Neochamaejasmin A extracted from Stellera chamaejasme L. induces apoptosis involving mitochondrial dysfunction and oxidative stress in Sf9 cells

Ren, Yuanhang; Mu, Yangping; Jin, Hong; Tao, Ke; Hou, Taiping

doi:10.1016/j.pestbp.2019.03.025

Cited by 13 publications

(20 citation statements)

References 33 publications

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“…Apoptosis-associated changes were examined by using an annexin V-EGFP/PI apoptosis detection kit (Keygen Biotech Co., Ltd) according to previously reported methods. 12 The incubation cells were exposed to ICM at various concentrations (20,40, and 80 μmol L -1 ) for 24 h (0.1% DMSO for controls).…”

Section: Apoptosis Assaymentioning

confidence: 99%

“…The Rh123 and JC-1 assay kits (Keygen Biotech Co., Ltd) were used to quantify the mitochondrial membrane potential (MMP), following a previously reported method. 12 The incubation cells were exposed to ICM with various concentrations (20,40, and 80 μmol L -1 ) for 24 h (0.1% DMSO for controls).…”

Section: Measurement Of Mitochondrial Membrane Potential (Mmp)mentioning

confidence: 99%

“…The antibodies against Bax, Bcl-2, PARP, cyt-c, OGG1, γH2AX, and GAPDH (Abgent, China) were used in western blotting assay of ICM on AW1 cells; evaluations were carried out according to previously reported methods. 12,13 The incubation cells were exposed to ICM at various concentrations (20, 40 and 80 μmol L -1 ) for 24 h (0.1% DMSO for controls). wileyonlinelibrary.com/journal/ps 2.11 Measurement of caspase-3 and caspase-9 activities The activity of caspase-3 and caspase-9 was evaluated by using the Caspase-3/9 assay kit (Keygen Biotech Co., Ltd), according to previously reported methodology.…”

Section: Western Blottingmentioning

confidence: 99%

“…wileyonlinelibrary.com/journal/ps 2.11 Measurement of caspase-3 and caspase-9 activities The activity of caspase-3 and caspase-9 was evaluated by using the Caspase-3/9 assay kit (Keygen Biotech Co., Ltd), according to previously reported methodology. 12 The incubation cells were exposed to ICM at 20 μmol L -1 for various amounts of time (6, 12, and 24 h).…”

Section: Western Blottingmentioning

confidence: 99%

“…The activity of superoxide dismutase (SOD), glutathione Stransferase (GST), catalase (CAT), and the content of malondialdehyde (MDA) was carried out using corresponding detection kits (Beyotime Biotechnology) according to previously reported operation methods. 12,[14][15][16][17] The incubation cells were exposed to ICM at various concentrations (20,40, and 80 μmol L -1 ) for 24 h (0.1% DMSO for controls).…”

Section: Measurement Of Sod Gst Cat and Mdamentioning

confidence: 99%

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Isochamaejasmin induces toxic effects onHelicoverpa zeaviaDNAdamage and mitochondria‐associated apoptosis

Ren

et al. 2020

Pest Management Science

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BACKGROUND: Stellera chamaejasme L. is a poisonous plant with rich resources and is thus highly valuable in terms of new pesticide development. Isochamaejasmin (ICM), one of the main ingredients in S. chamaejasme has drawn much attention owing to its antitumour properties. However, the toxicity and mode of action of ICM on insects are still not clear. In this article, the larva and neuronal cell (AW1) of Helicoverpa zea were used to clarify the insecticidal activity of ICM as well as its toxic mechanism at the cellular level. RESULTS: The results confirmed that ICM has potential toxicity against H. zea both in vivo and in vitro via time-and dosedependent manners. Moreover, we found that ICM caused DNA damage and increased the levels of γH2AX and OGG1 in AW1 cells. Results also showed decline in the mitochondrial membrane potential (MMP), upregulation of Bax/Bcl-2 expression resulting in the release of cytochrome c into the cytosol, activation of caspase-3/9, and cleavage of poly ADP-ribose polymerase (PARP) as a result of exposure to ICM. Additionally, a dose-dependent rise in the reactive oxygen species (ROS) levels, accumulation of a lipid peroxidation product, and inactivation of antioxidant enzymes were found in ICM-treated cells. CONCLUSION: These findings confirmed the insecticidal activity of ICM. Furthermore, the results revealed that ICM could cause DNA damage and induce apoptosis via the mitochondrial pathway in AW1 cells. This study provides the basic information needed to understand the toxicity and mechanisms of action of ICM, which could potentially be used to develop it as a new insecticide.

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Section: Apoptosis Assaymentioning

confidence: 99%

Section: Measurement Of Mitochondrial Membrane Potential (Mmp)mentioning

confidence: 99%

Section: Western Blottingmentioning

confidence: 99%

Section: Western Blottingmentioning

confidence: 99%

Section: Measurement Of Sod Gst Cat and Mdamentioning

confidence: 99%

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Isochamaejasmin induces toxic effects onHelicoverpa zeaviaDNAdamage and mitochondria‐associated apoptosis

Ren

et al. 2020

Pest Management Science

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Neochamaejasmin B extracted from Stellera chamaejasme L. induces apoptosis through caspase‐10‐dependent way in insect neuronal cells

Jiang

et al. 2022

Arch Insect Biochem Physiol

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To explore the toxicity mechanisms of neochamaejasmin B (NCB) extracted from Stellera chamaejasme L., we first evaluated its cytotoxicity in neuronal cells of Helicoverpa zea (AW1 cells). NCB inhibited cell growth and was cytotoxic to AW1 cells in a dose-dependent manner.Further, transmission electron microscopy (TEM) was used to analyze the microstructure, and typical apoptotic characteristics were observed in AW1 cells treated with NCB. Moreover, the NCB-induced apoptosis was dose dependent. Subsequently, we explored the mechanism of apoptosis. A decline in the mitochondrial membrane potential (MMP) was found. Also, the levels of Bax were increased with increases in drug concentration, but there was no statistical difference in Bcl-2 levels at different NCB doses. Caspase-3 and caspase-10 activity was increased.These findings confirmed that NCB induced apoptosis in AW1 cells through a caspase-10-dependent mechanism.The results provide the basic information needed for understanding the toxicity and mechanisms of action of NCB, which could potentially be used to develop NCB as a new insecticide.

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Avermectin induces toxic effects in insect nontarget cells involves DNA damage and its associated programmed cell death

Zhang

et al. 2021

Comparative Biochemistry and Physiology Part C: Toxicology &

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Neochamaejasmin A extracted from Stellera chamaejasme L. induces apoptosis involving mitochondrial dysfunction and oxidative stress in Sf9 cells

Cited by 13 publications

References 33 publications

Isochamaejasmin induces toxic effects onHelicoverpa zeaviaDNAdamage and mitochondria‐associated apoptosis

Isochamaejasmin induces toxic effects onHelicoverpa zeaviaDNAdamage and mitochondria‐associated apoptosis

Neochamaejasmin B extracted from Stellera chamaejasme L. induces apoptosis through caspase‐10‐dependent way in insect neuronal cells

Avermectin induces toxic effects in insect nontarget cells involves DNA damage and its associated programmed cell death

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