Nematicidal Effect of A Wild Type of Serratia Marcescens and Its Mutants Against Meloidogyne Incognita Juveniles

Kassab, Samir Oliveira; Eissa, M. F. M.; Badr, Usama M.; Ismail, A. E.; Razik, A. Abdel; Soliman, Gaziea

doi:10.21608/ejaj.2017.56204

Cited by 8 publications

(11 citation statements)

References 17 publications

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“…As in the present study, the species B. wiedmannii was one of the most effective species against M. incognita. In the case of Serratia, there is some studies about anti-nematode effects of S. marcescens against Meloidogyne (Mahfouz et al 2010;Kassab Hegazy et al 2019), and in the present study, the species S. liquefaciens as an endophytic bacteria was one of the effective species against M. incognita.…”

Section: Discussionsupporting

confidence: 62%

Potential of some endophytic bacteria in biological control of root-knot nematode Meloidogyne incognita

Moslehi

Pourmehr

Shirzad

et al. 2021

Egypt J Biol Pest Control

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Background Root-knot nematodes (Meloidogyne spp.) are among the most important plant pathogens. Biological control is one of the safety and effective methods for control of these nematodes. The aim of the present study was the isolation and identification of endophytic bacteria from tomato fields in some areas of Iran in order to evaluate their biocontrol potential against root-knot nematode. In the present study, the population of Meloidogyne was collected from infected cucumber roots of a greenhouse, and the bacteria were isolated from tomato samples collected from the fields in West-Azarbaijan province of Iran. The effects of the bacterial isolates on hatching and mortality of nematode second-stage juveniles were evaluated, and the effects of six selected isolates on infection of a susceptible cultivar of tomato with nematode were evaluated under greenhouse conditions. Results The root-knot nematode was identified as Meloidogyne incognita. Results showed that the all isolates exhibited considerable inhibitory effects on nematode hatching. The bacterial isolates also caused the mortality of juveniles. Six bacterial isolates with notable biocontrol potential were selected, and supplementary experiments and molecular identification of selected bacterial isolates were performed. Isolates 1, 2, 5, 7, 10, and 11 were identified as follows: Bacillus wiedmannii (MW405861), Pseudoxantomonas mexicana (MW405860), Pseudomonas thivervalensis (MW405862), Serratia liquefaciens (MW405864), Pseudomonas chlororaphis (MW405863), and P. fluorescens (MW405825), respectively. Based on the results of the greenhouse experiment, the selected isolates exhibited good results in terms of reduction of galls and egg masses of nematode. However, B. wiedmannii and S. liquefaciens had the best results in reduction of all investigated factors compared to other isolates. P. mexicana, P. chlororaphis, and P. fluorescens resulted moderate effects. P. thivervalensis was less effective than the others and in some cases had no effect on nematode reduction. Conclusions The results showed that endophytic bacteria are good candidates for management of root-knot nematodes. The use of such agents instead of chemicals will be very valuable to the control of nematodes.

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Section: Discussionsupporting

confidence: 62%

Potential of some endophytic bacteria in biological control of root-knot nematode Meloidogyne incognita

Moslehi

Pourmehr

Shirzad

et al. 2021

Egypt J Biol Pest Control

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“…30 and 59.43%, consecutively. The impacts of S. marcescens against the plant-parasitic nematodes may be attributed to the secretion of a variety of extracellular enzymes, including chitinase that degrade chitin (Hines et al, 1988;Kassab et al, 2017). Moreover, plants treated with S. marcescens induced protein content, chitinase, and peroxidase activities in treated plants as compared to untreated (Abd-Elgawad & Kabeil, 2010).…”

Section: Slope ± Variancementioning

confidence: 99%

Effects of vermicompost, vermicompost tea and a bacterial bioagent against Meloidogyne incognita on banana in Egypt

