Needle-free jet injection of small doses of Japanese encephalitis DNA and inactivated vaccine mixture induces neutralizing antibodies in miniature pigs and protects against fetal death and mummification in pregnant sows

Imoto, Jun-ichi; Ishikawa, Takahiro; Yamanaka, Akira; Konishi, Misako; Murakami, Kenji; Shibahara, Tomoyuki; Kubo, Michinori; Lim, Chang‐Kweng; Hamano, Masaaki; Takasaki, Tomohiko; Kurane, Ichiro; Udagawa, Haruhide; Mukuta, Yoshihiro; Konishi, Eiji

doi:10.1016/j.vaccine.2010.09.008

Cited by 18 publications

(9 citation statements)

References 46 publications

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“…This was comparable to the study of Sasaki and colleagues in which the pigs were viremic from day 1 to 4 pi using mosquito bite and subcutaneous injection [ 23 ]. Other studies also found a viremia of 1–3 days duration occurring between day 1 and 5 pi [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 73%

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Japanese encephalitis virus tropism in experimentally infected pigs

Ricklin¹,

García-Nicolás²,

Brechbühl³

et al. 2016

Vet Res

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Pigs are considered to be the main amplifying host for Japanese encephalitis virus (JEV), and their infection can correlate with human cases of disease. Despite their importance in the ecology of the virus as it relates to human cases of encephalitis, the pathogenesis of JEV in pigs remains obscure. In the present study, the localization and kinetics of virus replication were investigated in various tissues after experimental intravenous infection of pigs. The data demonstrate a rapid and broad spreading of the virus to the central nervous system (CNS) and various other organs. A particular tropism of JEV in pigs not only to the CNS but also for secondary lymphoid tissue, in particular the tonsils with the overall highest viral loads, was observed. In this organ, even 11 days post infection, the latest time point of the experiment, no apparent decrease in viral RNA loads and live virus was found despite the presence of a neutralizing antibody response. This was also well beyond the clinical and viremic phase. These results are of significance for the pathogenesis of JEV, and call for further experimental studies focusing on the cellular source and duration of virus replication in pigs.

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Section: Discussionmentioning

confidence: 73%

“…Previous studies indicate that neutralizing antibodies are important for protection against JEV with a protective neutralizing titer of 10, in both humans and in pigs [ 25 , 41 – 43 ]. Our study confirms that the immune system of pigs efficiently and rapidly reacts to JEV infection.…”

Section: Discussionmentioning

confidence: 99%

Japanese encephalitis virus tropism in experimentally infected pigs

Ricklin¹,

García-Nicolás²,

Brechbühl³

et al. 2016

Vet Res

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“…In this study, we demonstrated that immunization of pigs with a TRIP/JEV vector expressing JEV VLPs is particularly efficient at priming antigen-specific humoral immunity and triggers neutralizing antibody responses against the genotypes 1, 3, and 5 of JEV. The production of virus neutralizing antibodies is critical to protection against JEV infection in pigs [ 50 ] and a titer at least 1:10 is indicative of protective humoral immunity [ 51 ]. The titers of neutralizing antibodies elicited by the lentiviral TRIP/JEV.prME vector are sufficient to confer protection in domestic pigs against different genotypes of JEV and this could be of a great utility in endemic regions where more than one genotype circulates.…”

Section: Discussionmentioning

confidence: 99%

A Lentiviral Vector Expressing Japanese Encephalitis Virus-like Particles Elicits Broad Neutralizing Antibody Response in Pigs

Wispelaere

Ricklin²,

Souque

et al. 2015

PLoS Negl Trop Dis

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BackgroundJapanese encephalitis virus (JEV) is the major cause of viral encephalitis in Southeast Asia. Vaccination of domestic pigs has been suggested as a “one health” strategy to reduce viral disease transmission to humans. The efficiency of two lentiviral TRIP/JEV vectors expressing the JEV envelope prM and E glycoproteins at eliciting protective humoral response was assessed in a mouse model and piglets.Methodology/Principal FindingsA gene encoding the envelope proteins prM and E from a genotype 3 JEV strain was inserted into a lentiviral TRIP vector. Two lentiviral vectors TRIP/JEV were generated, each expressing the prM signal peptide followed by the prM protein and the E glycoprotein, the latter being expressed either in its native form or lacking its two C-terminal transmembrane domains. In vitro transduction of cells with the TRIP/JEV vector expressing the native prM and E resulted in the efficient secretion of virus-like particles of Japanese encephalitis virus. Immunization of BALB/c mice with TRIP/JEV vectors resulted in the production of IgGs against Japanese encephalitis virus, and the injection of a second dose one month after the prime injection greatly boosted antibody titers. The TRIP/JEV vectors elicited neutralizing antibodies against JEV strains belonging to genotypes 1, 3, and 5. Immunization of piglets with two doses of the lentiviral vector expressing JEV virus-like particles led to high titers of anti-JEV antibodies, that had efficient neutralizing activity regardless of the JEV genotype tested.Conclusions/SignificanceImmunization of pigs with the lentiviral vector expressing JEV virus-like particles is particularly efficient to prime antigen-specific humoral immunity and trigger neutralizing antibody responses against JEV genotypes 1, 3, and 5. The titers of neutralizing antibodies elicited by the TRIP/JEV vector are sufficient to confer protection in domestic pigs against different genotypes of JEV and this could be of a great utility in endemic regions where more than one genotype is circulating.

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“…150,151 Jet injectors have also been used for a wide variety of therapeutic drugs. [152][153][154][155][156][157][158][159][160][161][162][163][164][165][166][167][168][169][170][171] In recent years, the devices have been used to administer antigens to humans and animal models for a variety of investigational vaccines, including dengue, [172][173][174][175] herpes simplex type 2, 176 HIV/AIDS, [177][178][179] Japanese encephalitis, 180 malaria, 181 and melanoma. 182 A wide variety of investigational recombinant nucleic acid vaccines are being delivered in preclinical and clinical trials using various jet injectors.…”

Section: Hybrid Reconstitution Technologiesmentioning

confidence: 99%