Neonatal Development of the Stratum Corneum pH Gradient: Localization and Mechanisms Leading to Emergence of Optimal Barrier Function

Behne, Martin J.; Barry, Nicolas P. E.; Hanson, Kyle C.; Aronchik, Ida; Clegg, Robert W.; Gratton, Enrico; Kr, Feingold; Holleran, Walter M.; Elias, Peter M.; Mauro, Theodora M.

doi:10.1046/j.1523-1747.2003.12262.x

Cited by 46 publications

(54 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is likely that reduced ␤-glucocerebrosidase activity is causative for the ceramides modifications in the ␣ENaC knockout group. Furthermore, a neutral skin surface pH has been proposed to cause incomplete lipid processing within the early postnatal period (47). Our data indicate, rather, that the lipid abnormalities are the primary defect (Fig.…”

Section: Enac Is Important In the Maintenance Of The Epidermal Permeamentioning

confidence: 57%

Postnatal Requirement of the Epithelial Sodium Channel for Maintenance of Epidermal Barrier Function

Charles

Guitard

Leyvraz

et al. 2008

Journal of Biological Chemistry

View full text Add to dashboard Cite

In skin, the physiological consequence of an epithelial sodium channel (ENaC) deficiency is not obvious directly at birth. Nevertheless, within hours after birth, mice deficient for the ␣-subunit of the highly amiloride-sensitive epithelial sodium channel (␣ENaC/Scnn1a) suffer from a significant increased dehydration. This is characterized by a loss of body weight (by 6% in 6 h) and an increased transepidermal water loss, which is accompanied by a higher skin surface pH in 1-day-old pups. Although early and late differentiation markers, as well as tight junction protein distribution and function, seem unaffected, deficiency of ␣ENaC severely disturbs the stratum corneum lipid composition with decreased ceramide and cholesterol levels, and increased pro-barrier lipids, whereas covalently bound lipids are drastically reduced. Ultrastructural analysis revealed morphological changes in the formation of intercellular lamellar lipids and the lamellar body secretion. Extracellular formation of the lamellar lipids proved to be abnormal in the knockouts. In conclusion, ENaC deficiency results in progressive dehydration and, consequently, weight loss due to severe impairment of lipid formation and secretion. Our data demonstrate that ENaC expression is required for the postnatal maintenance of the epidermal barrier function but not for its generation.

show abstract

Section: Enac Is Important In the Maintenance Of The Epidermal Permeamentioning

confidence: 57%

Postnatal Requirement of the Epithelial Sodium Channel for Maintenance of Epidermal Barrier Function

Charles

Guitard

Leyvraz

et al. 2008

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…148 In human subjects the skin surface pH at birth is near neutral (pH 6.5) compared with that in children and adults. [149][150][151] In newborn rats the stratum corneum reaches adult pH levels during the first few days after birth, 152,153 whereas similar changes take a few weeks to occur in human newborns. 151,154 Although the acid mantle of the stratum corneum was initially thought to originate from exogenous sources (microbial metabolites, free fatty acids of pilosebaceous origin, and eccrine gland-derived products, such as amino and lactic acids), [155][156][157] recent studies have demonstrated that endogenous pathways (generation of byproducts of keratinization, synthesis of free fatty acids from phospholipid hydrolysis by the secretory phospholipase A 2 and the non-energy-dependent sodium-proton exchanger) are additional sources.…”

Section: Gene-environment Interactions: Ph and Detergentsmentioning

confidence: 99%

New perspectives on epidermal barrier dysfunction in atopic dermatitis: Gene–environment interactions

Cork

Robinson

Vasilopoulos

et al. 2006

Journal of Allergy and Clinical Immunology

465

335

View full text Add to dashboard Cite

“…Choi et al showed that the increased vulnerability of aged skin is due to abnormal SC acidity, resulting in defective lipid processing and loss of SC integrity [16]. Table 1 summarizes known changes in epidermal barrier function during aging [9,16,17,18,19,20,21,22,23,24], which may affect pH SC and pH SS .…”

Section: Introductionmentioning

confidence: 99%

Impact of Age and Body Site on Adult Female Skin Surface pH

et al. 2012

View full text Add to dashboard Cite

Background: pH is known as an important parameter in epidermal barrier function and homeostasis. Aim: The impact of age and body site on skin surface pH (pH_SS) of women was evaluated in vivo. Methods: Time domain dual lifetime referencing with luminescent sensor foils was used for pH_SS measurements. pH_SS was measured on the forehead, the temple, and the volar forearm of adult females (n = 97, 52.87 ± 18.58 years, 20–97 years). Every single measurement contained 2,500 pH values due to the luminescence imaging technique used. Results: pH_SS slightly increases with age on all three investigated body sites. There are no significant differences in pH_SS between the three investigated body sites. Conclusion: Adult pH_SS on the forehead, the temple and the volar forearm increases slightly with age. This knowledge is crucial for adapting medical skin care products.

show abstract

Neonatal Development of the Stratum Corneum pH Gradient: Localization and Mechanisms Leading to Emergence of Optimal Barrier Function

Cited by 46 publications

References 50 publications

Postnatal Requirement of the Epithelial Sodium Channel for Maintenance of Epidermal Barrier Function

Postnatal Requirement of the Epithelial Sodium Channel for Maintenance of Epidermal Barrier Function

New perspectives on epidermal barrier dysfunction in atopic dermatitis: Gene–environment interactions

Impact of Age and Body Site on Adult Female Skin Surface pH

Contact Info

Product

Resources

About