Neisseria weixii sp. nov., isolated from rectal contents of Tibetan Plateau pika (Ochotona curzoniae)

Gui, Zhilun; Yang, Jing; Lai, Xin‐He; Jin, Dong; Pu, Ji; Bai, Xiangning; Xiong, Yanwen; Ren, Zhihong; Luo, Xu; Huang, Ying; Lu, Shan; Xu, Jianguo

doi:10.1099/ijsem.0.003466

Cited by 15 publications

(12 citation statements)

References 27 publications

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“…If any region B or C sequences were acquired by the meningococcus by descent, they could not be identified in these sequence analyses ( Figure 4). The novel species N. weixii 48 was identified as a possible candidate ( Figure 3 and Figure 4), described as being isolated from the Tibetan Plateau Pika ( Ochotona curzoniae ) in the Qinghai Province, China (GenBank accession CP023429.1). This isolate contained sequences homologous to the N. animalis putative region A between its region C and region D homologues ( Figure 1B).…”

Section: Discussionmentioning

confidence: 99%

Neisseria meningitidis has acquired sequences within the capsule locus by horizontal genetic transfer

2019

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Background: Expression of a capsule from one of serogroups A, B, C, W, X or Y is usually required for Neisseria meningitidis (Nme) to cause invasive meningococcal disease. The capsule is encoded by the capsule locus, cps, which is proposed to have been acquired by a formerly capsule null organism by horizontal genetic transfer (HGT) from another species. Following identification of putative capsule genes in non-pathogenic Neisseria species, this hypothesis is re-examined. Methods: Whole genome sequence data from Neisseria species, including Nme genomes from a diverse range of clonal complexes and capsule genogroups, and non-Neisseria species, were obtained from PubMLST and GenBank. Sequence alignments of genes from the meningococcal cps, and predicted orthologues in other species, were analysed using Neighbor-nets, BOOTSCANing and maximum likelihood phylogenies. Results: The meningococcal cps was highly mosaic within regions B, C and D. A subset of sequences within regions B and C were phylogenetically nested within homologous sequences belonging to N. subflava, consistent with HGT event in which N. subflava was the donor. In the cps of 23/39 isolates, the two copies of region D were highly divergent, with rfbABC’ sequences being more closely related to predicted orthologues in the proposed species N. weixii (GenBank accession number CP023429.1) than the same genes in Nme isolates lacking a capsule. There was also evidence of mosaicism in the rfbABC’ sequences of the remaining 16 isolates, as well as rfbABC from many isolates. Conclusions: Data are consistent with the en bloc acquisition of cps in meningococci from N. subflava, followed by further recombination events with other Neisseria species. Nevertheless, the data cannot refute an alternative model, in which native meningococcal capsule existed prior to undergoing HGT with N. subflava and other species. Within-genus recombination events may have given rise to the diversity of meningococcal capsule serogroups.

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Section: Discussionmentioning

confidence: 99%

Neisseria meningitidis has acquired sequences within the capsule locus by horizontal genetic transfer

2019

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“…Members of the genus Arthrobacter were isolated from a variety of sources, including animals [6,7], cheese [8], glaciers [9,10], humans [11][12][13], sediments [14] and soil [15]. While investigating the microbiota of the Tibetan Plateau wildlife in PR China, we recently isolated some novel bacterial species from plateau pika (Ochotona curzoniae) and Tibetan wild ass (Equus kiang), including Actinomyces gaoshouyii [16], Neisseria chenwenguii [17], Neisseria weixii [18] and Norcardoides yefusunii [19]. Both yellow in colour, strain 785 T was isolated from the intestinal content of a plateau pika and strain 208 was from faeces of a Tibetan wild ass.…”

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confidence: 99%

“…Coloured pink, strains J391 T and J915, were isolated from droppings of ruddy shelduck (Tadorna ferruginea). The droppings samples of ruddy shelducks were freshly collected in 5 ml sterile tubes immediately after the birds flew away at different geographical locations (91° 48′ 22″ E, 27° 54′ 23″ N and 91° 58′ 53″ E, 27° 59′ 16″ N) of Cona County of Tibet and those of plateau pika and Tibetan wild ass were collected, transported and handled as described in our previous studies [16][17][18][19].…”

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confidence: 99%

Arthrobacter yangruifuii sp. nov. and Arthrobacter zhaoguopingii sp. nov., two new members of the genus Arthrobacter

