications

Synthetic Nanoparticles for Vaccines and Immunotherapy

Synthetic Nanoparticles for Vaccines and Immunotherapy

Solid‐in‐oil nanodispersions for transdermal drug delivery systems

Transcutaneous immunization against cancer using solid-in-oil nanodispersions

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