Neferine isolated from Nelumbo nucifera enhances anti-cancer activities in Hep3B cells: Molecular mechanisms of cell cycle arrest, ER stress induced apoptosis and anti-angiogenic response

Yoon, Jinsoo; Kim, Hwa-Mi; Yadunandam, Anandam Kasin; Kim, Nan‐Hee; Jung, Hyun‐Ah; Choi, Jong‐Yun; Kim, Chi-Yeon; Kim, Gun‐Do

doi:10.1016/j.phymed.2013.03.024

Cited by 78 publications

(48 citation statements)

References 38 publications

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“…Neferine, a major bisbenzylisoquinoline alkaloid derived from the embryos of Nelumbo nucifera, has been reported a few physiological activities. However, the mechanisms of anticancer effects are not well understood and its detailed activities on Hep3B cells have not been determined 19 .…”

Section: Anticancer Effectsmentioning

confidence: 99%

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2016

IJPSR

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objectives: Nelumbo nucifera Gaertn (Nelumbonaceae) known by numerous common names including Indian lotus, sacred lotus, bean of India, or simply lotus is one of the most important medicinal plants. In this review there is discussion about therapeutic potential of the different parts of the plant (leaves, seeds, flower, and rhizome) which can be used in traditional system of medicine. This review also describes various compounds isolated from different parts of the plant and therapeutic benefits derived from those phytocontituents. The aim of this review is to summarize the pharmacological, phytochemical and therapeutical knowledge of Nelumbo nucifera Gaertn. Key findings: The pharmacological studies have shown that N. nucifera possesses various pharmacological activities like anti-ischemic, antioxidant, anticancer, antiviral, antiobesity, lipolytic, hypocholestemic, antipyretic, hepatoprotective, hypoglycemic, antidiarrhoeal, antifungal, antibacterial, anti-inflammatory and diuretic activities. Several different types of compounds have been derived from this plant belonging to different chemical groups, including alkaloids, flavonoids, glycosides triterpenoid, etc., with own therapeutic impact. So the pharmacological effects and various active ingredients of different parts of N. nucifera are well understood. Summary: This review will facilitate to gain all about the past scientific research and will provide the necessary information about the enormous pharmacological activities of Nelumbo nucifera Gaertn which will insist the researchers for future research to protect human beings from various types of diseases and may serves as a natural gold for the promotion of mankind.

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Section: Anticancer Effectsmentioning

confidence: 99%

Untitled

2016

IJPSR

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“…In hepatocellular carcinoma (Hep3B) cells, neferine downregulated the dephosphorlyation of cdc2 and expression of c-Myc, cyclin D1, D3, CDK4, and E2F-1 proteins; this likely forms the molecular basis of the cell cycle arrest in Hep3B cells (Yoon et al 2013). In the same study, neferine also induced endoplasmic reticulum stress and apoptosis in the Hep3B cells.…”

Section: Nelumbo Nuciferamentioning

confidence: 99%

Edible freshwater macrophytes: a source of anticancer and antioxidative natural products—a mini-review

et al. 2015

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Edible freshwater macrophytes (EFM) are edible, macroscopic freshwater plants, many of which are also used as traditional/folk medicine. This minireview highlights phytochemical and pharmacological evidence pertaining to anticancer and antioxidative natural products derived from EFM, with special attention to Centella asiatica (Indian pennywort), Nelumbo nucifera (sacred lotus), Nasturtium officinale (watercress), Ipomoea aquatica (water spinach) and Ludwigia adscendens (water primrose). Current knowledge gaps and further research opportunities are also discussed. EFM is a promising source of anticancer and antioxidative natural products which warrants more extensive exploration. More research is needed before such natural products can be exploited for application in food and medicine.

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“…After reaching 80% confluence, they were harvested with 0.25% trypsin-0.02% EDTA solution and seeded in 96-well plate at a density of 1.0 Â 10 4 cells/well. The mock cells were exposed to the designated concentrations (5,10,15,20,30,40 and 80 lM) of neferine. MDCK-hCYP3A4 cells were incubated with vehicle or L-buthionine sulfoximine (BSO) (final concentration: 50 lM), an inhibitor of c-glutamylcysteine synthetase critical for biosynthesis of GSH or N-acetylcysteine (NAC) (final concentration: 5 mM), oxidation scavenger, prior to the exposure to neferine.…”

Section: Cell Culture and Cytotoxicity Assaymentioning

confidence: 99%

“…In fact, apoptosis induction by various cytotoxic anticancer agents has been one of the most effective methods for cancer therapy, and neferine-induced reactive oxygen species generation leads to caspase-dependent apoptosis in HepG2 cells [9]. Similarly, neferine exhibited cytotoxicity against HCC Hep3B cells through endoplasmic reticulum (ER) stress-induced apoptosis [10]. Furthermore, the potent growth-inhibitory effect of neferine on human osteosarcoma cells through promotion of p38 mitogenactivated protein kinase (MAPK)-mediated p21 stabilization was the first proof of its direct anti-tumor effect [11].…”

Section: Introductionmentioning

confidence: 99%