Nematicidal Activity of Mint Aqueous Extracts against the Root-Knot Nematode Meloidogyne incognita

Abstract

The nematicidal activity and chemical characterization of aqueous extracts and essential oils of three mint species, namely, Mentha × piperita , Mentha spicata , and Mentha pulegium , were investigated. The phytochemical analysis of the essential oils was performed by means of GC-MS, whereas the aqueous extracts were analyzed by LC-MS. The most abundant terpenes were isomenthone, menthone, menthol, pulegone, and carvone, and the water extracts yielded mainly chlorogenic acid, salvianolic acid B, luteolin-7-O-rutinoside, and rosmarinic acid. The water extracts exhibited significant nematicidal activity against Meloidogyne incognita , and the EC50/72h values were calculated at 1005, 745, and 300 mg/L for M. × piperita, M. pulegium, and M. spicata, respectively. Only the essential oil from M. spicata showed a nematicidal activity with an EC50/72h of 358 mg/L. Interestingly, menthofuran and carvone showed EC50/48h values of 127 and 730 mg/L, respectively. On the other hand, salicylic acid, isolated in the aqueous extracts, exhibited EC50 values at 24 and 48 h of 298 ± 92 and 288 ± 79 mg/L, respectively.

Larvicidal, molluscicidal and nematicidal activities of essential oils and compounds from Foeniculum vulgare

“…and Thymus sp. (Lamiaceae) have also proven their efficacy against M. javanica (LC 50 ranging from 300 to 204 mg l -1 ) (Andrés et al 2012) and M. incognita (LC 50 between 300 and 1,000 mg l -1 for EO or water extract from mints), another root-knot nematode of utmost importance (Caboni et al 2013a).…”

“…The purified compounds myristicin (Jaiswal and Singh 2009), limonene and eugenol (Kumar and Singh 2006) were identified as noteworthy molluscicides against L. acuminata (LC 50 = 1.5, 5.8 and 11 mg l -1 after 24 h, respectively). Generally, oxygenated compounds, namely ketones (L-carvone and pulegone), alcohols (geraniol and terpinen-4-ol) and phenols (eugenol, carvacrol and thymol), have revealed good nematotoxic properties (LC 50 from 115 to 754 mg l -1 after 24 h) (Ntalli et al 2010;Caboni et al 2013a). Volatile aromatic aldehydes, including benzaldehyde derivatives, have been recognized for their outstanding nematotoxicity against J 2 of M. javanica (50 % paralysis for concentrations between 13 and 33 ll l -1 after 48 h) (Oka 2001) and M. incognita (LC 50 between 11 and 167 mg l -1 after 24 h) (Caboni et al 2013b).…”

Management of foliar nematode Aphelenchoides ritzemabosi on Anemone hupehensis using plant extracts and pesticides

“…Among biological products, the nematicidal properties were revealed by an aqueous bulb extract of Allium sativum L. (Tibugari et al 2012;El-Nagdi and Youssef 2013), extracts of the soapbark tree-Quillaja saponaria Molina (Insunza et al 2001;San Martin and Magunacelaya 2005), extracts of Mentha sp. L. (Caboni et al 2013), oils of Syzygium aromaticum (L.) Merrill and Perry and Thymus vulgaris L. (Elbadri et al 2008), abamectin-produced by an actinomycete bacterium Streptomyces avermitilis (ex Burg et al 1979) Goodfellow 2002 (James et al 2006;Korayem et al 2008) and azadirachtin isolated from the neem tree Azadirachta indica A. Juss. (Lynn et al 2010;Khan et al 2012).…”

Evaluation of chemical alternatives to methyl bromide in tomato crops in China

“…Among substitutive chemicals, currently registered alternatives to MB are chloropicrin (CP); 1,3-dichloropropene (1,3-D); methyl isothiocyanate (MITC) generators such as Metam sodium (MNa) and Dazomet (DZ), abamectin (AB) and their combinations (Qiao et al, 2012;Mao et al, 2012;Yan et al, 2012). Other alternatives to MB fumigation may be the use of non-chemical methods, such as soil solarization, organic amendments and biocontrol agents (Klein et al, 2011;Caboni et al, 2013;Hu et al, 2013). However, nonchemical control methods alone are often unsuitable because they do not provide the broad-spectrum activity or the degree of consistency achieved with MB fumigation (Chellemi, 2002).…”