Tao

Yang

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Four unknown strains belonging to the genus Arthrobacter were isolated from plateau wildlife on the Qinghai–Tibet Plateau of PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four isolates were separated into two clusters. Cluster I (strains 785T and 208) had the greatest 16S rRNA gene sequence similarity to Arthrobacter citreus (98.6 and 98.7 %, respectively), Arthrobacter luteolus (98.0 and 98.1%, respectively), Arthrobacter gandavensis (97.9 and 98.0 %, respectively) and Arthrobacter koreensis (97.6 and 97.7 %, respectively). Likewise, cluster II (strains J391T and J915) had the highest sequence similarity to Arthrobacter ruber (98.6 and 98.3 %, respectively) and Arthrobacter agilis (98.1 and 97.9 %, respectively). Average nucleotide identity and the digital DNA–DNA hybridization values illustrated that the two type strains, 785T and J391T, represented two separate novel species that are distinct from all currently recognized species in the genus Arthrobacter . These strains had DNA G+C contents of 66.0–66.1 mol% (cluster I) and 68.0 mol% (cluster II). The chemotaxonomic properties of strains 785T and J391T were in line with those of the genus Arthrobacter : anteiso-C15:0 (79.3 and 40.8 %, respectively) as the major cellular fatty acid, MK-8(H2) (65.8 %) or MK-9(H2) (75.6 %) as the predominant respiratory quinone, a polar lipid profile comprising diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipids and phospholipid, and A3α or A4α as the cell wall peptidoglycan type. On the basis of our results, two novel species in the genus Arthrobacter are proposed, namely Arthrobacter yangruifuii sp. nov. (type strain, 785T=CGMCC 1.16725T=GDMCC 1.1592T=JCM 33491T) and Arthrobacter zhaoguopingii sp. nov. (type strain, J391T=CGMCC 1.17382T=GDMCC 1.1667T=JCM 33841T).

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“…The International Code of Nomenclature of Prokaryotes (ICNP) subcommittee for the family Neissericeae has been inactive since 1982 and there are no minimal standards. Therefore, we performed the phenotypic characteristics on the basis of recent Neisseria species descriptions [3,4,8,32]. Strains were grown on different growth media, including TSA plates, Columbia agar with 10% horse blood and Brain Heart Infusion (BHI).…”

Section: Methodsmentioning

confidence: 99%

Neisseria leonissp. nov. isolated from rabbits, reclassification ofUruburuella suis, Uruburuella testudinis, Kingella potus, Bergeriella denitrificansandMorococcus cerebrosusintoNeisseriagenus and reclassification ofNeisseria shayeganiiintoEikenellagenus

Martin

Favre-Rochex

Gorgette

et al. 2023

Preprint

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Genome sequence-based identification of two strains (3986T and 51.81) isolated from rabbits in France in 1972 and 1981 and deposited in the Collection of Institut Pasteur (CIP) has led to the description of a novel species in the genus Neisseria. The cells of both strains were non-motile, Gram-stain-negative and diplococcobacilli. Optimal growth on trypticase soy agar was recorded at 37C and pH 8.5 in aerobic conditions. Phylogeny based on 16S rRNA gene placed the strains close to Neisseria bacilliformis ATCC BAA-1200T (96.38%) nesting with the members of Neisseriaceae family. Furthermore, the phylogenetic analysis based on bac120 gene set from the Genome Taxonomy Database (GTDB) placed both strains within the monophyletic Neisseria clade, which also included type strains of Morococcus cerebrosus, Bergeriella denitrificans, Kingella potus, Uruburuella suis and Uruburuella testudinis. However, Neisseria shayeganii strain 871T was placed outside Neisseria clade and close to the members of Eikenella genus. Strains 3986T and 51.81 were placed in a branch distinct from all species of the genus Neisseria and exhibited the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values below the species demarcation values. In contrast, ANI value within the two strains was 96.9% confirming that they represent same species. The genomic DNA G+C content of strain 3986T was 56.92%. Based on the phylogenetic and phenotypic data, the strains 3986T and 51.81 represent a novel species of the genus Neisseria, for which the name Neisseria leonis sp. nov. is proposed (type strain 3986T = CIP 109994T = LMG 32907T). Additionally, based on phylogenetic analysis, DUS dialect and average amino acid identity (AAI) values, we also proposed the reclassification of Morococcus cerebrosus, Bergeriella denitrificans, Kingella potus, Uruburuella suis and Uruburuella testudinis into Neisseria genus and Neisseria shayeganii into Eikenella genus.

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Neisseria weixii sp. nov., isolated from rectal contents of Tibetan Plateau pika (Ochotona curzoniae)

Cited by 15 publications

References 27 publications

Neisseria meningitidis has acquired sequences within the capsule locus by horizontal genetic transfer

Neisseria meningitidis has acquired sequences within the capsule locus by horizontal genetic transfer

Arthrobacter yangruifuii sp. nov. and Arthrobacter zhaoguopingii sp. nov., two new members of the genus Arthrobacter

Neisseria leonissp. nov. isolated from rabbits, reclassification ofUruburuella suis, Uruburuella testudinis, Kingella potus, Bergeriella denitrificansandMorococcus cerebrosusintoNeisseriagenus and reclassification ofNeisseria shayeganiiintoEikenellagenus

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