Nematode resistance in bananas: screening results on some wild and cultivated accessions of Musa spp.

Abstract

Bananas cultivated for export all belong to Cavendish cultivars and are all recognized as very susceptible to nematodes, particularly to the burrowing nematode Radopholus similis and the lesion nematode Pratylenchus coffeae. Even if there have been many changes in the management of banana nematodes in large commercial banana plantations, chemical control still remains most often the last resort method to manage the nematodes, although the number of registered products is definitely declining. Therefore, nematode control though genetic improvement is gaining new interest worldwide. In this study, 55 banana accessions mostly diploids from the Musa acuminata genome group (AA) but including some triploid accessions (AAA), some diploids of the Musa balbisiana genome group (BB) and some interspecific hybrids (AAB, AB) were evaluated for resistance to four nematode species R. similis, P. coffeae, Meloidogyne incognita and M. arenaria. These experiments were conducted in a growth chamber under controlled conditions. All banana accessions were susceptible to nematode species, although many different levels of susceptibility were detected. This study confirmed the good resistance status to R. similis of some cultivars from the Pisang jari buaya and Pisang batuau subgroups and the partial resistance of 17 diploid accessions significantly different from the susceptible reference cv. Grande Naine. This study also showed that 12 diploid accessions exhibited a partial resistance to P. coffeae, including some usual or potential genitors belonging to the wild diploids subspecies burmannica (cvs. Long Tavoy 1 and 2) and burmannicoides (cv. Calcutta 4). No source of resistance to Meloidogyne spp. was found. These screening results, combining for the first time four nematode species, are discussed within the scope of banana breeding in order to produce parental diploid lines with single or combined nematode resistances and further develop triploids that can substitute existing susceptible commercial cultivars.

Gene expression analysis inMusa acuminataduring compatible interactions withMeloidogyne incognita

“…(e.g. Stoffelen et al, 2000;Van den Bergh et al, 2002a, b;Quénéhervé et al, 2009), effective sources of resistance remain lacking in breeding programmes. Complementing recent characterization of gene expression during a Musa-nematode incompatible interaction, namely with the migratory endoparasitic nematode, Pratylenchus coffeae (Backiyarani et al, 2014), this data set for the M. acuminata-M. incognita compatible interaction also represents an important addition to existing transcriptome resources for M. acuminata-pathogen interactions (Li et al, 2012;Bai et al, 2013;Passos et al, 2013).…”

“…In banana, however, sources of genetic resistance to RKNs are limited, with very few reports of inclusion of promising genotypes with effective resistance or tolerance in Musa breeding programmes (Davide and Marasigan, 1985;Vilas Boas et al, 2002;Quénéhervé et al, 2009).…”

Nematodos fitoparásitos asociados a raíces de plátano (Musa acuminata AA) en el centro de Veracruz, México

“…This may be due to the origin and variety of banana plants in this area (Musa acuminata AA subgroup Pisang Mas). This statement is based on the findings that show that many diploid accessions, such as the studied plants, are less susceptible to R. similis (Quénéhervé et al, 2009). Also, the temperature rank in the study area (14-26 °C) is low for the optimal development of R. similis (24-32 °C) (Pinochet et al, 1995;Gowen et al, 2005) and can contribute to a higher presence of Helicotylenchus and Meloidogyne.…”

“…El nematodo parásito más perjudicial y distribuido en el mundo en raíces de plátano es Radopholus similis (Chabrier y Queneherve, 2003;Quénéhervé et al, 2011), este se encontró en cuatro de los seis sitios examinados, aunque en bajas poblaciones, esto puede deberse al origen y la variedad de las plantas cultivadas en esta zona (Musa acuminata AA subgrupo Pisang Mas) ya que se ha demostrado que muchas accesiones diploides como las plantas estudiadas en este trabajo, son menos susceptibles a R. similis (Quénéhervé et al, 2009). Asimismo el rango de temperaturas de la zona de estudio (14-26 °C) es bajo para el óptimo desarrollo de R. similis (24-32 °C) (Pinochet et al, 1995;Gowen et al, 2005) lo que puede influir en la mayor presencia de Helicotylenchus y Meloidogyne.…”

Effect of preharvest and postharvest application of calcium on banana green-life

“…Cavendish Grande Naine) and on a new triploid hybrid (Flhorban920 cultivar, AAA group). This hybrid is partially resistant to Yellow Sigatoka and Black Leaf Streak Disease [11]; it presents good tolerance to plant parasitic nematodes [12,13], and has sensory and nutritional characteristics that are different from those of Cavendish banana [14]. Such triploids are issued from conventional breeding techniques [15], including diploid resistant genitors previously doubled with colchicine [16].…”