Awad-Allah¹,

Khalil²

2019

PJN

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The nematicidal potential of vermicompost (VC), vermicompost tea (VCT) and the bacterial bioagent Nemaless ® was evaluated against Meloidogyne incognita infecting banana (Musa acuminata) cv. "Grand Nain" as compared to the nematicide fenamiphos 40% EC under laboratory and field conditions. Experiments were carried-out during two successive seasons (2016 and 2017). Results of the laboratory tests showed that the toxicity of VCT and Nemaless ® to M. incognita second-stage juveniles (J 2 ) was increased as their concentrations and exposure times were increased. The LC 50 of VCT ranged from 30.86 × 10 3 to 83.77 × 10 5 mg/l, while the LC 50 of Nemaless ® ranged from 2.69 × 10 8 to 3.17 × 10 8 cell/ml. Results from the field experiments revealed that all of the tested treatments greatly suppressed (P ≤ 0.05) the numbers of galls, egg-masses and nematode final populations. The most potent materials in reducing the numbers of M. incognita in banana soil, in a descending order, were: fenamiphos, Nemaless ® , vermicompost and vermicompost tea (VCT).

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“…Chitinase is involved in inducing plant resistance against plant pathogen attack. The chitinase enzyme activity in suppressing the plant-parasitic nematodes has been reported by Gupta et al (2017) and Kassab et al (2017). Besides chitinase, another enzyme that contributes to suppress Meloidogyne spp.…”

Section: Discussionmentioning

confidence: 93%

Cost-effective bacteria-based bionematicide formula to control the root-knot nematode Meloidogyne spp. on tomato plants

et al. 2021

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Abstract. Asyiah IN, Prihatin J, Hastuti AD, Winarso S, Widjayanthi L, Nugroho D, Firmansyah K, Pradana AP. 2021. Cost-effective bacteria-based bionematicide formula to control root-knot nematode Meloidogyne spp. in tomato plants. Biodiversitas 22: 3256-3264. The root-knot nematode, Meloidogyne spp. can infect and cause loss production in various horticultural plants, including tomatoes. In the previous study, we found 3 endophytic bacteria isolates and 1 rhizobacterium isolate that could control several plant-parasitic nematodes. In this study, we formulated these bionematicide isolates with cheap and environmentally friendly organic materials. The formula was fortified using several organic matters, vitamin sources, protein sources, and sugar sources. The research was conducted in an experimental land with a history of severe root-knot nematode infection. The analysis showed that there were 63.7 J2 Meloidogyne spp. per 100 ml of soil on the experimental land. The application was given at a time interval of 2 weeks at the concentration of 0.5%, 1%, 1.5%, and 2%, with a dose of 100 ml per plant. As a negative control, the plant did not give any treatments, and as a positive control, the plant was given 5 g carbofuran per plant. The results revealed that treatment with 2% bionematicide formula concentration showed the best consistent result. This treatment increased canopy wet weight by 38.63% and root dry weight by 106.97% compared to negative control. The P4 treatment was also found effective to increase fruit weight by 33.61% and fruit diameter by 26.16% as compared to negative control. Increased plant growth in P4 treatment was closely related to the total of root-knot suppression and root damage intensity. In the P4 treatment, the total of root-knots and root damage intensities was 44.83% and 32.66%, respectively, compared to the negative control. This suppression also occurred in the nematode population and nematode eggs. In the P4 treatment, the total of Meloidogyne spp. J2 in soil and root was lower by 60.74% and 66.24%, respectively, compared to the negative control. A similar phenomenon also occurred in the total of eggs, which was 79.40% lower than the total of eggs in the negative control. This study provides the latest information about a cost-effective bacteria-based bionematicide formula, which is effective in suppressing Meloidogyne spp. infection in tomato, and promotes the growth and development tomato plant.

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Nematicidal Effect of A Wild Type of Serratia Marcescens and Its Mutants Against Meloidogyne Incognita Juveniles

Cited by 8 publications

References 17 publications

Potential of some endophytic bacteria in biological control of root-knot nematode Meloidogyne incognita

Potential of some endophytic bacteria in biological control of root-knot nematode Meloidogyne incognita

Effects of vermicompost, vermicompost tea and a bacterial bioagent against Meloidogyne incognita on banana in Egypt

Cost-effective bacteria-based bionematicide formula to control the root-knot nematode Meloidogyne spp. on tomato plants

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