Neonatal Detection of Hypothyroidism by Radioimmunoassay of the Thyroxin in the Eluate of Dried Blood

Abstract

e c t . P r e l h i m r y data o f a s y s t e n n t i c s c r e e n i n g of neonates a r e presented. The v a l u e s o f senun thyroxine (T4) and TSH were determined in the c o r d b l c c d o f 271 i n f a n t s . I n 152 o f these c h i l d r e n serumTSH was a g a i n measured o n a f i n g e r puncture sample a t 5 days o f age. A t b i r t h the v a r i a b i l i t y is irqx~rtant f o r T4 and TSH. On th6 5th day the s c a t t e r i n g o f TSH v a l u e s is much srraller and t h e rrrean is lower than a t b i r t h (< 0.031). In agreement w i t h previo u s l y r e p r t e d d a t a we found serum TSH l e v e l s o f 160 and 5CO U/d i n 2 hypothyroid i n f a n t s younger t h a n 1 m n t h . The strikin: d i f f e r e n c e beedeen t h e s e high TSH v a l u e s and the c o n t r o l v a l u e s observed o n the 5th day a l l m to s u p p r t the v i e d that this s c r e e n i n g procedure is r e l i a b l e f o r d e t e c t i n g c o n g e n i t a l kypthiTr. s a y a r i and D.Augier. S e r v i c e de MGdecine I n f a n t i l e C , U.E.R.Toulouse-R a n g u e i l . F r a n c e . I n 5 c o n f i r m e d h y p o t h y r o i d i a n s i n f a n t s t h e a v e r a g e is 160 ' 6 0 pg. I n 2 c a s e s o f a t h y r e o s i s t h e a v e r a g e i s 8 0 pg. T h i s method was u s e d f o r d e t e c t i o n o f h y p o t h y r o i d i s m i n one thousand o f newbornsby u s i n g s a m p l i n g s o b t a i n e d from t h e p h e n y l c e t o n u r i c d e t e c t i o n c e n t e r . We d e t e c t e d by t h i s way a c a s e of h y p o t h y r o i d i s m which w a s confirmed by t r a d i t i o n a l t e s t s . T h i s method seems t o t o t a l l y j u s t i f y i n g a n e o n a t a l d e t e c t i o n of h y p o t h y r o i d i s m on a b i g s c a l e . NEONATAL DETECTION OF HYPOTHYROIDISM BY R The a s s a y of T h y r o x i n (T4) h a s been made by radio-immunoassly i n t h e e l u a t e o f a few d r o p s o f b l o o d d e p o s i t e d on a p i e c e o f b l o t t i n GOITER DUE TO CONGENITAL DYSHORMOGENESIS: 32 FOLLOW UP STUDY IN 28 CHILDREN. Sonia lorcansky, Noe Altschuler, Cesar Bergad; and Martin Cullen. Serv. Endocr., Hosp. de Niiios, Buenos Aires, ArgentinoWe have studied 17 boys and 11 girls ( 7 pairs of siblings) with goiter over a period of 2-15 yrs. Methods : In all patients, 13lI-uptoke, PB1I3l, CR, SCNKdischarge, PBI, BE1 or Tq, column chromatograms of radioiodinated aminoacids in serum have been performed ; in some, chromatograms have been done olso in thyroid tissue and urine.Based on laboratory data, 5 groups have been formed. Althoughsynthesis of thyroid hormones is blocked in all enzyme defects, the course of the disease is varioble but charocteristic for each group : 1) in 7 of 8 children with complete peroxydase defect, brain damage and idiocy developed despite thyroid theropy beginning shortly after birth. 2)4 patients with Pendred s ndrome were euthyroid and had smoll goiters ; of 2 sisters with the some J i n i c a l picture,one showed the typical 1'31 discharge wherea...

Serum thyrotrophin determination on day 5 of life as screening procedure for congenital hypothyroidism.

“…Dussault and Laberge (1973) developed a screening technique based on radioimmunoassay of thyroxine (T4) on the 5th day of life from an eluate of dried blood. This technique was later adapted and modified by several teams (Walfish et al, 1975;Rochiccioli et al, 1975;Larsen and Broskin, 1975). Others use measurements of thyrotrophin (TSH) in cord blood (Klein et al, 1974;Walfish et al, 1975).…”