Nanomaterials (NMs) developed using biomolecules display numerous advantages which attract the science community to explore them for a wide range of applications. In this line, bio-scaffolds are studied as templates to form nano-bio heterojunctions in the nano confined materials. With the high flexibility of biomediated NMs, it is possible to develop desired size and shape selective NMs. Such bio-based NMs have great benefits in wide areas including catalysis, sensors and energy related applications particularly, electrocatalysis, supercapacitor, batteries etc. The viability of bio-scaffolds in developing metal superstructures makes them better choice in the medicinal fields. This book chapter mainly focused on the advantageous and challenges of bioinspired NMs in the medicinal field, particularly in drug delivery systems. Moreover, the synthetic methods such as enzyme catalyzed wet-chemical route, photo-irradiation and incubation methods were also discussed in detail. Also, this chapter gives a better understanding to the readers about the development of new nano-bio heterojunctions for medicine, energy and environmental fields. Moreover, the morphological features of nano-bio interactions at nanoscale level show predominant activity particularly in Surface Enhanced Raman Scattering (SERS) and sensor applications. With the knowledge gained from this chapter, in futuristic, one can go for the development of new metal nanostructures with different bio-scaffolds such as microorganisms, viruses, DNA and protein to mainstream applications for the medicinal fields.